FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387563215> ?p ?o ?g. }

• W4387563215 abstract "In hollow visceral organs, capillary pericytes appear to drive spontaneous Ca2+ transients in the upstream arterioles. Here, mechanisms underlying the intercellular synchrony of pericyte Ca2+ transients were explored. Ca2+ dynamics in NG2 chondroitin sulphate proteoglycan (NG2)-expressing capillary pericytes were examined using rectal mucosa-submucosa preparations of NG2-GCaMP6 mice. Spontaneous Ca2+ transients arising from endoplasmic reticulum Ca2+ release were synchronously developed amongst capillary pericytes in a gap junction blocker (3 μM carbenoxolone)-sensitive manner and could spread into upstream vascular segments. Spontaneous Ca2+ transients were suppressed by the Ca2+ -activated Cl- channel (CaCC) blocker niflumic acid and their synchrony was diminished by a TMEM16A inhibitor (3 μM Ani9) in accordance with TMEM16A immunoreactivity in pericytes. In capillaries where cyclooxygenase (COX)-2 immunoreactivity was expressed in endothelium but not pericytes, non-selective COX inhibitors (1 μM indomethacin or 10 μM diclofenac) or COX-2 inhibitor (10 μM NS 398) disrupted the synchrony of spontaneous Ca2+ transients and raised the basal Ca2+ level. Subsequent prostaglandin I2 (PGI2 ; 100 nM) or the KATP channel opener levcromakalim restored the synchrony with a reduction in the Ca2+ level. PGI2 receptor antagonist (1 μM RO1138452) also disrupted the synchrony of spontaneous Ca2+ transients and increased the basal Ca2+ level. Subsequent levcromakalim restored the synchrony and reversed the Ca2+ rise. Thus, the synchrony of spontaneous Ca2+ transients in pericytes appears to be developed by the spread of spontaneous transient depolarisations arising from the opening of TMEM16A CaCCs. Endothelial PGI2 may play a role in maintaining the synchrony, presumably by stabilising the resting membrane potential in pericytes. KEY POINTS: Capillary pericytes in the rectal mucosa generate synchronous spontaneous Ca2+ transients that could spread into the upstream vascular segment. Spontaneous Ca2+ release from the endoplasmic reticulum (ER) triggers the opening of Ca2+ -activated Cl- channel TMEM16A and resultant depolarisations that spread amongst pericytes via gap junctions, establishing the synchrony of spontaneous Ca2+ transients in pericytes. Prostaglandin I2 (PGI2 ), which is constitutively produced by the endothelium depending on cyclooxygenase-2, appears to prevent premature ER Ca2+ releases in the pericytes allowing periodic, regenerative Ca2+ releases. Endothelial PGI2 may maintain the synchrony of pericyte activity by stabilising pericyte resting membrane potential by opening of KATP channels." @default.
• W4387563215 created "2023-10-13" @default.
• W4387563215 creator A5006604957 @default.
• W4387563215 creator A5062385448 @default.
• W4387563215 creator A5063062046 @default.
• W4387563215 date "2023-10-11" @default.
• W4387563215 modified "2023-10-13" @default.
• W4387563215 title "Roles of endothelial prostaglandin I₂ in maintaining synchronous spontaneous Ca²⁺ transients in rectal capillary pericytes" @default.
• W4387563215 cites W1540025214 @default.
• W4387563215 cites W1545209966 @default.
• W4387563215 cites W1545464595 @default.
• W4387563215 cites W1587572153 @default.
• W4387563215 cites W1731997554 @default.
• W4387563215 cites W1940693565 @default.
• W4387563215 cites W1976101303 @default.
• W4387563215 cites W1982580263 @default.
• W4387563215 cites W1983988297 @default.
• W4387563215 cites W1989283313 @default.
• W4387563215 cites W1994497806 @default.
• W4387563215 cites W2003971416 @default.
• W4387563215 cites W2005402925 @default.
• W4387563215 cites W2012798948 @default.
