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- W4387491457 abstract "Cross-linking mass spectrometry (MS) is currently transitioning from a routine tool in structural biology to enabling structural systems biology. MS-cleavable cross-linkers could substantially reduce the associated search space expansion by allowing a MS3-based approach for identifying cross-linked peptides. However, MS2 (MS/MS)-based approaches currently outperform approaches utilizing MS3. We show here that the sensitivity and specificity of triggering MS3 have been hampered algorithmically. Our four-step MS3-trigger algorithm greatly outperformed currently employed methods and comes close to reaching the theoretical limit." @default.
- W4387491457 created "2023-10-11" @default.
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- W4387491457 date "2023-10-10" @default.
- W4387491457 modified "2023-10-16" @default.
- W4387491457 title "Cleavable Cross-Linkers Redefined by a Novel MS<sup>3</sup>-Trigger Algorithm" @default.
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- W4387491457 doi "https://doi.org/10.1021/acs.analchem.3c01673" @default.
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