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- W4387598438 abstract "Lysine acetylation (KAc) is a reversible post-translational modification (PTM) that can alter protein structure and function; however, specific roles for KAc are largely undefined in bacteria. Acetyl-lysine immunoprecipitation and LC-MS/MS identified 5567 acetylated lysines on 1026 proteins from the gastrointestinal pathogen Campylobacter jejuni (∼63% of the predicted proteome). KAc was identified on proteins from all subcellular locations, including the outer membrane (OM) and extracellular proteins. Label-based LC-MS/MS identified proteins and KAc sites during growth in 0.1% sodium deoxycholate (DOC, a component of gut bile salts). 3410 acetylated peptides were quantified, and 784 (from 409 proteins) were differentially abundant in DOC growth. Changes in KAc involved multiple pathways, suggesting a dynamic role for this PTM in bile resistance. As observed elsewhere, we show KAc is primarily nonenzymatically mediated via acetyl-phosphate; however, the deacetylase CobB also contributes to a global elevation of this modification in DOC. We observed several multiply acetylated OM proteins and altered DOC abundance of acetylated peptides in the fibronectin (Fn)-binding adhesin CadF. We show KAc reduces CadF Fn binding and prevalence of lower mass variants. This study provides the first system-wide lysine acetylome of C. jejuni and contributes to our understanding of KAc as an emerging PTM in bacteria." @default.
- W4387598438 created "2023-10-14" @default.
- W4387598438 creator A5009846378 @default.
- W4387598438 creator A5031503282 @default.
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- W4387598438 date "2023-10-13" @default.
- W4387598438 modified "2023-10-14" @default.
- W4387598438 title "Global Acetylomics of <i>Campylobacter jejuni</i> Shows Lysine Acetylation Regulates CadF Adhesin Processing and Human Fibronectin Binding" @default.
- W4387598438 cites W1511815964 @default.
- W4387598438 cites W1849301422 @default.
- W4387598438 cites W1899091258 @default.
- W4387598438 cites W1963454015 @default.
- W4387598438 cites W1974403675 @default.
- W4387598438 cites W1975692560 @default.
- W4387598438 cites W1979751618 @default.
- W4387598438 cites W1981153918 @default.
- W4387598438 cites W1987547306 @default.
- W4387598438 cites W1995572199 @default.
- W4387598438 cites W1998823095 @default.
- W4387598438 cites W1999342657 @default.
- W4387598438 cites W2009974397 @default.
- W4387598438 cites W2011970304 @default.
- W4387598438 cites W2016229097 @default.
- W4387598438 cites W2026465178 @default.
- W4387598438 cites W2033608667 @default.
- W4387598438 cites W2034614265 @default.
- W4387598438 cites W2035911064 @default.
- W4387598438 cites W2039071378 @default.
- W4387598438 cites W2044201211 @default.
- W4387598438 cites W2051152566 @default.
- W4387598438 cites W2064921878 @default.
- W4387598438 cites W2076165765 @default.
- W4387598438 cites W2083078738 @default.
- W4387598438 cites W2086779879 @default.
- W4387598438 cites W2091037376 @default.
- W4387598438 cites W2094685393 @default.
- W4387598438 cites W2113394693 @default.
- W4387598438 cites W2114346444 @default.
- W4387598438 cites W2120904752 @default.
- W4387598438 cites W2124869787 @default.
- W4387598438 cites W2128981277 @default.
- W4387598438 cites W2131233236 @default.
- W4387598438 cites W2134809564 @default.
- W4387598438 cites W2136773773 @default.
- W4387598438 cites W2140590690 @default.
- W4387598438 cites W2141445066 @default.
- W4387598438 cites W2142342668 @default.
- W4387598438 cites W2144495216 @default.
- W4387598438 cites W2144529284 @default.
- W4387598438 cites W2146766478 @default.
- W4387598438 cites W2156512781 @default.
- W4387598438 cites W2158217645 @default.
- W4387598438 cites W2159276924 @default.
- W4387598438 cites W2159556209 @default.
- W4387598438 cites W2160235203 @default.
- W4387598438 cites W2161025101 @default.
- W4387598438 cites W2161725470 @default.
- W4387598438 cites W2162904770 @default.
- W4387598438 cites W2279619472 @default.
- W4387598438 cites W2408866688 @default.
- W4387598438 cites W2463195069 @default.
- W4387598438 cites W2545827800 @default.
- W4387598438 cites W2590518997 @default.
- W4387598438 cites W2591753289 @default.
- W4387598438 cites W2765592108 @default.
- W4387598438 cites W2765663455 @default.
- W4387598438 cites W2767867833 @default.
- W4387598438 cites W2770660239 @default.
- W4387598438 cites W2777140576 @default.
- W4387598438 cites W2794342080 @default.
- W4387598438 cites W2804766030 @default.
- W4387598438 cites W2804977237 @default.
- W4387598438 cites W2892689024 @default.
- W4387598438 cites W2898854709 @default.
- W4387598438 cites W2900569176 @default.
- W4387598438 cites W2900808325 @default.
- W4387598438 cites W2909041805 @default.
- W4387598438 cites W2910124514 @default.
- W4387598438 cites W2938882297 @default.
- W4387598438 cites W2945386484 @default.
- W4387598438 cites W2950243974 @default.
- W4387598438 cites W2957950506 @default.
- W4387598438 cites W2967487752 @default.
- W4387598438 cites W3020253161 @default.
- W4387598438 cites W3021442976 @default.
- W4387598438 cites W3101195279 @default.
- W4387598438 cites W3111070972 @default.
- W4387598438 cites W3134613184 @default.
- W4387598438 cites W3173569684 @default.
- W4387598438 cites W3185823781 @default.
- W4387598438 cites W3193293791 @default.
- W4387598438 cites W3208691473 @default.
- W4387598438 cites W3209976162 @default.
- W4387598438 cites W3216736125 @default.
- W4387598438 cites W4206484481 @default.
- W4387598438 cites W4281254333 @default.
- W4387598438 cites W4293762885 @default.
- W4387598438 cites W4298145096 @default.
- W4387598438 cites W4318165230 @default.
- W4387598438 cites W805633396 @default.