FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387599769> ?p ?o ?g. }

• W4387599769 abstract "Staphylococcus aureus is a clinically important pathogen that threatens human health due to its strong pathogenicity and drug resistance, leading to meningitis, endocarditis, and skin and soft tissue infections. Genetic manipulation in S. aureus is a powerful approach for characterizing the molecular mechanisms of bacterial drug resistance, pathogenicity, and virulence. However, a strong restriction barrier presents a major obstacle to the extensive utilization of genetic manipulation tools in clinical isolates of S. aureus. Here, we constructed a restriction-modification (RM) system silent CRISPR-Cas9 toolkit that synonymously eliminated the type I RM targets of S. aureus from plasmids, downsized plasmids using minicircle technology, and combined with a plasmid artificial modification (PAM) method to circumvent the type II RM system. The RM-silent CRISPR-Cas9 toolkit enables a significant improvement in transformation (105-106 transformants per microgram plasmid in strains we tested) and high-success efficiency editing for gene deletion (knockout strain obtained in one-round electroporation) in a wide range of S. aureus species including clinical isolates of unknown genetic background. The RM-silent CRISPR-Cas9 toolkits could expedite the process of mutant construction in most S. aureus strains, and this approach could be applied to the design of other genetic toolkit plasmids for utilization in a wider range of S. aureus strains." @default.
• W4387599769 created "2023-10-14" @default.
• W4387599769 creator A5011639911 @default.
• W4387599769 creator A5047438925 @default.
• W4387599769 creator A5050008056 @default.
• W4387599769 creator A5050530612 @default.
• W4387599769 creator A5075394139 @default.
• W4387599769 creator A5085028455 @default.
• W4387599769 creator A5085160180 @default.
• W4387599769 date "2023-10-13" @default.
• W4387599769 modified "2023-10-14" @default.
• W4387599769 title "Efficient Genome Editing in Most <i>Staphylococcus aureus</i> by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit" @default.
• W4387599769 cites W1494068198 @default.
• W4387599769 cites W1516313529 @default.
• W4387599769 cites W1878404532 @default.
• W4387599769 cites W1965772100 @default.
• W4387599769 cites W2003484929 @default.
• W4387599769 cites W2024110190 @default.
• W4387599769 cites W2042935657 @default.
• W4387599769 cites W2042956661 @default.
• W4387599769 cites W2048628486 @default.
• W4387599769 cites W2079903460 @default.
• W4387599769 cites W2081516250 @default.
• W4387599769 cites W2082324448 @default.
• W4387599769 cites W2090264984 @default.
• W4387599769 cites W2108828818 @default.
• W4387599769 cites W2110763275 @default.
• W4387599769 cites W2122020611 @default.
• W4387599769 cites W2126521696 @default.
• W4387599769 cites W2130920842 @default.
• W4387599769 cites W2152900584 @default.
• W4387599769 cites W2156738105 @default.
• W4387599769 cites W2169614539 @default.
• W4387599769 cites W2170367123 @default.
• W4387599769 cites W2173737475 @default.
• W4387599769 cites W2252568502 @default.
• W4387599769 cites W2312720395 @default.
• W4387599769 cites W2314649741 @default.
• W4387599769 cites W2413011000 @default.
• W4387599769 cites W2586226593 @default.
• W4387599769 cites W2590355459 @default.
• W4387599769 cites W2737490233 @default.
• W4387599769 cites W2781931843 @default.
• W4387599769 cites W2793296961 @default.
• W4387599769 cites W2944902712 @default.
• W4387599769 cites W2996552947 @default.
• W4387599769 cites W3038956601 @default.
• W4387599769 cites W3097198294 @default.
• W4387599769 cites W3126260617 @default.
• W4387599769 cites W3136194665 @default.
• W4387599769 cites W4236815367 @default.
• W4387599769 cites W4292952487 @default.
• W4387599769 cites W4307838425 @default.
• W4387599769 cites W76823895 @default.
• W4387599769 doi "https://doi.org/10.1021/acssynbio.3c00339" @default.
• W4387599769 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37830328" @default.
• W4387599769 hasPublicationYear "2023" @default.
• W4387599769 type Work @default.
• W4387599769 citedByCount "0" @default.
• W4387599769 crossrefType "journal-article" @default.
• W4387599769 hasAuthorship W4387599769A5011639911 @default.
• W4387599769 hasAuthorship W4387599769A5047438925 @default.
• W4387599769 hasAuthorship W4387599769A5050008056 @default.
• W4387599769 hasAuthorship W4387599769A5050530612 @default.
• W4387599769 hasAuthorship W4387599769A5075394139 @default.
• W4387599769 hasAuthorship W4387599769A5085028455 @default.
• W4387599769 hasAuthorship W4387599769A5085160180 @default.
• W4387599769 hasConcept C104317684 @default.
• W4387599769 hasConcept C132455925 @default.
• W4387599769 hasConcept C141231307 @default.
• W4387599769 hasConcept C144501496 @default.
• W4387599769 hasConcept C186852380 @default.
• W4387599769 hasConcept C22744801 @default.
• W4387599769 hasConcept C2779489039 @default.
• W4387599769 hasConcept C523546767 @default.
• W4387599769 hasConcept C54355233 @default.
• W4387599769 hasConcept C60987743 @default.
• W4387599769 hasConcept C70721500 @default.
• W4387599769 hasConcept C86803240 @default.
• W4387599769 hasConcept C89423630 @default.
• W4387599769 hasConcept C98108389 @default.
• W4387599769 hasConceptScore W4387599769C104317684 @default.
• W4387599769 hasConceptScore W4387599769C132455925 @default.
• W4387599769 hasConceptScore W4387599769C141231307 @default.
• W4387599769 hasConceptScore W4387599769C144501496 @default.
• W4387599769 hasConceptScore W4387599769C186852380 @default.
• W4387599769 hasConceptScore W4387599769C22744801 @default.
• W4387599769 hasConceptScore W4387599769C2779489039 @default.
• W4387599769 hasConceptScore W4387599769C523546767 @default.
• W4387599769 hasConceptScore W4387599769C54355233 @default.
• W4387599769 hasConceptScore W4387599769C60987743 @default.
• W4387599769 hasConceptScore W4387599769C70721500 @default.
• W4387599769 hasConceptScore W4387599769C86803240 @default.
• W4387599769 hasConceptScore W4387599769C89423630 @default.
• W4387599769 hasConceptScore W4387599769C98108389 @default.
• W4387599769 hasFunder F4320321001 @default.
• W4387599769 hasLocation W43875997691 @default.
• W4387599769 hasLocation W43875997692 @default.
• W4387599769 hasOpenAccess W4387599769 @default.
• W4387599769 hasPrimaryLocation W43875997691 @default.

• next