Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387431566> ?p ?o ?g. }
- W4387431566 endingPage "106377" @default.
- W4387431566 startingPage "106377" @default.
- W4387431566 abstract "Mycobacterium tuberculosis (Mtb) is a crucial and highly destructive intracellular pathogen responsible for causing tuberculosis (TB). The emergence and dissemination of multi-drug resistant Mtb has further aggravated the TB crisis, leading to high mortality. Mtb FadD2 is a fatty acyl-coenzyme A (CoA) synthetase that modifies the cell envelope and plays an important role in reducing Mtb susceptibility to pyrazinoic acid (POA). However, the functional mechanism of Mtb FadD2 remains poorly understood. Here, we successfully expressed, purified and obtained monomeric FadD2 by using buffer (500 mM NaCl, 20 mM Tris-HCl, pH7.4 and 5 % glycerol). Palmitate was found to be the optimal substrate for FadD2. Fatty acyl-CoA synthetase activity reached maximum at 450 μM palmitate, and the Km value was 318.2 μM for palmitate. The results of mutation experiments indicated the critical role of T370 and K551 in the enzymatic activity of FadD2. Our work provides a guideline and concept for the development of novel drugs against Mtb." @default.
- W4387431566 created "2023-10-08" @default.
- W4387431566 creator A5025110808 @default.
- W4387431566 creator A5035644244 @default.
- W4387431566 creator A5039313839 @default.
- W4387431566 creator A5046059077 @default.
- W4387431566 creator A5047569518 @default.
- W4387431566 date "2023-10-01" @default.
- W4387431566 modified "2023-10-11" @default.
- W4387431566 title "Recombinant expression and functional characterization of FadD2 protein in Mycobacterium tuberculosis" @default.
- W4387431566 cites W1489667240 @default.
- W4387431566 cites W1979746007 @default.
- W4387431566 cites W1980776509 @default.
- W4387431566 cites W2065903108 @default.
- W4387431566 cites W2084092304 @default.
- W4387431566 cites W2100383399 @default.
- W4387431566 cites W2105668062 @default.
- W4387431566 cites W2115993724 @default.
- W4387431566 cites W2120651372 @default.
- W4387431566 cites W2129047823 @default.
- W4387431566 cites W2136776926 @default.
- W4387431566 cites W2140564701 @default.
- W4387431566 cites W2141711680 @default.
- W4387431566 cites W2158023121 @default.
- W4387431566 cites W2166113903 @default.
- W4387431566 cites W2171815113 @default.
- W4387431566 cites W2466404575 @default.
- W4387431566 cites W2551306185 @default.
- W4387431566 cites W2600677235 @default.
- W4387431566 cites W2602169678 @default.
- W4387431566 cites W2792154364 @default.
- W4387431566 cites W2925159988 @default.
- W4387431566 cites W2973028036 @default.
- W4387431566 cites W3034731016 @default.
- W4387431566 cites W3127646014 @default.
- W4387431566 cites W3183475563 @default.
- W4387431566 cites W3204813878 @default.
- W4387431566 cites W4200033378 @default.
- W4387431566 cites W4211157605 @default.
- W4387431566 cites W4214719116 @default.
- W4387431566 cites W4214894533 @default.
- W4387431566 cites W4220836245 @default.
- W4387431566 cites W4225390945 @default.
- W4387431566 cites W4229076896 @default.
- W4387431566 cites W4237057354 @default.
- W4387431566 cites W4254419430 @default.
- W4387431566 cites W4293109488 @default.
- W4387431566 cites W4318192424 @default.
- W4387431566 cites W4322622274 @default.
- W4387431566 cites W4323047550 @default.
- W4387431566 cites W4365787616 @default.
- W4387431566 doi "https://doi.org/10.1016/j.pep.2023.106377" @default.
- W4387431566 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37813293" @default.
- W4387431566 hasPublicationYear "2023" @default.
- W4387431566 type Work @default.
- W4387431566 citedByCount "0" @default.
- W4387431566 crossrefType "journal-article" @default.
- W4387431566 hasAuthorship W4387431566A5025110808 @default.
- W4387431566 hasAuthorship W4387431566A5035644244 @default.
- W4387431566 hasAuthorship W4387431566A5039313839 @default.
- W4387431566 hasAuthorship W4387431566A5046059077 @default.
- W4387431566 hasAuthorship W4387431566A5047569518 @default.
- W4387431566 hasConcept C104317684 @default.
- W4387431566 hasConcept C108305142 @default.
- W4387431566 hasConcept C134651460 @default.
- W4387431566 hasConcept C142724271 @default.
- W4387431566 hasConcept C181199279 @default.
- W4387431566 hasConcept C185592680 @default.
- W4387431566 hasConcept C2777975735 @default.
- W4387431566 hasConcept C2779123261 @default.
- W4387431566 hasConcept C2781069245 @default.
- W4387431566 hasConcept C40767141 @default.
- W4387431566 hasConcept C55493867 @default.
- W4387431566 hasConcept C71924100 @default.
- W4387431566 hasConcept C79879829 @default.
- W4387431566 hasConcept C86803240 @default.
- W4387431566 hasConcept C89423630 @default.
- W4387431566 hasConceptScore W4387431566C104317684 @default.
- W4387431566 hasConceptScore W4387431566C108305142 @default.
- W4387431566 hasConceptScore W4387431566C134651460 @default.
- W4387431566 hasConceptScore W4387431566C142724271 @default.
- W4387431566 hasConceptScore W4387431566C181199279 @default.
- W4387431566 hasConceptScore W4387431566C185592680 @default.
- W4387431566 hasConceptScore W4387431566C2777975735 @default.
- W4387431566 hasConceptScore W4387431566C2779123261 @default.
- W4387431566 hasConceptScore W4387431566C2781069245 @default.
- W4387431566 hasConceptScore W4387431566C40767141 @default.
- W4387431566 hasConceptScore W4387431566C55493867 @default.
- W4387431566 hasConceptScore W4387431566C71924100 @default.
- W4387431566 hasConceptScore W4387431566C79879829 @default.
- W4387431566 hasConceptScore W4387431566C86803240 @default.
- W4387431566 hasConceptScore W4387431566C89423630 @default.
- W4387431566 hasFunder F4320321001 @default.
- W4387431566 hasFunder F4320335777 @default.
- W4387431566 hasLocation W43874315661 @default.
- W4387431566 hasLocation W43874315662 @default.
- W4387431566 hasOpenAccess W4387431566 @default.
- W4387431566 hasPrimaryLocation W43874315661 @default.