• W4387563215 cites W2018550116 @default.
• W4387563215 cites W2024487247 @default.
• W4387563215 cites W2032770343 @default.
• W4387563215 cites W2034182021 @default.
• W4387563215 cites W2037490133 @default.
• W4387563215 cites W2040909007 @default.
• W4387563215 cites W2041249812 @default.
• W4387563215 cites W2048148521 @default.
• W4387563215 cites W2053277906 @default.
• W4387563215 cites W2058360584 @default.
• W4387563215 cites W2058561120 @default.
• W4387563215 cites W2067474104 @default.
• W4387563215 cites W2069401428 @default.
• W4387563215 cites W2069929255 @default.
• W4387563215 cites W2085335942 @default.
• W4387563215 cites W2089234699 @default.
• W4387563215 cites W2092109656 @default.
• W4387563215 cites W2103279601 @default.
• W4387563215 cites W2107108081 @default.
• W4387563215 cites W2113568840 @default.
• W4387563215 cites W2118697373 @default.
• W4387563215 cites W2122283496 @default.
• W4387563215 cites W2131008034 @default.
• W4387563215 cites W2131472180 @default.
• W4387563215 cites W2144821133 @default.
• W4387563215 cites W2146403616 @default.
• W4387563215 cites W2152827122 @default.
• W4387563215 cites W2189452613 @default.
• W4387563215 cites W2229160604 @default.
• W4387563215 cites W2317177510 @default.
• W4387563215 cites W2396098376 @default.
• W4387563215 cites W2605536235 @default.
• W4387563215 cites W2772755903 @default.
• W4387563215 cites W2792281792 @default.
• W4387563215 cites W2800607287 @default.
• W4387563215 cites W2888244399 @default.
• W4387563215 cites W2892062896 @default.
• W4387563215 cites W2938252840 @default.
• W4387563215 cites W3014947556 @default.
• W4387563215 cites W3112435596 @default.
• W4387563215 cites W3164600279 @default.
• W4387563215 cites W4225312023 @default.
• W4387563215 cites W4225587542 @default.
• W4387563215 cites W4310219792 @default.
• W4387563215 doi "https://doi.org/10.1113/jp284284" @default.
• W4387563215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37819628" @default.
• W4387563215 hasPublicationYear "2023" @default.
• W4387563215 type Work @default.
• W4387563215 citedByCount "0" @default.
• W4387563215 crossrefType "journal-article" @default.
• W4387563215 hasAuthorship W4387563215A5006604957 @default.
• W4387563215 hasAuthorship W4387563215A5062385448 @default.
• W4387563215 hasAuthorship W4387563215A5063062046 @default.
• W4387563215 hasBestOaLocation W43875632151 @default.
• W4387563215 hasConcept C123012128 @default.
• W4387563215 hasConcept C12554922 @default.
• W4387563215 hasConcept C126322002 @default.
• W4387563215 hasConcept C134018914 @default.
• W4387563215 hasConcept C155164980 @default.
• W4387563215 hasConcept C158157758 @default.
• W4387563215 hasConcept C158617107 @default.
• W4387563215 hasConcept C170488646 @default.
• W4387563215 hasConcept C170493617 @default.
• W4387563215 hasConcept C181199279 @default.
• W4387563215 hasConcept C185592680 @default.
• W4387563215 hasConcept C202751555 @default.
• W4387563215 hasConcept C2779689624 @default.
• W4387563215 hasConcept C2779700409 @default.
• W4387563215 hasConcept C2779996123 @default.
• W4387563215 hasConcept C2780383360 @default.
• W4387563215 hasConcept C2780427682 @default.
• W4387563215 hasConcept C2780664492 @default.
• W4387563215 hasConcept C2780961449 @default.
• W4387563215 hasConcept C55493867 @default.
• W4387563215 hasConcept C71924100 @default.
• W4387563215 hasConcept C79879829 @default.
• W4387563215 hasConcept C86803240 @default.
• W4387563215 hasConcept C95444343 @default.

• next