Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387478093> ?p ?o ?g. }
- W4387478093 endingPage "168312" @default.
- W4387478093 startingPage "168312" @default.
- W4387478093 abstract "Photoactivated adenylate cyclases (PACs) are light-activated enzymes that combine a BLUF (blue-light using flavin) domain and an adenylate cyclase domain that are able to increase the levels of the important second messenger cAMP (cyclic adenosine monophosphate) upon blue-light excitation. The light-induced changes in the BLUF domain are transduced to the adenylate cyclase domain via a mechanism that has not yet been established. One critical residue in the photoactivation mechanism of BLUF domains, present in the vicinity of the flavin is the glutamine amino acid close to the N5 of the flavin. The role of this residue has been investigated extensively both experimentally and theoretically. However, its role in the activity of the photoactivated adenylate cyclase, OaPAC has never been addressed. In this work, we applied ultrafast transient visible and infrared spectroscopies to study the photochemistry of the Q48E OaPAC mutant. This mutation altered the primary electron transfer process and switched the enzyme into a permanent ‘on’ state, able to increase the cAMP levels under dark conditions compared to the cAMP levels of the dark-adapted state of the wild-type OaPAC. Differential scanning calorimetry measurements point to a less compact structure for the Q48E OaPAC mutant. The ensemble of these findings provide insight into the important elements in PACs and how their fine tuning may help in the design of optogenetic devices." @default.
- W4387478093 created "2023-10-11" @default.
- W4387478093 creator A5010179746 @default.
- W4387478093 creator A5010883447 @default.
- W4387478093 creator A5013710650 @default.
- W4387478093 creator A5013967849 @default.
- W4387478093 creator A5018378717 @default.
- W4387478093 creator A5056035303 @default.
- W4387478093 creator A5064417354 @default.
- W4387478093 creator A5064452604 @default.
- W4387478093 creator A5078205705 @default.
- W4387478093 creator A5079884586 @default.
- W4387478093 creator A5084486939 @default.
- W4387478093 creator A5090045840 @default.
- W4387478093 creator A5093033217 @default.
- W4387478093 date "2023-10-01" @default.
- W4387478093 modified "2023-10-18" @default.
- W4387478093 title "Single amino acid mutation decouples photochemistry of the BLUF domain from the enzymatic function of OaPAC and drives the enzyme to a switched-on state" @default.
- W4387478093 cites W1571817040 @default.
- W4387478093 cites W1970289793 @default.
- W4387478093 cites W1973582217 @default.
- W4387478093 cites W1977547866 @default.
- W4387478093 cites W1987419575 @default.
- W4387478093 cites W1988129969 @default.
- W4387478093 cites W1993964147 @default.
- W4387478093 cites W1995295460 @default.
- W4387478093 cites W1995508450 @default.
- W4387478093 cites W2001969299 @default.
- W4387478093 cites W2004670135 @default.
- W4387478093 cites W2008126427 @default.
- W4387478093 cites W2010056946 @default.
- W4387478093 cites W2011668639 @default.
- W4387478093 cites W2012029494 @default.
- W4387478093 cites W2013417793 @default.
- W4387478093 cites W2016812646 @default.
- W4387478093 cites W2023347568 @default.
- W4387478093 cites W2023433221 @default.
- W4387478093 cites W2027922366 @default.
- W4387478093 cites W2031968879 @default.
- W4387478093 cites W2034328848 @default.
- W4387478093 cites W2040362515 @default.
- W4387478093 cites W2043673715 @default.
- W4387478093 cites W2044206194 @default.
- W4387478093 cites W2045013780 @default.
- W4387478093 cites W2058106995 @default.
- W4387478093 cites W2068590649 @default.
- W4387478093 cites W2070710546 @default.
- W4387478093 cites W2078184681 @default.
- W4387478093 cites W2080802206 @default.
- W4387478093 cites W2080862141 @default.
- W4387478093 cites W2081143109 @default.
- W4387478093 cites W2082545300 @default.
- W4387478093 cites W2085329207 @default.
- W4387478093 cites W2092830401 @default.
- W4387478093 cites W2111177172 @default.
- W4387478093 cites W2125185550 @default.
- W4387478093 cites W2133879022 @default.
- W4387478093 cites W2137347727 @default.
- W4387478093 cites W2156855465 @default.
- W4387478093 cites W2162874893 @default.
- W4387478093 cites W2166628073 @default.
- W4387478093 cites W2167711477 @default.
- W4387478093 cites W2290989114 @default.
- W4387478093 cites W2401809141 @default.
- W4387478093 cites W2415890418 @default.
- W4387478093 cites W2550298280 @default.
- W4387478093 cites W2603597834 @default.
- W4387478093 cites W2751611403 @default.
- W4387478093 cites W2767008740 @default.
- W4387478093 cites W2773284927 @default.
- W4387478093 cites W2897925329 @default.
- W4387478093 cites W2904460608 @default.
- W4387478093 cites W2945131650 @default.
- W4387478093 cites W2991190161 @default.
- W4387478093 cites W3005314615 @default.
- W4387478093 cites W3080760933 @default.
- W4387478093 cites W3118281429 @default.
- W4387478093 cites W3133331789 @default.
- W4387478093 cites W3179241694 @default.
- W4387478093 cites W4206526530 @default.
- W4387478093 cites W4225115637 @default.
- W4387478093 cites W4283324786 @default.
- W4387478093 cites W4293681077 @default.
- W4387478093 cites W4294990658 @default.
- W4387478093 cites W4313595589 @default.
- W4387478093 cites W4318754951 @default.
- W4387478093 doi "https://doi.org/10.1016/j.jmb.2023.168312" @default.
- W4387478093 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37827329" @default.
- W4387478093 hasPublicationYear "2023" @default.
- W4387478093 type Work @default.
- W4387478093 citedByCount "0" @default.
- W4387478093 crossrefType "journal-article" @default.
- W4387478093 hasAuthorship W4387478093A5010179746 @default.
- W4387478093 hasAuthorship W4387478093A5010883447 @default.
- W4387478093 hasAuthorship W4387478093A5013710650 @default.
- W4387478093 hasAuthorship W4387478093A5013967849 @default.
- W4387478093 hasAuthorship W4387478093A5018378717 @default.
- W4387478093 hasAuthorship W4387478093A5056035303 @default.