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- W4387402854 abstract "Identification of plasmids from sequencing data is an important and challenging problem related to antimicrobial resistance spread and other One-Health issues. We provide a new architecture for identifying plasmid contigs in fragmented genome assemblies built from short-read data. We employ graph neural networks (GNNs) and the assembly graph to propagate the information from nearby nodes, which leads to more accurate classification, especially for short contigs that are difficult to classify based on sequence features or database searches alone. We trained plASgraph2 on a data set of samples from the ESKAPEE group of pathogens. plASgraph2 either outperforms or performs on par with a wide range of state-of-the-art methods on testing sets of independent ESKAPEE samples and samples from related pathogens. On one hand, our study provides a new accurate and easy to use tool for contig classification in bacterial isolates; on the other hand, it serves as a proof-of-concept for the use of GNNs in genomics. Our software is available at https://github.com/cchauve/plasgraph2 and the training and testing data sets are available at https://github.com/fmfi-compbio/plasgraph2-datasets ." @default.
- W4387402854 created "2023-10-07" @default.
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- W4387402854 date "2023-10-06" @default.
- W4387402854 modified "2023-10-07" @default.
- W4387402854 title "plASgraph2: using graph neural networks to detect plasmid contigs from an assembly graph" @default.
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- W4387402854 doi "https://doi.org/10.3389/fmicb.2023.1267695" @default.
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