FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387396555> ?p ?o ?g. }

• W4387396555 endingPage "e135" @default.
• W4387396555 startingPage "e134" @default.
• W4387396555 abstract "Defective decidualization (DD) of the human endometrium has been associated to reproductive pathologies such as endometriosis or miscarriage of euploid embryos as well as pregnancy complications as severe preeclampsia (sPE). Here, we aim to unveil the global proteome profile underlaying DD of endometrial stromal cells (ESCs) isolated from women who suffered sPE in their previous pregnancy. Endometrial biopsies were collected in the late-secretory phase from women with a previous sPE pregnancy (n=7) and controls with normal pregnancies (n=6). ESCs were isolated and decidualized in vitro using an established protocol (0.5 mM cAMP + 1 μM MPA) in serum-free media during five days. Three technical replicates per sample and condition were included. Cells were lysate and processed for the analysis of intracellular proteins by a single-run liquid chromatography mass spectrometry (LC-MS/MS). Protein quantification was performed using MaxQuant and Perseus software. Immunostaining was performed to validate major findings. A total of 3,947 intracellular proteins were identified in this analysis. The global proteome revealed that ESCs with DD isolated from sPE patients present a differential proteome profile compared controls. The DD group showed significant changes in 272 proteins (FDR < 0.05) and the control group showed significant changes in 263 proteins (FDR < 0.05). The overlapping of the two datasets revealed a common proteome of 133 proteins (∼48%); whereas 52% of proteins composing the global proteome during the decidualization reprogramming was specific for each group. Proteins shared by the two groups (n=133) were involved in biological processes of decidualization such as extracellular matrix, cell migration and tissue remodelling. The proteins specific of controls (n=130) were also involved in pathways of decidualization biology, while the proteins specific of sPE (n=139) revealed an enrichment for pathways involved in response to viral infections such as entry into host cell. Notably, ESCs from sPE show upregulated proteins involved in inflammasomes inhibition. Inflammasomes are a complex of proteins that induce immune responses to pathogens, and they have been associated with placental inflammation in sPE pregnancies. We assessed two classical markers of inflammasomes (ASC and IL-1B) in vivo by immunofluorescence, corroborating the downregulation in DD. ESCs isolated from sPE patients show an abnormal proteome profile in response to hormonal stimuli leading to DD. This proteomic footprint includes proteins involved in altered decidualization pathways, but also proteins involved in response to viral infections. Further, our data evidence an alteration of inflammasome machinery during DD in sPE." @default.
• W4387396555 created "2023-10-07" @default.
• W4387396555 creator A5003851931 @default.
• W4387396555 creator A5012781227 @default.
• W4387396555 creator A5021163172 @default.
• W4387396555 creator A5047224915 @default.
• W4387396555 creator A5048033089 @default.
• W4387396555 creator A5067487196 @default.
• W4387396555 creator A5073970595 @default.
• W4387396555 creator A5090345459 @default.
• W4387396555 creator A5093015442 @default.
• W4387396555 date "2023-10-01" @default.
• W4387396555 modified "2023-10-07" @default.
• W4387396555 title "GLOBAL PROTEOME PROFILE OF DEFECTIVE DECIDUALIZATION" @default.
• W4387396555 doi "https://doi.org/10.1016/j.fertnstert.2023.08.417" @default.
• W4387396555 hasPublicationYear "2023" @default.
• W4387396555 type Work @default.
• W4387396555 citedByCount "0" @default.
• W4387396555 crossrefType "journal-article" @default.
• W4387396555 hasAuthorship W4387396555A5003851931 @default.
• W4387396555 hasAuthorship W4387396555A5012781227 @default.
• W4387396555 hasAuthorship W4387396555A5021163172 @default.
• W4387396555 hasAuthorship W4387396555A5047224915 @default.
• W4387396555 hasAuthorship W4387396555A5048033089 @default.
• W4387396555 hasAuthorship W4387396555A5067487196 @default.
• W4387396555 hasAuthorship W4387396555A5073970595 @default.
• W4387396555 hasAuthorship W4387396555A5090345459 @default.
• W4387396555 hasAuthorship W4387396555A5093015442 @default.
• W4387396555 hasBestOaLocation W43873965551 @default.
• W4387396555 hasConcept C104317684 @default.
• W4387396555 hasConcept C104397665 @default.
• W4387396555 hasConcept C126322002 @default.
• W4387396555 hasConcept C134018914 @default.
• W4387396555 hasConcept C16685009 @default.
• W4387396555 hasConcept C16930146 @default.
• W4387396555 hasConcept C196843134 @default.
• W4387396555 hasConcept C203014093 @default.
• W4387396555 hasConcept C2778316770 @default.
• W4387396555 hasConcept C2779234561 @default.
• W4387396555 hasConcept C2779742232 @default.
• W4387396555 hasConcept C46111723 @default.
• W4387396555 hasConcept C502942594 @default.
• W4387396555 hasConcept C54355233 @default.
• W4387396555 hasConcept C55493867 @default.
• W4387396555 hasConcept C60644358 @default.
• W4387396555 hasConcept C71924100 @default.
• W4387396555 hasConcept C86803240 @default.
• W4387396555 hasConcept C95444343 @default.
• W4387396555 hasConceptScore W4387396555C104317684 @default.
• W4387396555 hasConceptScore W4387396555C104397665 @default.
• W4387396555 hasConceptScore W4387396555C126322002 @default.
• W4387396555 hasConceptScore W4387396555C134018914 @default.
• W4387396555 hasConceptScore W4387396555C16685009 @default.
• W4387396555 hasConceptScore W4387396555C16930146 @default.
• W4387396555 hasConceptScore W4387396555C196843134 @default.
• W4387396555 hasConceptScore W4387396555C203014093 @default.
• W4387396555 hasConceptScore W4387396555C2778316770 @default.
• W4387396555 hasConceptScore W4387396555C2779234561 @default.
• W4387396555 hasConceptScore W4387396555C2779742232 @default.
• W4387396555 hasConceptScore W4387396555C46111723 @default.
• W4387396555 hasConceptScore W4387396555C502942594 @default.
• W4387396555 hasConceptScore W4387396555C54355233 @default.
• W4387396555 hasConceptScore W4387396555C55493867 @default.
• W4387396555 hasConceptScore W4387396555C60644358 @default.
• W4387396555 hasConceptScore W4387396555C71924100 @default.
• W4387396555 hasConceptScore W4387396555C86803240 @default.
• W4387396555 hasConceptScore W4387396555C95444343 @default.
• W4387396555 hasIssue "4" @default.
• W4387396555 hasLocation W43873965551 @default.
• W4387396555 hasOpenAccess W4387396555 @default.
• W4387396555 hasPrimaryLocation W43873965551 @default.
• W4387396555 hasRelatedWork W1030154654 @default.
• W4387396555 hasRelatedWork W1998259517 @default.
• W4387396555 hasRelatedWork W2137657922 @default.
• W4387396555 hasRelatedWork W2171821489 @default.
• W4387396555 hasRelatedWork W2358522016 @default.
• W4387396555 hasRelatedWork W2397127137 @default.
• W4387396555 hasRelatedWork W2592509224 @default.
• W4387396555 hasRelatedWork W2738975990 @default.
• W4387396555 hasRelatedWork W4205777856 @default.
• W4387396555 hasRelatedWork W4235382972 @default.
• W4387396555 hasVolume "120" @default.
• W4387396555 isParatext "false" @default.
• W4387396555 isRetracted "false" @default.
• W4387396555 workType "article" @default.