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- W4387562972 abstract "A novel Gram-stain-positive bacterium, designated NB10 T , was isolated from the gut of Tenebrio molitor . The isolate was rod-shaped, aerobic, non-motile and non-spore-forming. Colonies of strain NB10 T were light yellow, circular and smooth. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that the isolate was related to the genu s Leucobacter . Its closest relatives were Leucobacter holotrichiae T14 T (97.8 % 16S rRNA gene sequence similarity), Leucobacter zeae CC-MF41 T (97.0%) and Leucobacter salsicius M1-8 T (96.4%). The DNA G+C content of strain NB10 T was 68.8 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization values among strain NB10 T and the selected Leucobacter species were ≤83.8 % (ANI- blast ), 87.6 % (ANI-MUMmer) and 29.6%, which were below the recommended cutoff values for species delineation. The predominant cellular fatty acids were anteiso-C 15 : 0 (39.0%), anteiso-C 17 : 0 (35.5%) and iso-C 16 : 0 (17.0%). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The predominant respiratory quinones were MK-11 and MK-10. The cell wall amino acids were Gly, Glu, Tr, Ala, and DAB. Based on these phylogenetic and phenotypic results, strain NB10 T can be clearly distinguished from all of the recognized species of the genus Leucobacter and is considered to represent a novel species of that genus. The name Leucobacter tenebrionis sp. nov. is proposed, with the type strain NB10 T (=MCCC 1K07072 T =KCTC 49728 T )." @default.
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- W4387562972 date "2023-10-12" @default.
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- W4387562972 title "Leucobacter tenebrionis sp. nov., isolated from the gut of Tenebrio molitor" @default.
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- W4387562972 doi "https://doi.org/10.1099/ijsem.0.006081" @default.
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