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- W4387452561 abstract "Abstract Prokineticin 2 (Prok2) is a small protein expressed in a subpopulation of neurons in the suprachiasmatic nucleus (SCN), the primary circadian pacemaker in mammals. Prok2 has been implicated as a candidate output molecule from the SCN to control multiple circadian rhythms. Genetic manipulation specific to Prok2-producing neurons would be a powerful approach to understanding their function. Here, we report the generation of Prok2-tTA knock-in mice expressing the tetracycline transactivator (tTA) specifically in Prok2 neurons and an application of these mice to in vivo recording of Ca 2+ rhythms in these neurons. First, the specific and efficient expression of tTA in Prok2 neurons was verified by crossing the mice with EGFP reporter mice. Prok2-tTA mice were then used to express a fluorescent Ca 2+ sensor protein to record the circadian Ca 2+ rhythm in SCN Prok2 neurons in vivo. Ca 2+ in these cells showed clear circadian rhythms in both light–dark and constant dark conditions, with their peaks around midday. Notably, the hours of high Ca 2+ nearly coincided with the rest period of the behavioral rhythm. These observations fit well with the predicted function of Prok2 neurons as a candidate output pathway of the SCN by suppressing locomotor activity during both daytime and subjective daytime." @default.
- W4387452561 created "2023-10-10" @default.
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- W4387452561 date "2023-10-09" @default.
- W4387452561 modified "2023-10-12" @default.
- W4387452561 title "In vivo recording of the circadian calcium rhythm in Prokineticin 2 neurons of the suprachiasmatic nucleus" @default.
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- W4387452561 doi "https://doi.org/10.1038/s41598-023-44282-5" @default.
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