FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387460721> ?p ?o ?g. }

• W4387460721 abstract "Abstract Since the end of 2020, the SARS-CoV-2 strain has undergone several mutations, and new variants continue to emerge. The immune escape ability of the mutants has enhanced, showing robust transmissibility. The neutralizing ability of the neutralizing antibodies produced during previous infections decreased in some mutants. This poses a severe challenge to the preventive and therapeutic effectiveness of vaccines and antibody drugs. Nucleocapsid protein, one of the main structural proteins of the coronavirus, plays an important role in the life cycle of the novel coronavirus and has proven to be one of the key targets for drug development. The first key step in drug development is to obtain pure nucleocapsid proteins. However, as nucleocapsid proteins have a nucleic acid binding function and can automatically undergo liquid–liquid phase separation and agglomerate, the purification of full-length nucleocapsids is extremely challenging. In this study, a set of easy-to-operate processes for the purification of nucleocapsid protein was developed. Finally, a pure full-length nucleocapsid protein without nucleic acid contamination was obtained. Subsequently, we also targeted the nucleic acid binding domain of the nucleocapsid protein and screened for potential SARS-CoV-2 inhibitors using virtual screening and biolayer interferometry (BLI) technology. Finally, a small molecule inhibitor, Light Green SF ( K D = 19.9 µM), which can bind to nucleocapsid protein, was identified. In the future, we will continue to conduct more in-depth research and attempt to develop drugs that possess a good inhibitory effect on the current novel coronavirus mutants." @default.
• W4387460721 created "2023-10-10" @default.
• W4387460721 creator A5023257717 @default.
• W4387460721 creator A5042522014 @default.
• W4387460721 creator A5057350721 @default.
• W4387460721 creator A5063253432 @default.
• W4387460721 creator A5071324447 @default.
• W4387460721 date "2023-10-09" @default.
• W4387460721 modified "2023-10-11" @default.
• W4387460721 title "Purification and inhibitor screening of the full-length SARS-CoV-2 nucleocapsid protein" @default.
• W4387460721 cites W1591681043 @default.
• W4387460721 cites W2008552982 @default.
• W4387460721 cites W2010974713 @default.
• W4387460721 cites W2069104829 @default.
• W4387460721 cites W3085912969 @default.
• W4387460721 cites W3096967708 @default.
• W4387460721 cites W3113408297 @default.
• W4387460721 cites W3125800928 @default.
• W4387460721 cites W3126729284 @default.
• W4387460721 cites W3139080744 @default.
• W4387460721 cites W3144228652 @default.
• W4387460721 cites W3149429522 @default.
• W4387460721 cites W3164603734 @default.
• W4387460721 cites W3165403061 @default.
• W4387460721 cites W3165479483 @default.
• W4387460721 cites W3168384292 @default.
• W4387460721 cites W3179152893 @default.
• W4387460721 cites W3190159554 @default.
• W4387460721 cites W3197529927 @default.
• W4387460721 cites W3201939839 @default.
• W4387460721 cites W3207605229 @default.
• W4387460721 cites W3209795798 @default.
• W4387460721 cites W4200429845 @default.
• W4387460721 cites W4200590114 @default.
• W4387460721 cites W4206424900 @default.
• W4387460721 cites W4207041895 @default.
• W4387460721 cites W4210332283 @default.
• W4387460721 cites W4220788616 @default.
• W4387460721 cites W4220899576 @default.
• W4387460721 cites W4220914888 @default.
• W4387460721 cites W4221057725 @default.
• W4387460721 cites W4285820039 @default.
• W4387460721 cites W4313985041 @default.
• W4387460721 doi "https://doi.org/10.21203/rs.3.rs-3420562/v1" @default.
• W4387460721 hasPublicationYear "2023" @default.
• W4387460721 type Work @default.
• W4387460721 citedByCount "0" @default.
• W4387460721 crossrefType "posted-content" @default.
• W4387460721 hasAuthorship W4387460721A5023257717 @default.
• W4387460721 hasAuthorship W4387460721A5042522014 @default.
• W4387460721 hasAuthorship W4387460721A5057350721 @default.
• W4387460721 hasAuthorship W4387460721A5063253432 @default.
• W4387460721 hasAuthorship W4387460721A5071324447 @default.
• W4387460721 hasBestOaLocation W43874607211 @default.
• W4387460721 hasConcept C104317684 @default.
• W4387460721 hasConcept C142724271 @default.
• W4387460721 hasConcept C143065580 @default.
• W4387460721 hasConcept C159047783 @default.
• W4387460721 hasConcept C159654299 @default.
• W4387460721 hasConcept C185592680 @default.
• W4387460721 hasConcept C24107716 @default.
• W4387460721 hasConcept C2777648638 @default.
• W4387460721 hasConcept C2779134260 @default.
• W4387460721 hasConcept C3007834351 @default.
• W4387460721 hasConcept C3008058167 @default.
• W4387460721 hasConcept C524204448 @default.
• W4387460721 hasConcept C54355233 @default.
• W4387460721 hasConcept C55493867 @default.
• W4387460721 hasConcept C70721500 @default.
• W4387460721 hasConcept C71924100 @default.
• W4387460721 hasConcept C74187038 @default.
• W4387460721 hasConcept C86803240 @default.
• W4387460721 hasConceptScore W4387460721C104317684 @default.
• W4387460721 hasConceptScore W4387460721C142724271 @default.
• W4387460721 hasConceptScore W4387460721C143065580 @default.
• W4387460721 hasConceptScore W4387460721C159047783 @default.
• W4387460721 hasConceptScore W4387460721C159654299 @default.
• W4387460721 hasConceptScore W4387460721C185592680 @default.
• W4387460721 hasConceptScore W4387460721C24107716 @default.
• W4387460721 hasConceptScore W4387460721C2777648638 @default.
• W4387460721 hasConceptScore W4387460721C2779134260 @default.
• W4387460721 hasConceptScore W4387460721C3007834351 @default.
• W4387460721 hasConceptScore W4387460721C3008058167 @default.
• W4387460721 hasConceptScore W4387460721C524204448 @default.
• W4387460721 hasConceptScore W4387460721C54355233 @default.
• W4387460721 hasConceptScore W4387460721C55493867 @default.
• W4387460721 hasConceptScore W4387460721C70721500 @default.
• W4387460721 hasConceptScore W4387460721C71924100 @default.
• W4387460721 hasConceptScore W4387460721C74187038 @default.
• W4387460721 hasConceptScore W4387460721C86803240 @default.
• W4387460721 hasLocation W43874607211 @default.
• W4387460721 hasOpenAccess W4387460721 @default.
• W4387460721 hasPrimaryLocation W43874607211 @default.
• W4387460721 hasRelatedWork W1543504406 @default.
• W4387460721 hasRelatedWork W1971444612 @default.
• W4387460721 hasRelatedWork W1991445197 @default.
• W4387460721 hasRelatedWork W2155835698 @default.
• W4387460721 hasRelatedWork W2383750441 @default.
• W4387460721 hasRelatedWork W3002812395 @default.
• W4387460721 hasRelatedWork W3023213585 @default.

• next