FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387403734> ?p ?o ?g. }

• W4387403734 endingPage "e10" @default.
• W4387403734 startingPage "e10" @default.
• W4387403734 abstract "BRCA1 and 2 are the most common genetic mutations in hereditary breast cancer. Women with BRCA1 and 2 mutations have been shown to have a diminished primordial follicle density and increased oocyte DNA damage, but studies did not differentiate between BRCA1 and 2. Our objectives include: 1. Determine primordial and primary follicle density in BRCA1 and BRCA2 carriers compared to age matched controls. 2. Identify markers of DNA damage in oocytes of BRCA1 and BRCA2 carriers compared to age matched controls. We performed a retrospective case-control study using ovarian tissue from patients age 18-40 with a BRCA1 or 2 mutation who underwent risk-reducing bilateral salpingo-oophorectomy. Samples from age matched controls who underwent unilateral or bilateral oophorectomies were collected. Exclusion criteria included ovarian cancer, prior chemotherapy or radiation, ovarian pathology including endometriosis, or postmenopausal status. Multiple Formalin-fixed and paraffin-embedded tissue blocks were sectioned and stained with hematoxylin and eosin and follicles counted by two blinded counters. Immunohistochemical analysis of phosphorylated histone H2AX (γH2AX) and phospho-ATM (p-ATM) were used to assess DNA double strand breaks. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay for oocyte apoptosis was performed. Statistical analysis was performed in Graphpad Prism. One way ANOVA with Tukey’s multiple comparisons test was used for all tests except for follicle density which was analyzed with two-way ANOVA with Tukey’s multiple comparisons test. A total of 30 subjects were included: 8 BRCA1, 9 BRCA2, and 13 controls. There were no significant differences in mean age. Only BRCA1 carriers had a statistically significant decrease in primordial follicle density compared to controls (p=0.036). Neither BRCA group had altered primary follicle density compared to controls. BRCA1 carriers had a statistically higher percentage of p-ATM positivity compared to controls (63.3% vs 42.6%, p=0.134). BRCA2 carriers had a higher p-ATM positivity compared to controls but it was not statistically significant (57.9% vs 42.6%, p=0.066). Both BRCA groups had statistically significant higher positivity for γH2AX than controls (52.9% vs 31.0%, p=0.0002; 50.3% vs 31.0% p=0.0005). Both BRCA groups had higher levels of TUNEL positivity than controls, but it was not statistically significant (45.4% vs 32.8%, p=0.149; 46.2% vs 32.8%, p=0.105). BRCA1 carriers have significantly lower primordial follicle density and higher levels of oocyte DNA damage. Though BRCA2 carriers do not have a decrease in follicle density, they do have significantly higher levels of DNA damage." @default.
• W4387403734 created "2023-10-07" @default.
• W4387403734 creator A5003330575 @default.
• W4387403734 creator A5027601058 @default.
• W4387403734 creator A5035866527 @default.
• W4387403734 creator A5036190843 @default.
• W4387403734 creator A5040070446 @default.
• W4387403734 creator A5042783698 @default.
• W4387403734 creator A5043369822 @default.
• W4387403734 creator A5047178033 @default.
• W4387403734 creator A5047621200 @default.
• W4387403734 creator A5069271756 @default.
• W4387403734 creator A5080157873 @default.
• W4387403734 creator A5089191061 @default.
• W4387403734 creator A5093017239 @default.
• W4387403734 date "2023-10-01" @default.
• W4387403734 modified "2023-10-07" @default.
• W4387403734 title "THE EFFECTS OF THE BRCA1 AND 2 MUTATIONS ON OVARIAN RESERVE AND AGING" @default.
• W4387403734 doi "https://doi.org/10.1016/j.fertnstert.2023.08.083" @default.
• W4387403734 hasPublicationYear "2023" @default.
• W4387403734 type Work @default.
• W4387403734 citedByCount "0" @default.
• W4387403734 crossrefType "journal-article" @default.
• W4387403734 hasAuthorship W4387403734A5003330575 @default.
• W4387403734 hasAuthorship W4387403734A5027601058 @default.
• W4387403734 hasAuthorship W4387403734A5035866527 @default.
• W4387403734 hasAuthorship W4387403734A5036190843 @default.
• W4387403734 hasAuthorship W4387403734A5040070446 @default.
• W4387403734 hasAuthorship W4387403734A5042783698 @default.
