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• W4387447918 abstract "The TWEAK receptor, Fn14, is a promising candidate for active targeting of cancer nanotherapeutics to many solid tumor types, including metastatic breast and primary brain cancers. Targeting of therapeutic nanoparticles (NPs) has been accomplished using a range of targeting moieties including monoclonal antibodies and related fragments, peptides, and small molecules. Here, we investigated a full-length Fn14-specific monoclonal antibody, ITEM4, or an ITEM4-Fab fragment as a targeting moiety to guide the development of a clinical formulation. We formulated NPs with varying densities of the targeting moieties while maintaining the decreased nonspecific adhesivity with receptor targeting (DART) characteristics. To model the conditions that NPs experience following intravenous infusion, we investigated the impact of serum exposure in relation to the targeting moiety type and surface density. To further evaluate performance at the cancer cell level, we performed experiments to assess differences in cellular uptake and trafficking in several cancer cell lines using confocal microscopy, imaging flow cytometry, and total internal reflection fluorescence microscopy. We observed that Fn14-targeted NPs exhibit enhanced cellular uptake in Fn14-high compared to Fn14-low cancer cells and that in both cell lines uptake levels were greater than observed with control, nontargeted NPs. We found that serum exposure increased Fn14-targeted NP specificity while simultaneously reducing the total NP uptake. Importantly, serum exposure caused a larger reduction in cancer cell uptake over time when the targeting moiety was an antibody fragment (Fab region of the monoclonal antibody) compared with the full-length monoclonal antibody targeting moiety. Lastly, we uncovered that full monoclonal antibody-targeted NPs enter cancer cells via clathrin-mediated endocytosis and traffic through the endolysosomal pathway. Taken together, these results support a pathway for developing a clinical formulation using a full-length Fn14 monoclonal antibody as the targeting moiety for a DART cancer nanotherapeutic agent." @default.
• W4387447918 created "2023-10-10" @default.
• W4387447918 creator A5003969438 @default.
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• W4387447918 creator A5030867486 @default.
• W4387447918 creator A5032029297 @default.
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• W4387447918 creator A5044528229 @default.
• W4387447918 creator A5070654931 @default.
• W4387447918 creator A5088403801 @default.
• W4387447918 date "2023-10-09" @default.
• W4387447918 modified "2023-10-11" @default.
• W4387447918 title "Impact of Targeting Moiety Type and Protein Corona Formation on the Uptake of Fn14-Targeted Nanoparticles by Cancer Cells" @default.
• W4387447918 cites W1526128150 @default.
• W4387447918 cites W1537721792 @default.
• W4387447918 cites W1562444807 @default.
• W4387447918 cites W1656255119 @default.
• W4387447918 cites W1809800295 @default.
• W4387447918 cites W1965063429 @default.
• W4387447918 cites W1968134260 @default.
• W4387447918 cites W1968399222 @default.
• W4387447918 cites W1972461780 @default.
• W4387447918 cites W1993373375 @default.
• W4387447918 cites W1996219643 @default.
• W4387447918 cites W2002528172 @default.
• W4387447918 cites W2002729585 @default.
• W4387447918 cites W2002936944 @default.
• W4387447918 cites W2005335834 @default.
• W4387447918 cites W2011601805 @default.
• W4387447918 cites W2011984976 @default.
• W4387447918 cites W2012186010 @default.
• W4387447918 cites W2015006203 @default.
• W4387447918 cites W2015513114 @default.
• W4387447918 cites W2039980903 @default.
• W4387447918 cites W2053369403 @default.
• W4387447918 cites W2055579880 @default.
• W4387447918 cites W2060544357 @default.
• W4387447918 cites W2061476447 @default.
• W4387447918 cites W2063170675 @default.
• W4387447918 cites W2074049796 @default.
• W4387447918 cites W2078944426 @default.
• W4387447918 cites W2083221204 @default.
• W4387447918 cites W2087824631 @default.
• W4387447918 cites W2089974427 @default.
• W4387447918 cites W2090001834 @default.
• W4387447918 cites W2095854326 @default.
• W4387447918 cites W2100682112 @default.
• W4387447918 cites W2110634891 @default.
• W4387447918 cites W2116661011 @default.
• W4387447918 cites W2117084436 @default.
• W4387447918 cites W2124733101 @default.
• W4387447918 cites W2126462892 @default.
• W4387447918 cites W2131244161 @default.
• W4387447918 cites W2139333712 @default.
• W4387447918 cites W2157016784 @default.
• W4387447918 cites W2161946327 @default.
• W4387447918 cites W2165799545 @default.
• W4387447918 cites W2167279371 @default.
• W4387447918 cites W2172260616 @default.
• W4387447918 cites W2478243587 @default.
• W4387447918 cites W2520629744 @default.
• W4387447918 cites W2531611585 @default.
• W4387447918 cites W2554773181 @default.
• W4387447918 cites W2615015413 @default.
• W4387447918 cites W2724290859 @default.
• W4387447918 cites W2734985127 @default.
• W4387447918 cites W2748142694 @default.
• W4387447918 cites W2751184387 @default.
• W4387447918 cites W2755462565 @default.
• W4387447918 cites W2769353100 @default.
• W4387447918 cites W2773229707 @default.
• W4387447918 cites W2773580742 @default.
• W4387447918 cites W2793807497 @default.
• W4387447918 cites W2800587747 @default.
• W4387447918 cites W2800726265 @default.
• W4387447918 cites W2893010176 @default.
• W4387447918 cites W2898415565 @default.
• W4387447918 cites W2913512601 @default.
• W4387447918 cites W2942862519 @default.
• W4387447918 cites W2949844530 @default.
• W4387447918 cites W2950187185 @default.
• W4387447918 cites W2967740108 @default.
• W4387447918 cites W2968785382 @default.
• W4387447918 cites W2976254154 @default.
• W4387447918 cites W2995256698 @default.
• W4387447918 cites W3000096514 @default.
• W4387447918 cites W3001632525 @default.
• W4387447918 cites W3007476727 @default.
• W4387447918 cites W3040590863 @default.
• W4387447918 cites W3135875197 @default.
• W4387447918 cites W3137488465 @default.
• W4387447918 cites W3181771104 @default.
• W4387447918 cites W3208275680 @default.
• W4387447918 cites W4211220080 @default.
• W4387447918 cites W4221082366 @default.
• W4387447918 cites W4225683993 @default.
• W4387447918 cites W4289341900 @default.
• W4387447918 cites W4308959157 @default.
• W4387447918 cites W4313436441 @default.

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