FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387490518> ?p ?o ?g. }

• W4387490518 abstract "In this study, a suite of efficient CRISPR/Cas9 tools was developed to overcome the genetic manipulation challenges posed by the polyploid genome of industrial yeast Cyberlindnera jadinii. The developed CRISPR/Cas9 system can achieve a 100% single-gene knockdown efficiency in strain NBRC0988. Moreover, the integration of a single exogenous gene into the target locus using a 50 bp homology arm achieved near-100% efficiency. The efficiency of simultaneous integration of three genes into the chromosome is strongly influenced by the length of the homology arm, with the highest integration efficiency of 62.5% obtained when selecting a homology arm of about 500 bp. By utilizing the CRISPR/Cas system, this study demonstrated the potential of C. jadinii in producing heterologous sterols. Through shake-flask fermentation, the engineered strains produced 92.1 and 81.8 mg/L of campesterol and cholesterol, respectively. Furthermore, the production levels of these two sterols were further enhanced through high-cell-density fed-batch fermentation in a 5 L bioreactor. The highest titer of campesterol reached 807 mg/L [biomass OD600 = 294, productivity of 6.73 mg/(L·h)]. The titer of cholesterol reached 1.52 g/L [biomass OD600 = 380, productivity of 9.06 mg/(L·h)], marking the first gram-scale production of steroidal compounds in C. jadinii." @default.
• W4387490518 created "2023-10-11" @default.
• W4387490518 creator A5003866126 @default.
• W4387490518 creator A5007708850 @default.
• W4387490518 creator A5017492904 @default.
• W4387490518 creator A5024124240 @default.
• W4387490518 creator A5026647970 @default.
• W4387490518 creator A5050791399 @default.
• W4387490518 creator A5063189579 @default.
• W4387490518 creator A5072585501 @default.
• W4387490518 creator A5074359987 @default.
• W4387490518 creator A5091091778 @default.
• W4387490518 date "2023-10-10" @default.
• W4387490518 modified "2023-10-12" @default.
• W4387490518 title "Development of CRISPR/Cas9-Based Genome Editing Tools for Polyploid Yeast <i>Cyberlindnera jadinii</i> and Its Application in Engineering Heterologous Steroid-Producing Strains" @default.
• W4387490518 cites W119702789 @default.
• W4387490518 cites W1888035769 @default.
• W4387490518 cites W1969218431 @default.
• W4387490518 cites W1987196385 @default.
• W4387490518 cites W1990009960 @default.
• W4387490518 cites W1992783297 @default.
• W4387490518 cites W1996595039 @default.
• W4387490518 cites W2011248736 @default.
• W4387490518 cites W2017550964 @default.
• W4387490518 cites W2029995786 @default.
• W4387490518 cites W2034776719 @default.
• W4387490518 cites W2039705224 @default.
• W4387490518 cites W2042649558 @default.
• W4387490518 cites W2048108455 @default.
• W4387490518 cites W2052771420 @default.
• W4387490518 cites W2054124438 @default.
• W4387490518 cites W2056928998 @default.
• W4387490518 cites W2061961947 @default.
• W4387490518 cites W2072369409 @default.
• W4387490518 cites W2079850481 @default.
• W4387490518 cites W2083281099 @default.
• W4387490518 cites W2087118474 @default.
• W4387490518 cites W2094581370 @default.
• W4387490518 cites W2104577816 @default.
• W4387490518 cites W2115420193 @default.
• W4387490518 cites W2133836136 @default.
• W4387490518 cites W2148669374 @default.
• W4387490518 cites W2149429041 @default.
• W4387490518 cites W2154018668 @default.
• W4387490518 cites W2154431984 @default.
• W4387490518 cites W2165034669 @default.
• W4387490518 cites W2166116807 @default.
• W4387490518 cites W2217013086 @default.
• W4387490518 cites W2237139796 @default.
• W4387490518 cites W2316631252 @default.
• W4387490518 cites W2521969424 @default.
• W4387490518 cites W2555624605 @default.
• W4387490518 cites W2561030438 @default.
• W4387490518 cites W262452561 @default.
• W4387490518 cites W2654116011 @default.
• W4387490518 cites W2783508188 @default.
• W4387490518 cites W2811323887 @default.
• W4387490518 cites W2888407023 @default.
• W4387490518 cites W2929740617 @default.
• W4387490518 cites W2951461412 @default.
• W4387490518 cites W2954420967 @default.
• W4387490518 cites W2964849163 @default.
• W4387490518 cites W3091553461 @default.
• W4387490518 cites W3118430678 @default.
• W4387490518 cites W3129934448 @default.
• W4387490518 cites W3164582289 @default.
• W4387490518 cites W4206960949 @default.
• W4387490518 cites W4210526643 @default.
• W4387490518 cites W4297181825 @default.
• W4387490518 doi "https://doi.org/10.1021/acssynbio.3c00278" @default.
• W4387490518 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37816156" @default.
• W4387490518 hasPublicationYear "2023" @default.
• W4387490518 type Work @default.
• W4387490518 citedByCount "0" @default.
• W4387490518 crossrefType "journal-article" @default.
• W4387490518 hasAuthorship W4387490518A5003866126 @default.
• W4387490518 hasAuthorship W4387490518A5007708850 @default.
• W4387490518 hasAuthorship W4387490518A5017492904 @default.
• W4387490518 hasAuthorship W4387490518A5024124240 @default.
• W4387490518 hasAuthorship W4387490518A5026647970 @default.
• W4387490518 hasAuthorship W4387490518A5050791399 @default.
• W4387490518 hasAuthorship W4387490518A5063189579 @default.
• W4387490518 hasAuthorship W4387490518A5072585501 @default.
• W4387490518 hasAuthorship W4387490518A5074359987 @default.
• W4387490518 hasAuthorship W4387490518A5091091778 @default.
• W4387490518 hasConcept C104317684 @default.
• W4387490518 hasConcept C132455925 @default.
• W4387490518 hasConcept C141231307 @default.
• W4387490518 hasConcept C144501496 @default.
• W4387490518 hasConcept C150903083 @default.
• W4387490518 hasConcept C2776626444 @default.
• W4387490518 hasConcept C2780115692 @default.
• W4387490518 hasConcept C54355233 @default.
• W4387490518 hasConcept C6350086 @default.
• W4387490518 hasConcept C70721500 @default.
• W4387490518 hasConcept C86803240 @default.
• W4387490518 hasConcept C98108389 @default.
• W4387490518 hasConceptScore W4387490518C104317684 @default.
• W4387490518 hasConceptScore W4387490518C132455925 @default.
• W4387490518 hasConceptScore W4387490518C141231307 @default.

• next