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• W4387472605 abstract "Fluorescent D-amino acids (FDAAs) enable in situ visualization of bacterial cell wall synthesis via their incorporation into peptidoglycan (PG) crosslinks. When combined with super-resolution microscopy, FDAAs allow the details of cell wall synthesis to be resolved beyond the diffraction limit of visible light. Here, we describe using the super-resolution method of single-molecule localization microscopy (SMLM) in conjunction with two newly synthesized FDAAs (sCy5DA and sCy5DL_amide) to resolve bacterial PG at the nanoscale in a variety of species, including Gram-negative, Gram-positive, and mycobacteria." @default.
• W4387472605 created "2023-10-11" @default.
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• W4387472605 date "2023-10-11" @default.
• W4387472605 modified "2023-10-12" @default.
• W4387472605 title "Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids" @default.
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• W4387472605 doi "https://doi.org/10.1007/978-1-0716-3491-2_7" @default.
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