Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387605344> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W4387605344 abstract "Acrosome exocytosis (AE), in which the sperm's single exocytotic vesicle fuses with the plasma membrane, is a complex, calcium-dependent process essential for fertilization. However, our understanding of how calcium signaling regulates AE is still incomplete. In particular, the interplay between intra-acrosomal calcium dynamics and the intermediate steps leading to AE is not well-defined. Here, we describe a method that provides spatial and temporal insights into acrosomal calcium dynamics and their relationship to membrane fusion and subsequent exocytosis of the acrosome vesicle. The method utilizes a novel transgenic mouse expressing an Acrosome-targeted Sensor for Exocytosis (AcroSensE). The sensor combines a genetically encoded calcium indicator (GCaMP) fused with mCherry. This fusion protein was specifically designed to enable the concurrent observation of acrosomal calcium dynamics and membrane fusion events. Real-time monitoring of acrosomal calcium dynamics and AE in live AcroSensE sperm is achieved using a combination of high frame-rate imaging and a stimulant delivery system that can target single sperm. This protocol also provides several examples of basic methods to quantify and analyze the raw data. Because the AcroSensE model is genetically encoded, its scientific significance can be augmented by using readily available genetic tools, such as crossbreeding with other mouse genetic models or gene-editing (CRISPR) based methods. With this strategy, the roles of additional signaling pathways in sperm capacitation and fertilization can be resolved. In summary, the method described here provides a convenient and effective tool to study calcium dynamics in a specific subcellular compartment-the sperm acrosome-and how those dynamics regulate the intermediate steps leading to membrane fusion and acrosome exocytosis." @default.
- W4387605344 created "2023-10-14" @default.
- W4387605344 creator A5007598622 @default.
- W4387605344 creator A5033417502 @default.
- W4387605344 creator A5064475877 @default.
- W4387605344 creator A5066881844 @default.
- W4387605344 creator A5077520441 @default.
- W4387605344 creator A5078972479 @default.
- W4387605344 date "2023-10-13" @default.
- W4387605344 modified "2023-10-14" @default.
- W4387605344 title "Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm" @default.
- W4387605344 doi "https://doi.org/10.3791/65962" @default.
- W4387605344 hasPublicationYear "2023" @default.
- W4387605344 type Work @default.
- W4387605344 citedByCount "0" @default.
- W4387605344 crossrefType "journal-article" @default.
- W4387605344 hasAuthorship W4387605344A5007598622 @default.
- W4387605344 hasAuthorship W4387605344A5033417502 @default.
- W4387605344 hasAuthorship W4387605344A5064475877 @default.
- W4387605344 hasAuthorship W4387605344A5066881844 @default.
- W4387605344 hasAuthorship W4387605344A5077520441 @default.
- W4387605344 hasAuthorship W4387605344A5078972479 @default.
- W4387605344 hasConcept C103038307 @default.
- W4387605344 hasConcept C175969161 @default.
- W4387605344 hasConcept C178790620 @default.
- W4387605344 hasConcept C185592680 @default.
- W4387605344 hasConcept C201571599 @default.
- W4387605344 hasConcept C2778252442 @default.
- W4387605344 hasConcept C2779995066 @default.
- W4387605344 hasConcept C2780301668 @default.
- W4387605344 hasConcept C2781087480 @default.
- W4387605344 hasConcept C41625074 @default.
- W4387605344 hasConcept C519063684 @default.
- W4387605344 hasConcept C54355233 @default.
- W4387605344 hasConcept C62478195 @default.
- W4387605344 hasConcept C86803240 @default.
- W4387605344 hasConcept C95444343 @default.
- W4387605344 hasConceptScore W4387605344C103038307 @default.
- W4387605344 hasConceptScore W4387605344C175969161 @default.
- W4387605344 hasConceptScore W4387605344C178790620 @default.
- W4387605344 hasConceptScore W4387605344C185592680 @default.
- W4387605344 hasConceptScore W4387605344C201571599 @default.
- W4387605344 hasConceptScore W4387605344C2778252442 @default.
- W4387605344 hasConceptScore W4387605344C2779995066 @default.
- W4387605344 hasConceptScore W4387605344C2780301668 @default.
- W4387605344 hasConceptScore W4387605344C2781087480 @default.
- W4387605344 hasConceptScore W4387605344C41625074 @default.
- W4387605344 hasConceptScore W4387605344C519063684 @default.
- W4387605344 hasConceptScore W4387605344C54355233 @default.
- W4387605344 hasConceptScore W4387605344C62478195 @default.
- W4387605344 hasConceptScore W4387605344C86803240 @default.
- W4387605344 hasConceptScore W4387605344C95444343 @default.
- W4387605344 hasIssue "200" @default.
- W4387605344 hasLocation W43876053441 @default.
- W4387605344 hasOpenAccess W4387605344 @default.
- W4387605344 hasPrimaryLocation W43876053441 @default.
- W4387605344 hasRelatedWork W1604252474 @default.
- W4387605344 hasRelatedWork W1975302996 @default.
- W4387605344 hasRelatedWork W1977179204 @default.
- W4387605344 hasRelatedWork W2061686998 @default.
- W4387605344 hasRelatedWork W2079638667 @default.
- W4387605344 hasRelatedWork W2166605724 @default.
- W4387605344 hasRelatedWork W2352540589 @default.
- W4387605344 hasRelatedWork W2412854149 @default.
- W4387605344 hasRelatedWork W2461242556 @default.
- W4387605344 hasRelatedWork W2462356275 @default.
- W4387605344 isParatext "false" @default.
- W4387605344 isRetracted "false" @default.
- W4387605344 workType "article" @default.