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- W4387568263 abstract "Tryptophan synthase catalyzes the synthesis of a wide array of non-canonical amino acids and is an attractive target for directed evolution. Droplet microfluidics offers an ultrahigh throughput approach to directed evolution (>107 experiments per day), enabling the search for biocatalysts in wider regions of sequence space with reagent consumption minimized to the picoliter volume (per library member). While the majority of screening campaigns in this format on record relied on an optically active reaction product, a new assay is needed for tryptophan synthase. Tryptophan is not fluorogenic in the visible light spectrum and thus falls outside the scope of conventional droplet microfluidic read-outs which are incompatible with UV light detection at high throughput. Here, we engineer a tryptophan DNA aptamer into a biosensor to quantitatively report on tryptophan production in droplets. The utility of the biosensor was validated by identifying 5-fold improved tryptophan synthases from ~100,000 protein variants. More generally this work establishes the use of DNA-aptamer sensors with a fluorogenic read-out in widening the scope of droplet microfluidic evolution." @default.
- W4387568263 created "2023-10-13" @default.
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- W4387568263 date "2023-10-12" @default.
- W4387568263 modified "2023-10-13" @default.
- W4387568263 title "Ultrahigh throughput evolution of tryptophan synthase in droplets via an aptamer-biosensor" @default.
- W4387568263 doi "https://doi.org/10.1101/2023.10.11.561886" @default.
- W4387568263 hasPublicationYear "2023" @default.
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