FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387394224> ?p ?o ?g. }

• W4387394224 abstract "The market demand for β-nicotinamide mononucleotide (NMN), an antiaging health product, is rapidly expanding. Consequently, there is an urgent requirement for an environment-friendly and economical approach to synthesize NMN. In this study, we develop a biocatalytic synthesis method for the production of valuable NMN from chemically synthesized nicotinamide riboside (NR). To enhance the efficiency of the process, the biocatalyst human nicotinamide riboside kinase (HS-NRK) was engineered by introducing a beneficial substitution (G13S) near the ATP-binding site. As a result, a 100% conversion of 50 g L–1 NR was achieved by using the engineered variant NRKG13S. However, the NMN yield was limited to 78% (39 g L–1) due to biodegradation by endogenous enzymes present in Escherichia coli. To overcome this challenge, we identified and knocked out the deoD gene for NMN biodegradation. Through protein engineering and metabolic engineering, we achieved a high NMN yield of 93% within 2 h using an effective whole-cell biocatalysis approach. Finally, an immobilized biocatalyst in continuous operation was developed, which facilitated the enzymatic process and enabled the cost-effective and environmentally attractive production of NMN." @default.
• W4387394224 created "2023-10-07" @default.
• W4387394224 creator A5000622559 @default.
• W4387394224 creator A5008017308 @default.
• W4387394224 creator A5017427697 @default.
• W4387394224 creator A5024847477 @default.
• W4387394224 creator A5049584538 @default.
• W4387394224 creator A5050323272 @default.
• W4387394224 creator A5058627154 @default.
• W4387394224 creator A5089034091 @default.
• W4387394224 date "2023-10-06" @default.
• W4387394224 modified "2023-10-18" @default.
• W4387394224 title "Streamlining Design, Engineering, and Applications of Nicotinamide Riboside Kinase for Sustainable Biosynthesis of Nicotinamide Mononucleotide in Flow" @default.
• W4387394224 cites W2015799885 @default.
• W4387394224 cites W2050588192 @default.
• W4387394224 cites W2095563155 @default.
• W4387394224 cites W2098949725 @default.
• W4387394224 cites W2099099776 @default.
• W4387394224 cites W2114520383 @default.
• W4387394224 cites W2155518645 @default.
• W4387394224 cites W2168974130 @default.
• W4387394224 cites W2531686927 @default.
• W4387394224 cites W2558718647 @default.
• W4387394224 cites W2565616863 @default.
• W4387394224 cites W2618669287 @default.
• W4387394224 cites W2775008495 @default.
• W4387394224 cites W2801249406 @default.
• W4387394224 cites W2810605473 @default.
• W4387394224 cites W2885979447 @default.
• W4387394224 cites W2887854403 @default.
• W4387394224 cites W2897600200 @default.
• W4387394224 cites W2902471330 @default.
• W4387394224 cites W2902905911 @default.
• W4387394224 cites W2946683133 @default.
• W4387394224 cites W2955882179 @default.
• W4387394224 cites W2963580194 @default.
• W4387394224 cites W2964120378 @default.
• W4387394224 cites W2970027515 @default.
• W4387394224 cites W2989621922 @default.
• W4387394224 cites W3023781577 @default.
• W4387394224 cites W3044425885 @default.
• W4387394224 cites W3081144730 @default.
• W4387394224 cites W3101695833 @default.
• W4387394224 cites W3136988020 @default.
• W4387394224 cites W3152001923 @default.
• W4387394224 cites W3166615178 @default.
• W4387394224 cites W3171261595 @default.
• W4387394224 cites W3183344282 @default.
• W4387394224 cites W3184513615 @default.
• W4387394224 cites W3188277748 @default.
• W4387394224 cites W4207077259 @default.
• W4387394224 cites W4210718771 @default.
• W4387394224 cites W4220763461 @default.
• W4387394224 cites W4221000052 @default.
• W4387394224 cites W4225117517 @default.
• W4387394224 cites W4292148561 @default.
• W4387394224 cites W4293102775 @default.
• W4387394224 doi "https://doi.org/10.1021/acssuschemeng.3c02833" @default.
• W4387394224 hasPublicationYear "2023" @default.
• W4387394224 type Work @default.
• W4387394224 citedByCount "0" @default.
• W4387394224 crossrefType "journal-article" @default.
• W4387394224 hasAuthorship W4387394224A5000622559 @default.
• W4387394224 hasAuthorship W4387394224A5008017308 @default.
• W4387394224 hasAuthorship W4387394224A5017427697 @default.
• W4387394224 hasAuthorship W4387394224A5024847477 @default.
• W4387394224 hasAuthorship W4387394224A5049584538 @default.
• W4387394224 hasAuthorship W4387394224A5050323272 @default.
• W4387394224 hasAuthorship W4387394224A5058627154 @default.
• W4387394224 hasAuthorship W4387394224A5089034091 @default.
• W4387394224 hasConcept C161790260 @default.
• W4387394224 hasConcept C179720865 @default.
• W4387394224 hasConcept C181199279 @default.
• W4387394224 hasConcept C185592680 @default.
• W4387394224 hasConcept C191503008 @default.
• W4387394224 hasConcept C21951064 @default.
• W4387394224 hasConcept C2776328115 @default.
• W4387394224 hasConcept C2776901170 @default.
• W4387394224 hasConcept C2777088662 @default.
• W4387394224 hasConcept C2779728014 @default.
• W4387394224 hasConcept C2909408332 @default.
• W4387394224 hasConcept C55493867 @default.
• W4387394224 hasConcept C6350086 @default.
• W4387394224 hasConcept C65024703 @default.
• W4387394224 hasConcept C75520062 @default.
• W4387394224 hasConceptScore W4387394224C161790260 @default.
• W4387394224 hasConceptScore W4387394224C179720865 @default.
• W4387394224 hasConceptScore W4387394224C181199279 @default.
• W4387394224 hasConceptScore W4387394224C185592680 @default.
• W4387394224 hasConceptScore W4387394224C191503008 @default.
• W4387394224 hasConceptScore W4387394224C21951064 @default.
• W4387394224 hasConceptScore W4387394224C2776328115 @default.
• W4387394224 hasConceptScore W4387394224C2776901170 @default.
• W4387394224 hasConceptScore W4387394224C2777088662 @default.
• W4387394224 hasConceptScore W4387394224C2779728014 @default.
• W4387394224 hasConceptScore W4387394224C2909408332 @default.
• W4387394224 hasConceptScore W4387394224C55493867 @default.
• W4387394224 hasConceptScore W4387394224C6350086 @default.
• W4387394224 hasConceptScore W4387394224C65024703 @default.
• W4387394224 hasConceptScore W4387394224C75520062 @default.

• next