FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387432918> ?p ?o ?g. }

• W4387432918 abstract "SUMMARY The unsaturation of phospholipids influences the function of membranes. In Arabidopsis thaliana , the oleoyl Δ12‐desaturase FAD2 converts oleic (18:1 Δ9 ) to linoleic acid (18:2 Δ9,12 ) and influences phospholipid unsaturation in different cellular membranes. Despite its importance, the precise localization of Arabidopsis FAD2 has not been unambiguously described. As FAD2 is thought to modify phospholipid‐associated fatty acids at the endoplasmic reticulum (ER), from where unsaturates are distributed to other cellular sites, we hypothesized that FAD2 locates to ER subdomains enabling trafficking of lipid intermediates through the secretory pathway. Fluorescent FAD2 fusions used to test this hypothesis were first assessed for functionality by heterologous expression in yeast ( Saccharomyces cerevisiae ), and in planta by Arabidopsis fad2 mutant rescue upon ectopic expression from an intrinsic FAD2 promoter fragment. Light sheet fluorescence, laser scanning confocal or spinning disc microscopy of roots, leaves, or mesophyll protoplasts showed the functional fluorescence‐tagged FAD2 variants in flattened donut‐shaped structures of ~0.5–1 μm diameter, in a pattern not resembling mere ER association. High‐resolution imaging of coexpressed organellar markers showed fluorescence‐tagged FAD2 in a ring‐shaped pattern surrounding ER‐proximal Golgi particles, colocalizing with pre‐ cis ‐Golgi markers. This localization required the unusual C‐terminal retention signal of FAD2, and deletion or substitutions in this protein region resulted in relaxed distribution and diffuse association with the ER. The distinct association of FAD2 with pre‐ cis ‐Golgi stacks in Arabidopsis root and leaf tissue is consistent with a contribution of FAD2 to membrane lipid homeostasis through the secretory pathway, as verified by an increased plasma membrane liquid phase order in the fad2 mutant." @default.
• W4387432918 created "2023-10-09" @default.
• W4387432918 creator A5000313519 @default.
• W4387432918 creator A5013063715 @default.
• W4387432918 creator A5019190387 @default.
• W4387432918 creator A5029338354 @default.
• W4387432918 creator A5066584406 @default.
• W4387432918 creator A5090211870 @default.
• W4387432918 creator A5093023371 @default.
• W4387432918 date "2023-10-08" @default.
• W4387432918 modified "2023-10-11" @default.
• W4387432918 title "Association of the Arabidopsis oleoyl <scp>Δ12</scp>‐desaturase <scp>FAD2</scp> with <scp>pre‐</scp><i>cis</i><scp>‐Golgi</scp> stacks at <scp>endoplasmic reticulum‐Golgi</scp>‐exit sites" @default.
• W4387432918 cites W1539018267 @default.
• W4387432918 cites W1571326346 @default.
• W4387432918 cites W1573563376 @default.
• W4387432918 cites W1966884806 @default.
• W4387432918 cites W1973782344 @default.
• W4387432918 cites W1981552494 @default.
• W4387432918 cites W1989109435 @default.
• W4387432918 cites W1989603573 @default.
• W4387432918 cites W1997317410 @default.
• W4387432918 cites W1997980576 @default.
• W4387432918 cites W1998342535 @default.
• W4387432918 cites W2016181161 @default.
• W4387432918 cites W2016993318 @default.
• W4387432918 cites W2025078829 @default.
• W4387432918 cites W2044952220 @default.
• W4387432918 cites W2052140378 @default.
• W4387432918 cites W2052821301 @default.
• W4387432918 cites W2067117707 @default.
• W4387432918 cites W2076324120 @default.
• W4387432918 cites W2077005028 @default.
• W4387432918 cites W2083868507 @default.
• W4387432918 cites W2088875625 @default.
• W4387432918 cites W2098435260 @default.
• W4387432918 cites W2100738466 @default.
• W4387432918 cites W2101894478 @default.
• W4387432918 cites W2103908409 @default.
• W4387432918 cites W2104609417 @default.
• W4387432918 cites W2107247647 @default.
• W4387432918 cites W2109944792 @default.
• W4387432918 cites W2116122104 @default.
• W4387432918 cites W2124733101 @default.
• W4387432918 cites W2135487705 @default.
• W4387432918 cites W2148397172 @default.
• W4387432918 cites W2148963859 @default.
• W4387432918 cites W2153168072 @default.
• W4387432918 cites W2164251676 @default.
• W4387432918 cites W2167279371 @default.
• W4387432918 cites W2300552860 @default.
• W4387432918 cites W2936237704 @default.
• W4387432918 cites W2960888837 @default.
• W4387432918 cites W2967717096 @default.
• W4387432918 cites W2991029173 @default.
• W4387432918 cites W3045394216 @default.
• W4387432918 cites W3083635513 @default.
• W4387432918 cites W3131644197 @default.
• W4387432918 cites W3165469069 @default.
• W4387432918 cites W4303980945 @default.
• W4387432918 doi "https://doi.org/10.1111/tpj.16492" @default.
• W4387432918 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37805827" @default.
• W4387432918 hasPublicationYear "2023" @default.
• W4387432918 type Work @default.
• W4387432918 citedByCount "0" @default.
• W4387432918 crossrefType "journal-article" @default.
• W4387432918 hasAuthorship W4387432918A5000313519 @default.
• W4387432918 hasAuthorship W4387432918A5013063715 @default.
• W4387432918 hasAuthorship W4387432918A5019190387 @default.
• W4387432918 hasAuthorship W4387432918A5029338354 @default.
• W4387432918 hasAuthorship W4387432918A5066584406 @default.
• W4387432918 hasAuthorship W4387432918A5090211870 @default.
• W4387432918 hasAuthorship W4387432918A5093023371 @default.
• W4387432918 hasBestOaLocation W43874329181 @default.
• W4387432918 hasConcept C104317684 @default.
• W4387432918 hasConcept C119062480 @default.
• W4387432918 hasConcept C142613039 @default.
• W4387432918 hasConcept C143065580 @default.
• W4387432918 hasConcept C158617107 @default.
• W4387432918 hasConcept C19038510 @default.
• W4387432918 hasConcept C2775988993 @default.
• W4387432918 hasConcept C2777129432 @default.
• W4387432918 hasConcept C2777576037 @default.
• W4387432918 hasConcept C2778918659 @default.
• W4387432918 hasConcept C2779222958 @default.
• W4387432918 hasConcept C2779491563 @default.
• W4387432918 hasConcept C2780809830 @default.
• W4387432918 hasConcept C41625074 @default.
• W4387432918 hasConcept C543025807 @default.
• W4387432918 hasConcept C55493867 @default.
• W4387432918 hasConcept C86803240 @default.
• W4387432918 hasConcept C95444343 @default.
• W4387432918 hasConceptScore W4387432918C104317684 @default.
• W4387432918 hasConceptScore W4387432918C119062480 @default.
• W4387432918 hasConceptScore W4387432918C142613039 @default.
• W4387432918 hasConceptScore W4387432918C143065580 @default.
• W4387432918 hasConceptScore W4387432918C158617107 @default.
• W4387432918 hasConceptScore W4387432918C19038510 @default.
• W4387432918 hasConceptScore W4387432918C2775988993 @default.
• W4387432918 hasConceptScore W4387432918C2777129432 @default.
• W4387432918 hasConceptScore W4387432918C2777576037 @default.

• next