Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387562938> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4387562938 endingPage "191" @default.
- W4387562938 startingPage "173" @default.
- W4387562938 abstract "Removal of the poly(A) tail, or deadenylation, is a crucial step in destabilizing mRNAs in eukaryotes. In this chapter, we describe a cell-free deadenylation assay that uses cytoplasmic cell extracts from human HEK293 cells transiently transfected with DNA encoding RNA-binding proteins (RBP), and in vitro-transcribed, radiolabeled, RNA probes. We include methods to evaluate the effects of RBPs or deadenylases on various in vitro-transcribed probes, with or without poly(A) tails. Finally, we also demonstrate the adaptability of these assays to test purified protein components in our cell-free deadenylation assay. In our experience, these methods are well suited for the initial assessment of the effects of RBPs on the deadenylation of mRNAs." @default.
- W4387562938 created "2023-10-13" @default.
- W4387562938 creator A5033396908 @default.
- W4387562938 creator A5058229432 @default.
- W4387562938 creator A5074341423 @default.
- W4387562938 date "2023-10-13" @default.
- W4387562938 modified "2023-10-13" @default.
- W4387562938 title "RNA-Binding Protein-Mediated mRNA Deadenylation in Mammalian Cell Extracts" @default.
- W4387562938 cites W1964532256 @default.
- W4387562938 cites W1971421846 @default.
- W4387562938 cites W1982263548 @default.
- W4387562938 cites W1998165054 @default.
- W4387562938 cites W2008746873 @default.
- W4387562938 cites W2018587522 @default.
- W4387562938 cites W2045714098 @default.
- W4387562938 cites W2052991419 @default.
- W4387562938 cites W2058220225 @default.
- W4387562938 cites W2125624608 @default.
- W4387562938 cites W2145989110 @default.
- W4387562938 cites W2164350157 @default.
- W4387562938 cites W2808950176 @default.
- W4387562938 doi "https://doi.org/10.1007/978-1-0716-3481-3_11" @default.
- W4387562938 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37824071" @default.
- W4387562938 hasPublicationYear "2023" @default.
- W4387562938 type Work @default.
- W4387562938 citedByCount "0" @default.
- W4387562938 crossrefType "book-chapter" @default.
- W4387562938 hasAuthorship W4387562938A5033396908 @default.
- W4387562938 hasAuthorship W4387562938A5058229432 @default.
- W4387562938 hasAuthorship W4387562938A5074341423 @default.
- W4387562938 hasConcept C104317684 @default.
- W4387562938 hasConcept C105580179 @default.
- W4387562938 hasConcept C1491633281 @default.
- W4387562938 hasConcept C153911025 @default.
- W4387562938 hasConcept C174510640 @default.
- W4387562938 hasConcept C185592680 @default.
- W4387562938 hasConcept C190062978 @default.
- W4387562938 hasConcept C41282012 @default.
- W4387562938 hasConcept C54009773 @default.
- W4387562938 hasConcept C54355233 @default.
- W4387562938 hasConcept C67705224 @default.
- W4387562938 hasConcept C81885089 @default.
- W4387562938 hasConcept C86803240 @default.
- W4387562938 hasConcept C95444343 @default.
- W4387562938 hasConceptScore W4387562938C104317684 @default.
- W4387562938 hasConceptScore W4387562938C105580179 @default.
- W4387562938 hasConceptScore W4387562938C1491633281 @default.
- W4387562938 hasConceptScore W4387562938C153911025 @default.
- W4387562938 hasConceptScore W4387562938C174510640 @default.
- W4387562938 hasConceptScore W4387562938C185592680 @default.
- W4387562938 hasConceptScore W4387562938C190062978 @default.
- W4387562938 hasConceptScore W4387562938C41282012 @default.
- W4387562938 hasConceptScore W4387562938C54009773 @default.
- W4387562938 hasConceptScore W4387562938C54355233 @default.
- W4387562938 hasConceptScore W4387562938C67705224 @default.
- W4387562938 hasConceptScore W4387562938C81885089 @default.
- W4387562938 hasConceptScore W4387562938C86803240 @default.
- W4387562938 hasConceptScore W4387562938C95444343 @default.
- W4387562938 hasLocation W43875629381 @default.
- W4387562938 hasLocation W43875629382 @default.
- W4387562938 hasOpenAccess W4387562938 @default.
- W4387562938 hasPrimaryLocation W43875629381 @default.
- W4387562938 hasRelatedWork W2024191897 @default.
- W4387562938 hasRelatedWork W2060189946 @default.
- W4387562938 hasRelatedWork W2077615126 @default.
- W4387562938 hasRelatedWork W2151252501 @default.
- W4387562938 hasRelatedWork W2354393567 @default.
- W4387562938 hasRelatedWork W2386445198 @default.
- W4387562938 hasRelatedWork W2389860412 @default.
- W4387562938 hasRelatedWork W2989893422 @default.
- W4387562938 hasRelatedWork W4210672545 @default.
- W4387562938 hasRelatedWork W4238547686 @default.
- W4387562938 isParatext "false" @default.
- W4387562938 isRetracted "false" @default.
- W4387562938 workType "book-chapter" @default.