FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387396492> ?p ?o ?g. }

• W4387396492 endingPage "e132" @default.
• W4387396492 startingPage "e132" @default.
• W4387396492 abstract "Though more than 20% of reproductive aged women are affected by obesity, our understanding of its impact on the physiology of the human endometrium is limited. We sought to determine if obesity results in novel proteomic, transcriptional, and epigenetic alterations to the luteal phase endometrium in a well phenotyped cohort without other comorbidities. Normally cycling women between 18-40 years old classified as normal weight (NW), overweight (OW) or having obesity (OB) based on body mass index (BMI) were recruited, and samples from the University of Michigan Reproductive Subject Registry and Sample Repository (RSRSR) were used. Individuals on recent hormone treatment or with a history of PCOS, STDs, autoimmune diseases, endometriosis, intrauterine pathology, or infertility were excluded. Participants completed a baseline screening visit consisting of blood tests, saline infusion sonogram, anthropometric measurements, and bioelectrical impedance analysis (BIA). Mid-luteal phase (7 days post +OPK) endometrial biopsies were obtained. Protein, RNA and DNA were isolated for proteomics, bulk RNA sequencing and DNA methylation (methylation, or mc, and hydroxymethylation, or hMC), respectively. Western blot (WB) and real time-PCR validation of the differentially expressed proteins/genes were performed. Differences between groups were analyzed using Student’s t-test, ANOVA or Chi-square tests. A total of 256 individuals were screened and 11 participants met the inclusion/exclusion criteria and completed a luteal biopsy (4 NW, 3 OW and 4 OB). Samples from individuals enrolled in RSRSR were used for validation. There were no significant differences in baseline demographics (age, gravidity, and parity), or endocrine/ovarian reserve profiles. Average BMI and % body fat were higher in OB (OB vs OW vs NW 37.1 ± 5.6 vs. 27.6 ± 2.1 vs 23.2 ± 1.0 kg/m2 and 43.6% vs. 30.5% vs 24.3%, respectively; p<0.05). A total of 360 differentially expressed proteins were identified (11 upregulated and 349 downregulated) in OB+OW vs NW (p<0.05). Two differentially expressed genes were detected in each cohort (FDR < 0.05, linear fold-change > 1.5). Compared to NW, OB+OW had 94 regions impacted by MC and 574 by hMC while OB alone had 125 regions affected by MC and 1,266 by hMC (p<0.005). One progesterone-related receptor was underexpressed in OB vs NW. Two sperm-related proteins were differentially expressed (over and underexpressed) in OB+OW vs NW. OB is associated with significant proteomic and epigenetic changes in the luteal endometrium of this extensively phenotyped cohort of women. Despite equivalent serum progesterone levels, the membrane-associated progesterone receptor (PGRMC1) was decreased in OB. We also found differential expression of two sperm-related proteins important for fertilization and early development in the female reproductive tract. Additional studies are needed to validate these findings, assess their clinical impact, and determine their reversibility with weight loss." @default.
• W4387396492 created "2023-10-07" @default.
• W4387396492 creator A5010312288 @default.
• W4387396492 creator A5023340631 @default.
• W4387396492 creator A5056548210 @default.
• W4387396492 creator A5057876588 @default.
• W4387396492 creator A5070644154 @default.
• W4387396492 creator A5091177547 @default.
• W4387396492 date "2023-10-01" @default.
• W4387396492 modified "2023-10-07" @default.
• W4387396492 title "OBESITY INDUCED LUTEAL PHASE ENDOMETRIAL PROFILE CHANGES IN A WELL-PHENOTYPED POPULATION" @default.
• W4387396492 doi "https://doi.org/10.1016/j.fertnstert.2023.08.411" @default.
• W4387396492 hasPublicationYear "2023" @default.
• W4387396492 type Work @default.
• W4387396492 citedByCount "0" @default.
• W4387396492 crossrefType "journal-article" @default.
• W4387396492 hasAuthorship W4387396492A5010312288 @default.
