Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387497736> ?p ?o ?g. }
- W4387497736 endingPage "4916" @default.
- W4387497736 startingPage "4916" @default.
- W4387497736 abstract "Radiation therapy (RT) is a crucial treatment modality for central nervous system (CNS) tumors but toxicity to healthy CNS tissues remains a challenge. Additionally, environmental exposure to radiation during nuclear catastrophes or space travel presents a risk of CNS toxicity. However, the underlying mechanisms of radiation-induced CNS toxicity are not fully understood. Neural progenitor cells (NPCs) are highly radiosensitive, resulting in decreased neurogenesis in the hippocampus. This study aimed to characterize a novel platform utilizing rat NPCs cultured as 3D neurospheres (NSps) to screen the safety and efficacy of experimental drugs with and without radiation exposure. The effect of radiation on NSp growth and differentiation was assessed by measuring sphere volume and the expression of neuronal differentiation markers Nestin and GFAP and proliferation marker Ki67. Radiation exposure inhibited NSp growth, decreased proliferation, and increased GFAP expression, indicating astrocytic differentiation. RNA sequencing analysis supported these findings, showing upregulation of Notch, BMP2/4, S100b, and GFAP gene expression during astrogenesis. By recapitulating radiation-induced toxicity and astrocytic differentiation, this single-NSp culture system provides a high-throughput preclinical model for assessing the effects of various radiation modalities and evaluates the safety and efficacy of potential therapeutic interventions in combination with radiation." @default.
- W4387497736 created "2023-10-11" @default.
- W4387497736 creator A5003700517 @default.
- W4387497736 creator A5031514517 @default.
- W4387497736 creator A5044413521 @default.
- W4387497736 creator A5050418297 @default.
- W4387497736 creator A5056576680 @default.
- W4387497736 creator A5058320928 @default.
- W4387497736 creator A5062263859 @default.
- W4387497736 creator A5066552417 @default.
- W4387497736 date "2023-10-10" @default.
- W4387497736 modified "2023-10-12" @default.
- W4387497736 title "Development of a Single-Neurosphere Culture to Assess Radiation Toxicity and Pre-Clinical Cancer Combination Therapy Safety" @default.
- W4387497736 cites W1485371658 @default.
- W4387497736 cites W1849188294 @default.
- W4387497736 cites W1891165767 @default.
- W4387497736 cites W1901389472 @default.
- W4387497736 cites W1964223452 @default.
- W4387497736 cites W1975276783 @default.
- W4387497736 cites W1983539927 @default.
- W4387497736 cites W2006180302 @default.
- W4387497736 cites W2009129665 @default.
- W4387497736 cites W2019264546 @default.
- W4387497736 cites W2023011577 @default.
- W4387497736 cites W2025201512 @default.
- W4387497736 cites W2031324184 @default.
- W4387497736 cites W2035833991 @default.
- W4387497736 cites W2038368018 @default.
- W4387497736 cites W2045253310 @default.
- W4387497736 cites W2079356804 @default.
- W4387497736 cites W2093825890 @default.
- W4387497736 cites W2094660330 @default.
- W4387497736 cites W2098137179 @default.
- W4387497736 cites W2113249378 @default.
- W4387497736 cites W2115438940 @default.
- W4387497736 cites W2136671298 @default.
- W4387497736 cites W2141351251 @default.
- W4387497736 cites W2404218280 @default.
- W4387497736 cites W2577266312 @default.
- W4387497736 cites W2626725994 @default.
- W4387497736 cites W2793269909 @default.
- W4387497736 cites W2946753897 @default.
- W4387497736 cites W2955578490 @default.
- W4387497736 cites W2990405159 @default.
- W4387497736 cites W3021207951 @default.
- W4387497736 cites W3092615318 @default.
- W4387497736 cites W3120901283 @default.
- W4387497736 cites W4200559809 @default.
- W4387497736 cites W4220981780 @default.
- W4387497736 cites W4283026937 @default.
- W4387497736 cites W4288736974 @default.
- W4387497736 cites W4296164166 @default.
- W4387497736 cites W4303628714 @default.
- W4387497736 cites W4308834893 @default.
- W4387497736 cites W4376116923 @default.
- W4387497736 doi "https://doi.org/10.3390/cancers15204916" @default.
- W4387497736 hasPublicationYear "2023" @default.
- W4387497736 type Work @default.
- W4387497736 citedByCount "0" @default.
- W4387497736 crossrefType "journal-article" @default.
- W4387497736 hasAuthorship W4387497736A5003700517 @default.
- W4387497736 hasAuthorship W4387497736A5031514517 @default.
- W4387497736 hasAuthorship W4387497736A5044413521 @default.
- W4387497736 hasAuthorship W4387497736A5050418297 @default.
- W4387497736 hasAuthorship W4387497736A5056576680 @default.
- W4387497736 hasAuthorship W4387497736A5058320928 @default.
- W4387497736 hasAuthorship W4387497736A5062263859 @default.
- W4387497736 hasAuthorship W4387497736A5066552417 @default.
- W4387497736 hasBestOaLocation W43874977361 @default.
- W4387497736 hasConcept C104317684 @default.
- W4387497736 hasConcept C126322002 @default.
- W4387497736 hasConcept C136834591 @default.
- W4387497736 hasConcept C142724271 @default.
- W4387497736 hasConcept C148738053 @default.
- W4387497736 hasConcept C162746124 @default.
- W4387497736 hasConcept C169760540 @default.
- W4387497736 hasConcept C20875687 @default.
- W4387497736 hasConcept C2777738006 @default.
- W4387497736 hasConcept C28328180 @default.
- W4387497736 hasConcept C29730261 @default.
- W4387497736 hasConcept C4746552 @default.
- W4387497736 hasConcept C502942594 @default.
- W4387497736 hasConcept C509974204 @default.
- W4387497736 hasConcept C54355233 @default.
- W4387497736 hasConcept C71924100 @default.
- W4387497736 hasConcept C86803240 @default.
- W4387497736 hasConcept C95444343 @default.
- W4387497736 hasConceptScore W4387497736C104317684 @default.
- W4387497736 hasConceptScore W4387497736C126322002 @default.
- W4387497736 hasConceptScore W4387497736C136834591 @default.
- W4387497736 hasConceptScore W4387497736C142724271 @default.
- W4387497736 hasConceptScore W4387497736C148738053 @default.
- W4387497736 hasConceptScore W4387497736C162746124 @default.
- W4387497736 hasConceptScore W4387497736C169760540 @default.
- W4387497736 hasConceptScore W4387497736C20875687 @default.
- W4387497736 hasConceptScore W4387497736C2777738006 @default.
- W4387497736 hasConceptScore W4387497736C28328180 @default.
- W4387497736 hasConceptScore W4387497736C29730261 @default.