FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387396502> ?p ?o ?g. }

• W4387396502 endingPage "e134" @default.
• W4387396502 startingPage "e134" @default.
• W4387396502 abstract "The ankyrin repeat and EF-hand domain containing protein 1 (Ankef1) has been found to be highly enriched in the testes, yet its function remains uncharacterized in mammals. An Ankef1 knockout (KO) mouse model was created by the CRISPR-cas9 system to help elucidate its function. The male mice homozygous for the Ankef1 knock out mutation were found to be sterile, however the sperm count and morphology was similar to wild type (WT) mice. The objective of this study is to characterize the function of Ankef1 and its role in male fertility. The sperm from Ankef1 deficient mice was examined by performing in vitro fertilization (IVF) studies using both heterozygous (Ankef1+/-) and homozygous knockout (Ankef1-/-) mouse sperm. IVF was performed using sperm that were collected from caudae epididymides into FHM medium and capacitated during incubation. Ovulation was induced by sequential intraperitoneal injection of Pregnant Mare Serum Gonadotropin (PMSG) and human chorionic gonadotropin (hCG). On the day of the IVF, the cumulus masses were recovered from the oviducts and incubated with sperm in Cooks medium for 4 hours. The oocytes/zygotes were rinsed in FHM media and incubated in KSOM + amino acids overnight at 37°C. The following day, the number of 2 cell embryos was recorded. Zona Free IVF was performed as before but WT cumulus masses were first incubated in hyaluronidase to remove cumulus cells and zona pellucida (ZP). A sperm-ZP binding assay was performed to observe if Ankef1 KO mouse sperm were able to bind the ZP of WT oocytes. Flagellar waveform analysis was performed using non-capacitated or capacitated spermatozoa from the cauda epididymis of Ankef1+/- and Ankef1-/- males. Spermatozoa were plated on fibronectin-coated coverslips and sperm motility was recorded for 2s with 200 fps on a Zeiss Axioimager Z2M. The initial IVF experiments showed that while fertilization rates remained high for Ankef1+/- sperm, Ankef1-deficient sperm were not able to fertilize WT oocytes. The ZP binding assay showed that Ankef1-/-sperm were able to bind to the ZP, but they were unable to penetrate it, thus fertilization did not occur. Ankef1-/-sperm was not visualized in the perivitelline space in these experiments. However, when the ZP was removed and zona-free IVF performed, fertilization was then restored. Flagellar waveform analysis revealed that Ankef1-/- sperm had a rigid midpiece that rendered their flagella unable to perform the high amplitude bends that define hypermotility and were thus unable to penetrate the ZP. These experiments demonstrate that Ankef1 is required for sperm midpiece flexibility which is required for sperm hyperactivation, and Ankef1 knockout blocks ZP penetration and prevents successful fertilization. Therefore, Ankef1 plays a critical role in sperm function and male fertility." @default.
• W4387396502 created "2023-10-07" @default.
• W4387396502 creator A5004889712 @default.
• W4387396502 creator A5033242458 @default.
• W4387396502 creator A5056043049 @default.
• W4387396502 creator A5063987624 @default.
• W4387396502 creator A5071789149 @default.
• W4387396502 date "2023-10-01" @default.
• W4387396502 modified "2023-10-18" @default.
• W4387396502 title "THE EVOLUTIONARILY CONSERVED PROTEIN ANKEF1 CONFERS SPERM MIDPIECE FLEXIBILITY AND IS ESSENTIAL FOR MALE FERTILITY" @default.
• W4387396502 doi "https://doi.org/10.1016/j.fertnstert.2023.08.416" @default.
• W4387396502 hasPublicationYear "2023" @default.
• W4387396502 type Work @default.
• W4387396502 citedByCount "0" @default.
• W4387396502 crossrefType "journal-article" @default.
• W4387396502 hasAuthorship W4387396502A5004889712 @default.
• W4387396502 hasAuthorship W4387396502A5033242458 @default.
• W4387396502 hasAuthorship W4387396502A5056043049 @default.
• W4387396502 hasAuthorship W4387396502A5063987624 @default.
• W4387396502 hasAuthorship W4387396502A5071789149 @default.
• W4387396502 hasBestOaLocation W43873965021 @default.
• W4387396502 hasConcept C126322002 @default.
• W4387396502 hasConcept C134018914 @default.
• W4387396502 hasConcept C16685009 @default.
• W4387396502 hasConcept C196843134 @default.
• W4387396502 hasConcept C2776010925 @default.
• W4387396502 hasConcept C2776690073 @default.
• W4387396502 hasConcept C2777702977 @default.
• W4387396502 hasConcept C2779234561 @default.
• W4387396502 hasConcept C2780301668 @default.
• W4387396502 hasConcept C2781087480 @default.
• W4387396502 hasConcept C54355233 @default.
• W4387396502 hasConcept C71924100 @default.
• W4387396502 hasConcept C86803240 @default.
• W4387396502 hasConcept C88972607 @default.
• W4387396502 hasConceptScore W4387396502C126322002 @default.
• W4387396502 hasConceptScore W4387396502C134018914 @default.
• W4387396502 hasConceptScore W4387396502C16685009 @default.
• W4387396502 hasConceptScore W4387396502C196843134 @default.
• W4387396502 hasConceptScore W4387396502C2776010925 @default.
• W4387396502 hasConceptScore W4387396502C2776690073 @default.
• W4387396502 hasConceptScore W4387396502C2777702977 @default.
• W4387396502 hasConceptScore W4387396502C2779234561 @default.
• W4387396502 hasConceptScore W4387396502C2780301668 @default.
• W4387396502 hasConceptScore W4387396502C2781087480 @default.
• W4387396502 hasConceptScore W4387396502C54355233 @default.
• W4387396502 hasConceptScore W4387396502C71924100 @default.
• W4387396502 hasConceptScore W4387396502C86803240 @default.
• W4387396502 hasConceptScore W4387396502C88972607 @default.
• W4387396502 hasIssue "4" @default.
• W4387396502 hasLocation W43873965021 @default.
• W4387396502 hasOpenAccess W4387396502 @default.
• W4387396502 hasPrimaryLocation W43873965021 @default.
• W4387396502 hasRelatedWork W2017905860 @default.
• W4387396502 hasRelatedWork W2021575236 @default.
• W4387396502 hasRelatedWork W2073741168 @default.
• W4387396502 hasRelatedWork W2088831817 @default.
• W4387396502 hasRelatedWork W2110563434 @default.
• W4387396502 hasRelatedWork W2152288747 @default.
• W4387396502 hasRelatedWork W2154433224 @default.
• W4387396502 hasRelatedWork W2157088514 @default.
• W4387396502 hasRelatedWork W2368085596 @default.
• W4387396502 hasRelatedWork W2610711863 @default.
• W4387396502 hasVolume "120" @default.
• W4387396502 isParatext "false" @default.
• W4387396502 isRetracted "false" @default.
• W4387396502 workType "article" @default.