Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387621058> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W4387621058 abstract "After birth, most newborn mouse cardiomyocytes (CM) undergo a final cycle of cell division in the absence of cytokinesis, which leads to binucleation. The majority of subsequent increases in cardiac mass are attributable to cardiomyocyte hypertrophy, as a negligible number of new CM are created after birth. Mice with a heart-specific deletion of Dicer, a crucial enzyme necessary for microRNA maturation, die by the fifth postnatal day in spite of having normal cardiac morphology and function at birth. Despite the significance of this phenomenon, little is known about the molecular/genetic foundation for the change from hyperplastic to hypertrophic-based myocardial expansion that occurs in the neonatal mouse heart. We hypothesize that a microRNA is required for the normal transition from fetal to adult heart during the neonatal period. Heart microRNA profiles (3 arrays/time point) were measured from RNA was isolated from E19, 1, 3, 5, 7, 10, and 35-day-old mouse hearts (9 hearts/time point pooled). Bioinformatic analysis identified microRNA-205 as a possible participant in the cardiac transitional program. We observed a transient 20-fold increase in miR205 expression between postnatal days 1 and 5, with levels returning to baseline by day 10. In-situ hybridization revealed miR-205 expression is restricted to the heart's epicardium.We next generated mice with a CM-specific deletion of miR205 using MHCcre targeting of a floxed-miR205 allele. Mice lacking miR-205 in their CM appear normal at birth. Elevated cell cycle markers (Ki67 & phospho-pH3) were detected at postnatal day-14 with 20% larger hearts at postnatal day 14, and adult hearts measuring up to 50% larger than controls. In contrast, a CM-specific miR205 over-expression mouse model resulted in more CM being present at both 5-day and 14-day post-birth. Interestingly, no difference in cell-cycle markers or heart size was observed. miR205 directly targets YAP within the evolutionarily conserved hippo pathway, which regulates organ size. YAP protein expression is significantly increased in hearts lacking miR205. Both of the miR205-heart models present with altered Hippo signaling kinetics after birth. We conclude that miR205 regulates postnatal heart size by directly modulating the Hippo pathway." @default.
- W4387621058 created "2023-10-14" @default.
- W4387621058 creator A5025090427 @default.
- W4387621058 creator A5077870086 @default.
- W4387621058 creator A5084288633 @default.
- W4387621058 creator A5093060532 @default.
- W4387621058 date "2023-08-04" @default.
- W4387621058 modified "2023-10-15" @default.
- W4387621058 title "Abstract P3037: MicroRNA-205 Is Required For Normal Cell Cycle And Cardiac Growth In The Neonatal Mouse Heart." @default.
- W4387621058 doi "https://doi.org/10.1161/res.133.suppl_1.p3037" @default.
- W4387621058 hasPublicationYear "2023" @default.
- W4387621058 type Work @default.
- W4387621058 citedByCount "0" @default.
- W4387621058 crossrefType "journal-article" @default.
- W4387621058 hasAuthorship W4387621058A5025090427 @default.
- W4387621058 hasAuthorship W4387621058A5077870086 @default.
- W4387621058 hasAuthorship W4387621058A5084288633 @default.
- W4387621058 hasAuthorship W4387621058A5093060532 @default.
- W4387621058 hasConcept C104317684 @default.
- W4387621058 hasConcept C105580179 @default.
- W4387621058 hasConcept C111566952 @default.
- W4387621058 hasConcept C126322002 @default.
- W4387621058 hasConcept C134018914 @default.
- W4387621058 hasConcept C145059251 @default.
- W4387621058 hasConcept C145103041 @default.
- W4387621058 hasConcept C1491633281 @default.
- W4387621058 hasConcept C16685009 @default.
- W4387621058 hasConcept C167414201 @default.
- W4387621058 hasConcept C172680121 @default.
- W4387621058 hasConcept C22615655 @default.
- W4387621058 hasConcept C2778198053 @default.
- W4387621058 hasConcept C2779234561 @default.
- W4387621058 hasConcept C2779867133 @default.
- W4387621058 hasConcept C29537977 @default.
- W4387621058 hasConcept C45472230 @default.
- W4387621058 hasConcept C54355233 @default.
- W4387621058 hasConcept C62425271 @default.
- W4387621058 hasConcept C67705224 @default.
- W4387621058 hasConcept C71924100 @default.
- W4387621058 hasConcept C81439078 @default.
- W4387621058 hasConcept C85813293 @default.
- W4387621058 hasConcept C86803240 @default.
- W4387621058 hasConceptScore W4387621058C104317684 @default.
- W4387621058 hasConceptScore W4387621058C105580179 @default.
- W4387621058 hasConceptScore W4387621058C111566952 @default.
- W4387621058 hasConceptScore W4387621058C126322002 @default.
- W4387621058 hasConceptScore W4387621058C134018914 @default.
- W4387621058 hasConceptScore W4387621058C145059251 @default.
- W4387621058 hasConceptScore W4387621058C145103041 @default.
- W4387621058 hasConceptScore W4387621058C1491633281 @default.
- W4387621058 hasConceptScore W4387621058C16685009 @default.
- W4387621058 hasConceptScore W4387621058C167414201 @default.
- W4387621058 hasConceptScore W4387621058C172680121 @default.
- W4387621058 hasConceptScore W4387621058C22615655 @default.
- W4387621058 hasConceptScore W4387621058C2778198053 @default.
- W4387621058 hasConceptScore W4387621058C2779234561 @default.
- W4387621058 hasConceptScore W4387621058C2779867133 @default.
- W4387621058 hasConceptScore W4387621058C29537977 @default.
- W4387621058 hasConceptScore W4387621058C45472230 @default.
- W4387621058 hasConceptScore W4387621058C54355233 @default.
- W4387621058 hasConceptScore W4387621058C62425271 @default.
- W4387621058 hasConceptScore W4387621058C67705224 @default.
- W4387621058 hasConceptScore W4387621058C71924100 @default.
- W4387621058 hasConceptScore W4387621058C81439078 @default.
- W4387621058 hasConceptScore W4387621058C85813293 @default.
- W4387621058 hasConceptScore W4387621058C86803240 @default.
- W4387621058 hasIssue "Suppl_1" @default.
- W4387621058 hasLocation W43876210581 @default.
- W4387621058 hasOpenAccess W4387621058 @default.
- W4387621058 hasPrimaryLocation W43876210581 @default.
- W4387621058 hasRelatedWork W1995296009 @default.
- W4387621058 hasRelatedWork W2084483378 @default.
- W4387621058 hasRelatedWork W2138708625 @default.
- W4387621058 hasRelatedWork W2350584147 @default.
- W4387621058 hasRelatedWork W2364838737 @default.
- W4387621058 hasRelatedWork W2372014155 @default.
- W4387621058 hasRelatedWork W3081475932 @default.
- W4387621058 hasRelatedWork W3158634669 @default.
- W4387621058 hasRelatedWork W4321610885 @default.
- W4387621058 hasRelatedWork W835149086 @default.
- W4387621058 hasVolume "133" @default.
- W4387621058 isParatext "false" @default.
- W4387621058 isRetracted "false" @default.
- W4387621058 workType "article" @default.