FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387406194> ?p ?o ?g. }

• W4387406194 abstract "The mechanisms and regulation for DNA replication in plant organelles are largely unknown, as few proteins involved in replisome assembly have been biochemically studied. A primase-helicase dubbed Twinkle (T7 gp4-like protein with intramitochondrial nucleoid localization) unwinds double-stranded DNA in metazoan mitochondria and plant organelles. Twinkle in plants is a bifunctional enzyme with an active primase module. This contrast with animal Twinkle in which the primase module is inactive. The organellar primase-helicase of Arabidopsis thaliana (AtTwinkle) harbors a primase module (AtPrimase) that consists of an RNA polymerase domain (RPD) and a Zn + + finger domain (ZFD).Herein, we investigate the mechanisms by which AtTwinkle recognizes its templating sequence and how primer synthesis and coupling to the organellar DNA polymerases occurs. Biochemical data show that the ZFD of the AtPrimase module is responsible for template recognition, and this recognition is achieved by residues N163, R166, and K168. The role of the ZFD in template recognition was also corroborated by swapping the RPDs of bacteriophage T7 primase and AtPrimase with their respective ZFDs. A chimeric primase harboring the ZFD of T7 primase and the RPD of AtPrimase synthesizes ribonucleotides from the T7 primase recognition sequence and conversely, a chimeric primase harboring the ZFD of AtPrimase and the RPD of T7 primase synthesizes ribonucleotides from the AtPrimase recognition sequence. A chimera harboring the RPDs of bacteriophage T7 and the ZBD of AtTwinkle efficiently synthesizes primers for the plant organellar DNA polymerase.We conclude that the ZFD is responsible for recognizing a single-stranded sequence and for primer hand-off into the organellar DNA polymerases active site. The primase activity of plant Twinkle is consistent with phylogeny-based reconstructions that concluded that Twinkle´s last eukaryotic common ancestor (LECA) was an enzyme with primase and helicase activities. In plants, the primase domain is active, whereas the primase activity was lost in metazoans. Our data supports the notion that AtTwinkle synthesizes primers at the lagging-strand of the organellar replication fork." @default.
• W4387406194 created "2023-10-07" @default.
• W4387406194 creator A5002433394 @default.
• W4387406194 creator A5016300314 @default.
• W4387406194 creator A5058451525 @default.
• W4387406194 creator A5060584050 @default.
• W4387406194 creator A5070278817 @default.
• W4387406194 creator A5087397158 @default.
• W4387406194 date "2023-10-06" @default.
• W4387406194 modified "2023-10-11" @default.
• W4387406194 title "The plant organellar primase-helicase directs template recognition and primosome assembly via its zinc finger domain" @default.
• W4387406194 cites W1546157935 @default.
• W4387406194 cites W1970571754 @default.
• W4387406194 cites W1971511990 @default.
• W4387406194 cites W1975910300 @default.
• W4387406194 cites W1984314142 @default.
• W4387406194 cites W1985907558 @default.
• W4387406194 cites W1994390368 @default.
• W4387406194 cites W1998036609 @default.
• W4387406194 cites W1998129350 @default.
• W4387406194 cites W2019069746 @default.
• W4387406194 cites W2020885690 @default.
• W4387406194 cites W2022805241 @default.
• W4387406194 cites W2038994322 @default.
• W4387406194 cites W2053995121 @default.
• W4387406194 cites W2055516948 @default.
• W4387406194 cites W2057091844 @default.
• W4387406194 cites W2060512149 @default.
• W4387406194 cites W2072186902 @default.
• W4387406194 cites W2084008778 @default.
• W4387406194 cites W2088593314 @default.
• W4387406194 cites W2093122853 @default.
• W4387406194 cites W2145723200 @default.
• W4387406194 cites W2147821579 @default.
• W4387406194 cites W2161569491 @default.
• W4387406194 cites W2162880055 @default.
• W4387406194 cites W2196379590 @default.
• W4387406194 cites W2196646621 @default.
• W4387406194 cites W2408638299 @default.
• W4387406194 cites W2468770811 @default.
• W4387406194 cites W2515929849 @default.
• W4387406194 cites W2554096736 @default.
• W4387406194 cites W2750957757 @default.
• W4387406194 cites W2753883028 @default.
• W4387406194 cites W2764061625 @default.
• W4387406194 cites W2770009792 @default.
• W4387406194 cites W2901579467 @default.
• W4387406194 cites W2911493788 @default.
• W4387406194 cites W2912332641 @default.
• W4387406194 cites W2948928478 @default.
• W4387406194 cites W2990556781 @default.
• W4387406194 cites W3016129918 @default.
• W4387406194 cites W3170693367 @default.
• W4387406194 cites W3174148415 @default.
• W4387406194 cites W3205378053 @default.
• W4387406194 cites W4229608773 @default.
• W4387406194 cites W4296818351 @default.
• W4387406194 cites W4366237427 @default.
• W4387406194 doi "https://doi.org/10.1186/s12870-023-04477-4" @default.
• W4387406194 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37803262" @default.
• W4387406194 hasPublicationYear "2023" @default.
• W4387406194 type Work @default.
• W4387406194 citedByCount "0" @default.
• W4387406194 crossrefType "journal-article" @default.
• W4387406194 hasAuthorship W4387406194A5002433394 @default.
• W4387406194 hasAuthorship W4387406194A5016300314 @default.
• W4387406194 hasAuthorship W4387406194A5058451525 @default.
• W4387406194 hasAuthorship W4387406194A5060584050 @default.
• W4387406194 hasAuthorship W4387406194A5070278817 @default.
• W4387406194 hasAuthorship W4387406194A5087397158 @default.
• W4387406194 hasBestOaLocation W43874061941 @default.
• W4387406194 hasConcept C104317684 @default.
• W4387406194 hasConcept C149187182 @default.
• W4387406194 hasConcept C153911025 @default.
• W4387406194 hasConcept C156719811 @default.
• W4387406194 hasConcept C161223559 @default.
• W4387406194 hasConcept C194830204 @default.
• W4387406194 hasConcept C2756471 @default.
• W4387406194 hasConcept C54355233 @default.
• W4387406194 hasConcept C552990157 @default.
• W4387406194 hasConcept C55493867 @default.
• W4387406194 hasConcept C58167604 @default.
• W4387406194 hasConcept C67705224 @default.
• W4387406194 hasConcept C73573662 @default.
• W4387406194 hasConcept C86803240 @default.
• W4387406194 hasConcept C95444343 @default.
• W4387406194 hasConceptScore W4387406194C104317684 @default.
• W4387406194 hasConceptScore W4387406194C149187182 @default.
• W4387406194 hasConceptScore W4387406194C153911025 @default.
• W4387406194 hasConceptScore W4387406194C156719811 @default.
• W4387406194 hasConceptScore W4387406194C161223559 @default.
• W4387406194 hasConceptScore W4387406194C194830204 @default.
• W4387406194 hasConceptScore W4387406194C2756471 @default.
• W4387406194 hasConceptScore W4387406194C54355233 @default.
• W4387406194 hasConceptScore W4387406194C552990157 @default.
• W4387406194 hasConceptScore W4387406194C55493867 @default.
• W4387406194 hasConceptScore W4387406194C58167604 @default.
• W4387406194 hasConceptScore W4387406194C67705224 @default.
• W4387406194 hasConceptScore W4387406194C73573662 @default.
• W4387406194 hasConceptScore W4387406194C86803240 @default.

• next