Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387576260> ?p ?o ?g. }
- W4387576260 abstract "Abstract The emergence of thiol‐mediated uptake (TMU) as a powerful strategy to penetrate cells calls for the development of practical small‐molecule TMU tools for traceless delivery. Toward this goal, esters are explored here as bioreversible linkers between dynamic covalent cascade exchangers accounting for TMU and the protein of interest (POI). The method relies on α ‐aryl‐ α ‐diazoamides that react with carboxylic acids of the POI to form esters that can be enzymatically hydrolyzed inside cells to release the native POI. A two‐step protocol is established for bioreversible conjugation of TMU tags to the POI. Despite the small number of tags attached to POIs to prevent isoelectric precipitation, POIs with traceless TMU tags are shown to efficiently enter cells not only in 2D culture but also in 3D spheroids mimicking deep tissue, confirming a key advantage of TMU. Uptake inhibition by various thiol‐reactive agents confirms the participation of cell‐surface thiols in cell penetration, i. e., the occurrence of TMU." @default.
- W4387576260 created "2023-10-13" @default.
- W4387576260 creator A5012111264 @default.
- W4387576260 creator A5037475806 @default.
- W4387576260 creator A5037906302 @default.
- W4387576260 creator A5045693449 @default.
- W4387576260 creator A5066285072 @default.
- W4387576260 creator A5070791350 @default.
- W4387576260 creator A5078354341 @default.
- W4387576260 creator A5088737577 @default.
- W4387576260 date "2023-10-11" @default.
- W4387576260 modified "2023-10-14" @default.
- W4387576260 title "Toward a Traceless Tag for the Thiol‐Mediated Uptake of Proteins" @default.
- W4387576260 cites W1945627170 @default.
- W4387576260 cites W1967847890 @default.
- W4387576260 cites W1973671987 @default.
- W4387576260 cites W2029697829 @default.
- W4387576260 cites W2049307465 @default.
- W4387576260 cites W2065135892 @default.
- W4387576260 cites W2065310958 @default.
- W4387576260 cites W2068130700 @default.
- W4387576260 cites W2070521942 @default.
- W4387576260 cites W2083663368 @default.
- W4387576260 cites W2085065303 @default.
- W4387576260 cites W2092716475 @default.
- W4387576260 cites W2121588632 @default.
- W4387576260 cites W2154490707 @default.
- W4387576260 cites W2322836762 @default.
- W4387576260 cites W2340128003 @default.
- W4387576260 cites W2343973808 @default.
- W4387576260 cites W2560459299 @default.
- W4387576260 cites W2603101815 @default.
- W4387576260 cites W2603915256 @default.
- W4387576260 cites W2731703102 @default.
- W4387576260 cites W2763313852 @default.
- W4387576260 cites W2894606879 @default.
- W4387576260 cites W2898750930 @default.
- W4387576260 cites W2911342504 @default.
- W4387576260 cites W2913184811 @default.
- W4387576260 cites W2916045020 @default.
- W4387576260 cites W2919253225 @default.
- W4387576260 cites W2924100215 @default.
- W4387576260 cites W2947611835 @default.
- W4387576260 cites W2969589527 @default.
- W4387576260 cites W3000454051 @default.
- W4387576260 cites W3003992713 @default.
- W4387576260 cites W3008964884 @default.
- W4387576260 cites W3023423471 @default.
- W4387576260 cites W3026690957 @default.
- W4387576260 cites W3042902202 @default.
- W4387576260 cites W3048827115 @default.
- W4387576260 cites W3104991008 @default.
- W4387576260 cites W3107988949 @default.
- W4387576260 cites W3159607027 @default.
- W4387576260 cites W3170478534 @default.
- W4387576260 cites W3175238357 @default.
- W4387576260 cites W3178724938 @default.
- W4387576260 cites W3203336166 @default.
- W4387576260 cites W3206155617 @default.
- W4387576260 cites W3216602255 @default.
- W4387576260 cites W4220709766 @default.
- W4387576260 cites W4220735519 @default.
- W4387576260 cites W4220958293 @default.
- W4387576260 cites W4281719761 @default.
- W4387576260 cites W4282922004 @default.
- W4387576260 cites W4283714429 @default.
- W4387576260 cites W4286560522 @default.
- W4387576260 cites W4293456941 @default.
- W4387576260 cites W4306411189 @default.
- W4387576260 cites W4307115544 @default.
- W4387576260 cites W4310943225 @default.
- W4387576260 cites W4311820931 @default.
- W4387576260 cites W4321365713 @default.
- W4387576260 cites W4322615342 @default.
- W4387576260 cites W4324303478 @default.
- W4387576260 cites W4360609736 @default.
- W4387576260 cites W4360946223 @default.
- W4387576260 cites W4365140075 @default.
- W4387576260 cites W4367040511 @default.
- W4387576260 doi "https://doi.org/10.1002/ceur.202300029" @default.
- W4387576260 hasPublicationYear "2023" @default.
- W4387576260 type Work @default.
- W4387576260 citedByCount "0" @default.
- W4387576260 crossrefType "journal-article" @default.
- W4387576260 hasAuthorship W4387576260A5012111264 @default.
- W4387576260 hasAuthorship W4387576260A5037475806 @default.
- W4387576260 hasAuthorship W4387576260A5037906302 @default.
- W4387576260 hasAuthorship W4387576260A5045693449 @default.
- W4387576260 hasAuthorship W4387576260A5066285072 @default.
- W4387576260 hasAuthorship W4387576260A5070791350 @default.
- W4387576260 hasAuthorship W4387576260A5078354341 @default.
- W4387576260 hasAuthorship W4387576260A5088737577 @default.
- W4387576260 hasBestOaLocation W43875762601 @default.
- W4387576260 hasConcept C1491633281 @default.
- W4387576260 hasConcept C161624437 @default.
- W4387576260 hasConcept C181199279 @default.
- W4387576260 hasConcept C185592680 @default.
- W4387576260 hasConcept C21951064 @default.
- W4387576260 hasConcept C2781324293 @default.
- W4387576260 hasConcept C50476208 @default.