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- W4387491279 abstract "Rubus alceifolius Poir (R.A. Poir) leaves are rich in phenolic compounds, offering many health benefits due to their incredible antioxidant potential. In this study, conditions for the ultrasound-assisted extraction (UAE) of phenolic compounds and antioxidant activity from R.A. Poir leaves were optimized using response surface methodology (RSM). This methodology assessed the effects of ultrasound power ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M1> <msub> <mrow> <mi>X</mi> </mrow> <mrow> <mn>1</mn> </mrow> </msub> </math> : 100-500 W), extraction temperature ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M2> <msub> <mrow> <mi>X</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msub> </math> : 30-60°C), and extraction time ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M3> <msub> <mrow> <mi>X</mi> </mrow> <mrow> <mn>3</mn> </mrow> </msub> </math> : 5-55 min). The optimized UAE conditions were then compared with conventional extraction methods (Soxhlet extraction: SE and maceration extraction: ME) for extracting total phenolics. A phenolic profile using GC-MS and antioxidant activity (ABTS) was also compared. According to the RSM, the best conditions for UAE to extract the highest total polyphenol content and ABTS radical scavenging activity were 320 W ultrasound power, 40°C extraction temperature, and 35.5 min sonication duration. Under these optimal conditions, the TPC and antioxidant activity reached 16.68 mg GAE/g dm and 21.9 mg TE/g, respectively, closely aligning with the predicted values. The UAE extraction technique proved to be more efficient in extracting phenolics and antioxidant capacity (ABTS (2,2 <math xmlns=http://www.w3.org/1998/Math/MathML id=M4> <mo>′</mo> </math> -azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)) radical scavenging activity, and enzyme inhibition) compared to the conventional extraction methods (SE and ME). A GC-MS analysis identified 12 components, including 5 phenolics and 3 flavonoids, which likely contribute to the antioxidant activity. Consequently, the UAE method improved extraction efficiency within a shorter time frame, suggesting that UAE is a promising, efficient, and ecofriendly technology for extracting bioactive compounds from R.A. Poir leaves." @default.
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- W4387491279 date "2023-10-10" @default.
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- W4387491279 title "Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves" @default.
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- W4387491279 doi "https://doi.org/10.1155/2023/7576179" @default.
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