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• W4387523272 abstract "The aim was to evaluate whether the doses of orange essential oil supplementation impact on dry matter intake (DMI), ruminal fermentation parameters, apparent nutrient digestibility, and methane production in Nellore steers fed with forage-based diet. A total of 32 rumen-cannulated Nellore steers (initial BW= 335±25 kg; age= 20±1 month) were assigned to individual pens in a randomized complete block design. Within block, steers were randomly assigned to treatments: CON= diet without orange essential oil (EO); 100EO= CON diet plus 100 mg orange EO/kg DM diet; 200EO= CON diet plus 200 mg orange EO/kg DM diet; and 500EO= CON diet plus 500 mg orange EO/kg DM diet. The main active compounds of the orange EO used were limonene (88.0%) and myrcene (5.79%). The experimental period lasted 28d, with 22d for adaptation and 6d for sample collection. From d23 to 27, samples of haylage, concentrate, and total fecal production were collected to determine the total tract apparent nutrient digestibility. Ruminal fluid was obtained from d28 at 0, 6, and 12h after feeding to determine ruminal fermentation parameters and methane production. The difference was considered significant when P≤0.05. No treatment effects were detected (P>0.05) to DMI or specific nutrient intake. The increasing levels of EO did not affect (P>0.05) the total apparent nutrient digestibility. No treatment effects were detected (P>0.05) for ruminal fermentation parameters in vivo. In ex situ, there was a treatment effect for acetate, total short-chain fatty acids (SCFA), methane, and relative energy loss (REL). For acetate, an increasing linear effect was observed for treatments that received EO for the variables production (g/kg) (P=0.002) and gross energy (MJ/kg) (P=0.002), with a higher concentration for the 500EO. For the total production of SCFA, there was an increasing linear effect of treatment with a higher concentration for 500EO for the variables production (g/kg) (P=0.001) and gross energy (MJ/kg) (P=0.001). For methane, a quadratic treatment effect was observed for the variables production (g/kg) (P=0.045) and gross energy (MJ/kg) (P=0.046), with lower concentrations for the 200EO. There was a decreasing linear treatment effect for REL(%) (P<0.001). The results for the use of orange EO as a manipulator of ruminal fermentation were promising and the dose of 500 mg/kg of DM showed a greater capacity to increase the energy efficiency of fermentation, with greater production of acids and lower production of methane, without affecting the voluntary intake of steers." @default.
• W4387523272 created "2023-10-12" @default.
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• W4387523272 date "2023-10-01" @default.
• W4387523272 modified "2023-10-12" @default.
• W4387523272 title "Doses of orange (Citrus sinensis) essential oil for Nellore steers fed with a forage-based diet" @default.
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• W4387523272 doi "https://doi.org/10.1016/j.livsci.2023.105357" @default.
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