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- W4387605086 abstract "Cellular redox state determinants are traditionally studied using fluorescent microscopy and immunoblot analysis; however, no procedure has been developed for simultaneous measurement in various immune cell subsets. Here, we present a flow cytometry assay for measuring antioxidant defense systems and reactive oxygen species simultaneously in T, B, and natural killer lymphocytes. We describe steps for preparing and treating peripheral blood mononuclear cells, surface and dye staining, cell fixation/permeabilization, and intracellular staining. We then detail machine standardization, acquisition, and analysis." @default.
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- W4387605086 date "2023-12-01" @default.
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- W4387605086 title "A redox-based characterization of human immune cell subsets by polychromatic flow cytometry" @default.
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- W4387605086 doi "https://doi.org/10.1016/j.xpro.2023.102632" @default.
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