• W4387403734 hasAuthorship W4387403734A5043369822 @default.
• W4387403734 hasAuthorship W4387403734A5047178033 @default.
• W4387403734 hasAuthorship W4387403734A5047621200 @default.
• W4387403734 hasAuthorship W4387403734A5069271756 @default.
• W4387403734 hasAuthorship W4387403734A5080157873 @default.
• W4387403734 hasAuthorship W4387403734A5089191061 @default.
• W4387403734 hasAuthorship W4387403734A5093017239 @default.
• W4387403734 hasBestOaLocation W43874037341 @default.
• W4387403734 hasConcept C121608353 @default.
• W4387403734 hasConcept C126322002 @default.
• W4387403734 hasConcept C134018914 @default.
• W4387403734 hasConcept C142724271 @default.
• W4387403734 hasConcept C143425029 @default.
• W4387403734 hasConcept C143998085 @default.
• W4387403734 hasConcept C16685009 @default.
• W4387403734 hasConcept C196843134 @default.
• W4387403734 hasConcept C2776600139 @default.
• W4387403734 hasConcept C2776659692 @default.
• W4387403734 hasConcept C2776690073 @default.
• W4387403734 hasConcept C2777688143 @default.
• W4387403734 hasConcept C2778324911 @default.
• W4387403734 hasConcept C2779234561 @default.
• W4387403734 hasConcept C2780427987 @default.
• W4387403734 hasConcept C2780536345 @default.
• W4387403734 hasConcept C54355233 @default.
• W4387403734 hasConcept C5512455 @default.
• W4387403734 hasConcept C552990157 @default.
• W4387403734 hasConcept C71924100 @default.
• W4387403734 hasConcept C86803240 @default.
• W4387403734 hasConcept C99476002 @default.
• W4387403734 hasConceptScore W4387403734C121608353 @default.
• W4387403734 hasConceptScore W4387403734C126322002 @default.
• W4387403734 hasConceptScore W4387403734C134018914 @default.
• W4387403734 hasConceptScore W4387403734C142724271 @default.
• W4387403734 hasConceptScore W4387403734C143425029 @default.
• W4387403734 hasConceptScore W4387403734C143998085 @default.
• W4387403734 hasConceptScore W4387403734C16685009 @default.
• W4387403734 hasConceptScore W4387403734C196843134 @default.
• W4387403734 hasConceptScore W4387403734C2776600139 @default.
• W4387403734 hasConceptScore W4387403734C2776659692 @default.
• W4387403734 hasConceptScore W4387403734C2776690073 @default.
• W4387403734 hasConceptScore W4387403734C2777688143 @default.
• W4387403734 hasConceptScore W4387403734C2778324911 @default.
• W4387403734 hasConceptScore W4387403734C2779234561 @default.
• W4387403734 hasConceptScore W4387403734C2780427987 @default.
• W4387403734 hasConceptScore W4387403734C2780536345 @default.
• W4387403734 hasConceptScore W4387403734C54355233 @default.
• W4387403734 hasConceptScore W4387403734C5512455 @default.
• W4387403734 hasConceptScore W4387403734C552990157 @default.
• W4387403734 hasConceptScore W4387403734C71924100 @default.
• W4387403734 hasConceptScore W4387403734C86803240 @default.
• W4387403734 hasConceptScore W4387403734C99476002 @default.
• W4387403734 hasIssue "4" @default.
• W4387403734 hasLocation W43874037341 @default.
• W4387403734 hasOpenAccess W4387403734 @default.
• W4387403734 hasPrimaryLocation W43874037341 @default.
• W4387403734 hasRelatedWork W2042163600 @default.
• W4387403734 hasRelatedWork W2109177107 @default.
• W4387403734 hasRelatedWork W2124108876 @default.
• W4387403734 hasRelatedWork W2131693513 @default.
• W4387403734 hasRelatedWork W2507175452 @default.
• W4387403734 hasRelatedWork W2582733603 @default.
• W4387403734 hasRelatedWork W2592976810 @default.
• W4387403734 hasRelatedWork W3170532129 @default.
• W4387403734 hasRelatedWork W4296685787 @default.
• W4387403734 hasRelatedWork W4381686728 @default.
• W4387403734 hasVolume "120" @default.
• W4387403734 isParatext "false" @default.
• W4387403734 isRetracted "false" @default.

• next