Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387564967> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W4387564967 abstract "The in vitro production and cryopreservation of mammalian embryos generate reactive oxygen species (ROS) due to conditions of the system that can overcome their antioxidant protection. Resveratrol is an antioxidant used in in vitro systems improving blastocyst rates, but its effect on antioxidant enzymes such as glutathione (GSH) in embryos produced by in vitro fertilization (IVF) after vitrification has not been reported. The objective of this study was to evaluate the effect of resveratrol in the in vitro maturation medium (IVM) of sheep oocytes (Ovis aries) on the levels of ROS and GSH in embryos produced by IVF subjected to vitrification. Resveratrol was added at 0µM, 0.25µM, 0.5µM and 1µM during oocyte in vitro maturation (IVM). Matured oocytes were fertilized with thawed ram sperm. Embryos were cultured in sequential media until blastocysts, then were vitrified for 24 h and after heating they were stained with DCFH-DA (2',7'-dichlorodihydrofluorescein diacetate) to determine the presence of ROS and with Cell Tracker Blue® for the presence of GSH. The quantitative values of ROS and GSH were obtained through the Image J image processor. The results showed that resveratrol increases GSH and decreases ROS production (p<0.05). It is concluded that its use in sheep oocytes during IVM has a beneficial effect on embryos produced by IVF subjected to vitrification, protecting them from oxidative damage by promoting the synthesis of antioxidants." @default.
- W4387564967 created "2023-10-13" @default.
- W4387564967 creator A5000334447 @default.
- W4387564967 creator A5006964008 @default.
- W4387564967 creator A5012488719 @default.
- W4387564967 creator A5017330669 @default.
- W4387564967 creator A5045873582 @default.
- W4387564967 creator A5057855371 @default.
- W4387564967 creator A5061110699 @default.
- W4387564967 creator A5093048896 @default.
- W4387564967 date "2023-10-06" @default.
- W4387564967 modified "2023-10-18" @default.
- W4387564967 title "Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos" @default.
- W4387564967 doi "https://doi.org/10.20944/preprints202310.0303.v1" @default.
- W4387564967 hasPublicationYear "2023" @default.
- W4387564967 type Work @default.
- W4387564967 citedByCount "0" @default.
- W4387564967 crossrefType "posted-content" @default.
- W4387564967 hasAuthorship W4387564967A5000334447 @default.
- W4387564967 hasAuthorship W4387564967A5006964008 @default.
- W4387564967 hasAuthorship W4387564967A5012488719 @default.
- W4387564967 hasAuthorship W4387564967A5017330669 @default.
- W4387564967 hasAuthorship W4387564967A5045873582 @default.
- W4387564967 hasAuthorship W4387564967A5057855371 @default.
- W4387564967 hasAuthorship W4387564967A5061110699 @default.
- W4387564967 hasAuthorship W4387564967A5093048896 @default.
- W4387564967 hasBestOaLocation W43875649671 @default.
- W4387564967 hasConcept C105702510 @default.
- W4387564967 hasConcept C16685009 @default.
- W4387564967 hasConcept C179933525 @default.
- W4387564967 hasConcept C181199279 @default.
- W4387564967 hasConcept C185592680 @default.
- W4387564967 hasConcept C196843134 @default.
- W4387564967 hasConcept C2775966511 @default.
- W4387564967 hasConcept C2776690073 @default.
- W4387564967 hasConcept C2776816829 @default.
- W4387564967 hasConcept C2778004101 @default.
- W4387564967 hasConcept C2778022349 @default.
- W4387564967 hasConcept C2778177303 @default.
- W4387564967 hasConcept C48349386 @default.
- W4387564967 hasConcept C538909803 @default.
- W4387564967 hasConcept C55493867 @default.
- W4387564967 hasConcept C71924100 @default.
- W4387564967 hasConcept C86803240 @default.
- W4387564967 hasConcept C87073359 @default.
- W4387564967 hasConcept C88972607 @default.
- W4387564967 hasConcept C95444343 @default.
- W4387564967 hasConceptScore W4387564967C105702510 @default.
- W4387564967 hasConceptScore W4387564967C16685009 @default.
- W4387564967 hasConceptScore W4387564967C179933525 @default.
- W4387564967 hasConceptScore W4387564967C181199279 @default.
- W4387564967 hasConceptScore W4387564967C185592680 @default.
- W4387564967 hasConceptScore W4387564967C196843134 @default.
- W4387564967 hasConceptScore W4387564967C2775966511 @default.
- W4387564967 hasConceptScore W4387564967C2776690073 @default.
- W4387564967 hasConceptScore W4387564967C2776816829 @default.
- W4387564967 hasConceptScore W4387564967C2778004101 @default.
- W4387564967 hasConceptScore W4387564967C2778022349 @default.
- W4387564967 hasConceptScore W4387564967C2778177303 @default.
- W4387564967 hasConceptScore W4387564967C48349386 @default.
- W4387564967 hasConceptScore W4387564967C538909803 @default.
- W4387564967 hasConceptScore W4387564967C55493867 @default.
- W4387564967 hasConceptScore W4387564967C71924100 @default.
- W4387564967 hasConceptScore W4387564967C86803240 @default.
- W4387564967 hasConceptScore W4387564967C87073359 @default.
- W4387564967 hasConceptScore W4387564967C88972607 @default.
- W4387564967 hasConceptScore W4387564967C95444343 @default.
- W4387564967 hasLocation W43875649671 @default.
- W4387564967 hasOpenAccess W4387564967 @default.
- W4387564967 hasPrimaryLocation W43875649671 @default.
- W4387564967 hasRelatedWork W139959371 @default.
- W4387564967 hasRelatedWork W17480179 @default.
- W4387564967 hasRelatedWork W2348639946 @default.
- W4387564967 hasRelatedWork W2371008047 @default.
- W4387564967 hasRelatedWork W2375433245 @default.
- W4387564967 hasRelatedWork W2802621196 @default.
- W4387564967 hasRelatedWork W2902119742 @default.
- W4387564967 hasRelatedWork W2965225922 @default.
- W4387564967 hasRelatedWork W4220983607 @default.
- W4387564967 hasRelatedWork W4229376922 @default.
- W4387564967 isParatext "false" @default.
- W4387564967 isRetracted "false" @default.
- W4387564967 workType "article" @default.