FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387526099> ?p ?o ?g. }

• W4387526099 endingPage "242" @default.
• W4387526099 startingPage "232" @default.
• W4387526099 abstract "L-aspartate is an important chemical in the food and pharmaceutical industries. Presently, it is meaningful to realize a co-immobilized multi-enzyme biocatalyst that can synthesize L-aspartate in vitro through multi-step cascade reaction. Herein, the immobilization method was first employed to investigate the ability of immobilized double enzymes, isomerase AaMaiA and aspartase AspB, to systhesize L-aspartate. The optimal temperature (50 ℃) of AaMaiA-immobilized NH2-UiO66, a metal organic framework material, was significantly superior to that of its free counterpart. Compared to free enzymes, AaMaiA@NH2-UiO66 retained 57.8% of enzyme activity and exhibited a half-life that was improved 2.59-fold at 50 ℃. The co-immobilized AaMaiA/AspB@NH2-UiO66 (AaMaiA: AspB = 6: 1, v/v) showed high protein loading (27.899 ± 1.945 mg/g) and 84.4% recovery of enzyme activity after 8 consecutive cycles. Compared to free AaMaiA/AspB, the novel AaMaiA/AspB@NH2-UiO66 exhibited far superior tolerance to organic solvents. For the first time, the prepared AaMaiA/AspB@NH2-UiO66 was successfully applied as an efficient biocatalyst and converted 1 M maleate to L-aspartate with a yield of 86.8% within 1 h. Remarkably, AaMaiA/AspB@NH2-UiO66 exhibited great potential for efficient biosynthesis of L-aspartate." @default.
• W4387526099 created "2023-10-12" @default.
• W4387526099 creator A5006204934 @default.
• W4387526099 creator A5031843635 @default.
• W4387526099 creator A5041077007 @default.
• W4387526099 creator A5049341927 @default.
• W4387526099 creator A5067466764 @default.
• W4387526099 creator A5077557415 @default.
• W4387526099 date "2023-11-01" @default.
• W4387526099 modified "2023-10-15" @default.
• W4387526099 title "Novel co-immobilization of thermostable isomerase and aspartase on NH2-UiO66 for efficient biosynthesis of L-aspartate from maleate" @default.
• W4387526099 cites W1587690497 @default.
• W4387526099 cites W1898110215 @default.
• W4387526099 cites W1967807598 @default.
• W4387526099 cites W1977877548 @default.
• W4387526099 cites W2020528917 @default.
• W4387526099 cites W2036414407 @default.
• W4387526099 cites W2048443971 @default.
• W4387526099 cites W2068140367 @default.
• W4387526099 cites W2146194886 @default.
• W4387526099 cites W2188309357 @default.
• W4387526099 cites W2266239956 @default.
• W4387526099 cites W2407757741 @default.
• W4387526099 cites W2419280684 @default.
• W4387526099 cites W2609455338 @default.
• W4387526099 cites W2789593316 @default.
• W4387526099 cites W2889931462 @default.
• W4387526099 cites W2913045810 @default.
• W4387526099 cites W2937623982 @default.
• W4387526099 cites W2944023328 @default.
• W4387526099 cites W2949484779 @default.
• W4387526099 cites W2961825727 @default.
• W4387526099 cites W2971603564 @default.
• W4387526099 cites W2990139425 @default.
• W4387526099 cites W2991758999 @default.
• W4387526099 cites W3002946538 @default.
• W4387526099 cites W3013610085 @default.
• W4387526099 cites W3014475993 @default.
• W4387526099 cites W3023365446 @default.
• W4387526099 cites W3040779369 @default.
• W4387526099 cites W3138377336 @default.
• W4387526099 cites W3165073222 @default.
• W4387526099 cites W3177767616 @default.
• W4387526099 cites W3186833100 @default.
• W4387526099 cites W3195410443 @default.
• W4387526099 cites W4206117801 @default.
• W4387526099 cites W4211029584 @default.
• W4387526099 cites W4223456665 @default.
• W4387526099 cites W4229051380 @default.
• W4387526099 cites W4307494675 @default.
• W4387526099 doi "https://doi.org/10.1016/j.procbio.2023.10.012" @default.
• W4387526099 hasPublicationYear "2023" @default.
• W4387526099 type Work @default.
• W4387526099 citedByCount "0" @default.
• W4387526099 crossrefType "journal-article" @default.
• W4387526099 hasAuthorship W4387526099A5006204934 @default.
• W4387526099 hasAuthorship W4387526099A5031843635 @default.
• W4387526099 hasAuthorship W4387526099A5041077007 @default.
• W4387526099 hasAuthorship W4387526099A5049341927 @default.
• W4387526099 hasAuthorship W4387526099A5067466764 @default.
• W4387526099 hasAuthorship W4387526099A5077557415 @default.
• W4387526099 hasConcept C134121241 @default.
• W4387526099 hasConcept C13965031 @default.
• W4387526099 hasConcept C161790260 @default.
• W4387526099 hasConcept C181199279 @default.
• W4387526099 hasConcept C185592680 @default.
• W4387526099 hasConcept C191503008 @default.
• W4387526099 hasConcept C191897082 @default.
• W4387526099 hasConcept C192562407 @default.
• W4387526099 hasConcept C202751555 @default.
• W4387526099 hasConcept C27881333 @default.
• W4387526099 hasConcept C33633552 @default.
• W4387526099 hasConcept C553450214 @default.
• W4387526099 hasConcept C55493867 @default.
• W4387526099 hasConcept C65024703 @default.
• W4387526099 hasConcept C71240020 @default.
• W4387526099 hasConceptScore W4387526099C134121241 @default.
• W4387526099 hasConceptScore W4387526099C13965031 @default.
• W4387526099 hasConceptScore W4387526099C161790260 @default.
• W4387526099 hasConceptScore W4387526099C181199279 @default.
• W4387526099 hasConceptScore W4387526099C185592680 @default.
• W4387526099 hasConceptScore W4387526099C191503008 @default.
• W4387526099 hasConceptScore W4387526099C191897082 @default.
• W4387526099 hasConceptScore W4387526099C192562407 @default.
• W4387526099 hasConceptScore W4387526099C202751555 @default.
• W4387526099 hasConceptScore W4387526099C27881333 @default.
• W4387526099 hasConceptScore W4387526099C33633552 @default.
• W4387526099 hasConceptScore W4387526099C553450214 @default.
• W4387526099 hasConceptScore W4387526099C55493867 @default.
• W4387526099 hasConceptScore W4387526099C65024703 @default.
• W4387526099 hasConceptScore W4387526099C71240020 @default.
• W4387526099 hasLocation W43875260991 @default.
• W4387526099 hasOpenAccess W4387526099 @default.
• W4387526099 hasPrimaryLocation W43875260991 @default.
• W4387526099 hasRelatedWork W1494880142 @default.
• W4387526099 hasRelatedWork W1790425259 @default.
• W4387526099 hasRelatedWork W1978861814 @default.
• W4387526099 hasRelatedWork W2077587845 @default.

• next