• W4387396492 hasAuthorship W4387396492A5023340631 @default.
• W4387396492 hasAuthorship W4387396492A5056548210 @default.
• W4387396492 hasAuthorship W4387396492A5057876588 @default.
• W4387396492 hasAuthorship W4387396492A5070644154 @default.
• W4387396492 hasAuthorship W4387396492A5091177547 @default.
• W4387396492 hasBestOaLocation W43873964921 @default.
• W4387396492 hasConcept C126322002 @default.
• W4387396492 hasConcept C131872663 @default.
• W4387396492 hasConcept C134018914 @default.
• W4387396492 hasConcept C143228043 @default.
• W4387396492 hasConcept C16685009 @default.
• W4387396492 hasConcept C187785154 @default.
• W4387396492 hasConcept C2776606343 @default.
• W4387396492 hasConcept C2777688143 @default.
• W4387396492 hasConcept C2779234561 @default.
• W4387396492 hasConcept C2779742232 @default.
• W4387396492 hasConcept C2780221984 @default.
• W4387396492 hasConcept C2780586474 @default.
• W4387396492 hasConcept C2908647359 @default.
• W4387396492 hasConcept C29456083 @default.
• W4387396492 hasConcept C42407357 @default.
• W4387396492 hasConcept C511355011 @default.
• W4387396492 hasConcept C54355233 @default.
• W4387396492 hasConcept C71924100 @default.
• W4387396492 hasConcept C86803240 @default.
• W4387396492 hasConcept C99454951 @default.
• W4387396492 hasConceptScore W4387396492C126322002 @default.
• W4387396492 hasConceptScore W4387396492C131872663 @default.
• W4387396492 hasConceptScore W4387396492C134018914 @default.
• W4387396492 hasConceptScore W4387396492C143228043 @default.
• W4387396492 hasConceptScore W4387396492C16685009 @default.
• W4387396492 hasConceptScore W4387396492C187785154 @default.
• W4387396492 hasConceptScore W4387396492C2776606343 @default.
• W4387396492 hasConceptScore W4387396492C2777688143 @default.
• W4387396492 hasConceptScore W4387396492C2779234561 @default.
• W4387396492 hasConceptScore W4387396492C2779742232 @default.
• W4387396492 hasConceptScore W4387396492C2780221984 @default.
• W4387396492 hasConceptScore W4387396492C2780586474 @default.
• W4387396492 hasConceptScore W4387396492C2908647359 @default.
• W4387396492 hasConceptScore W4387396492C29456083 @default.
• W4387396492 hasConceptScore W4387396492C42407357 @default.
• W4387396492 hasConceptScore W4387396492C511355011 @default.
• W4387396492 hasConceptScore W4387396492C54355233 @default.
• W4387396492 hasConceptScore W4387396492C71924100 @default.
• W4387396492 hasConceptScore W4387396492C86803240 @default.
• W4387396492 hasConceptScore W4387396492C99454951 @default.
• W4387396492 hasIssue "4" @default.
• W4387396492 hasLocation W43873964921 @default.
• W4387396492 hasOpenAccess W4387396492 @default.
• W4387396492 hasPrimaryLocation W43873964921 @default.
• W4387396492 hasRelatedWork W1675881499 @default.
• W4387396492 hasRelatedWork W1969938964 @default.
• W4387396492 hasRelatedWork W2004931656 @default.
• W4387396492 hasRelatedWork W2351089095 @default.
• W4387396492 hasRelatedWork W2360114324 @default.
• W4387396492 hasRelatedWork W2398412987 @default.
• W4387396492 hasRelatedWork W2400161738 @default.
• W4387396492 hasRelatedWork W2410429650 @default.
• W4387396492 hasRelatedWork W2975002074 @default.
• W4387396492 hasRelatedWork W82523391 @default.
• W4387396492 hasVolume "120" @default.
• W4387396492 isParatext "false" @default.
• W4387396492 isRetracted "false" @default.
• W4387396492 workType "article" @default.