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• W4387640086 abstract "Apicomplexan haemoparasites generate significant morbidity and mortality in humans and other animals, particularly in many low-to-middle income countries. Malaria caused by Plasmodium remains responsible for some of the highest numbers of annual deaths of any human pathogen, whilst piroplasmids, such as Babesia and Theileria can have immense negative economic effects through livestock loss. Diagnosing haemoparasites via traditional methods like microscopy is challenging due to low-level and transient parasitaemia. PCR-based diagnostics overcome these limitations by being both highly sensitive and specific, but they may be unable to accurately detect coinfections or identify novel species. In contrast, next-generation sequencing (NGS)-based methods can characterize all pathogens from a group of interest concurrently, although, the short-read platforms previously used have been limited in the taxonomic resolution achievable. Here, we used Oxford Nanopore Technologies' (ONT) long-read MinION™ sequencer to conduct apicomplexan haemoparasite metabarcoding via sequencing the near full-length 18S ribosomal RNA gene, demonstrating its ability to detect Babesia, Hepatozoon, Neospora, Plasmodium, Theileria and Toxoplasma species. This method was tested on blood-extracted DNA from 100 dogs and the results benchmarked against qPCR and Illumina-based metabarcoding. For two common haemoparasites, nanopore sequencing performed as well as qPCR (kappa agreement statistics > 0.98), whilst also detecting one pathogen, Hepatozoon felis, missed by the other techniques. The long-reads obtained by nanopore sequencing provide an improved species-level taxonomic resolution whilst the method's broad applicability mean it can be used to explore apicomplexan communities from diverse mammalian hosts, on a portable sequencer that easily permits adaptation to field use." @default.
• W4387640086 created "2023-10-15" @default.
• W4387640086 creator A5033269751 @default.
• W4387640086 creator A5040055695 @default.
• W4387640086 creator A5042165812 @default.
• W4387640086 creator A5066584203 @default.
• W4387640086 date "2023-10-14" @default.
• W4387640086 modified "2023-10-16" @default.
• W4387640086 title "Metabarcoding using nanopore long‐read sequencing for the unbiased characterization of apicomplexan haemoparasites" @default.
• W4387640086 cites W1864123590 @default.
• W4387640086 cites W1920017664 @default.
• W4387640086 cites W1974265086 @default.
• W4387640086 cites W1975561097 @default.
• W4387640086 cites W1981981096 @default.
• W4387640086 cites W1983879622 @default.
• W4387640086 cites W1995173991 @default.
• W4387640086 cites W1997557207 @default.
• W4387640086 cites W2000230397 @default.
• W4387640086 cites W2003916212 @default.
• W4387640086 cites W2006001612 @default.
• W4387640086 cites W2030202766 @default.
• W4387640086 cites W2036897871 @default.
• W4387640086 cites W2038543829 @default.
• W4387640086 cites W2046342484 @default.
• W4387640086 cites W2055043387 @default.
• W4387640086 cites W2058404274 @default.
• W4387640086 cites W2059880410 @default.
• W4387640086 cites W2060284304 @default.
• W4387640086 cites W2060733353 @default.
• W4387640086 cites W2095554063 @default.
• W4387640086 cites W2099221601 @default.
• W4387640086 cites W2121546207 @default.
• W4387640086 cites W2129628333 @default.
• W4387640086 cites W2135218835 @default.
• W4387640086 cites W2163774862 @default.
• W4387640086 cites W2166171121 @default.
• W4387640086 cites W2401404581 @default.
• W4387640086 cites W2418163421 @default.
• W4387640086 cites W2516363611 @default.
• W4387640086 cites W2575750030 @default.
• W4387640086 cites W2589659933 @default.
• W4387640086 cites W2594269880 @default.
• W4387640086 cites W2601243251 @default.
• W4387640086 cites W2611957242 @default.
• W4387640086 cites W2735741826 @default.
• W4387640086 cites W2737928538 @default.
• W4387640086 cites W2766330461 @default.
• W4387640086 cites W2792623274 @default.
• W4387640086 cites W2801174474 @default.
• W4387640086 cites W2803457956 @default.
• W4387640086 cites W2866880918 @default.
• W4387640086 cites W2889326414 @default.
• W4387640086 cites W2896964253 @default.
• W4387640086 cites W2903632233 @default.
• W4387640086 cites W2909145450 @default.
• W4387640086 cites W2909919296 @default.
• W4387640086 cites W2918200658 @default.
• W4387640086 cites W2925255285 @default.
• W4387640086 cites W2945256932 @default.
• W4387640086 cites W2950354111 @default.
• W4387640086 cites W2950962479 @default.
• W4387640086 cites W2951254987 @default.
• W4387640086 cites W2951912016 @default.
• W4387640086 cites W2963276645 @default.
• W4387640086 cites W2965228978 @default.
• W4387640086 cites W2969305323 @default.
• W4387640086 cites W2972045911 @default.
• W4387640086 cites W2973113033 @default.
• W4387640086 cites W2995604505 @default.
• W4387640086 cites W2996923971 @default.
• W4387640086 cites W3010792071 @default.
• W4387640086 cites W3014244252 @default.
• W4387640086 cites W3014641335 @default.
• W4387640086 cites W3016764658 @default.
• W4387640086 cites W3035777835 @default.
• W4387640086 cites W3083512970 @default.
• W4387640086 cites W3093800398 @default.
• W4387640086 cites W3110189587 @default.
• W4387640086 cites W3112259453 @default.
• W4387640086 cites W3120666957 @default.
• W4387640086 cites W3134082414 @default.
• W4387640086 cites W3135959001 @default.
• W4387640086 cites W3161152079 @default.
• W4387640086 cites W3162248102 @default.
• W4387640086 cites W3167806572 @default.
• W4387640086 cites W3202060848 @default.
• W4387640086 cites W3213101281 @default.
• W4387640086 cites W4205138612 @default.
• W4387640086 cites W4220914200 @default.
• W4387640086 cites W4220944523 @default.
• W4387640086 cites W4232591880 @default.
• W4387640086 cites W4236406261 @default.
• W4387640086 cites W4283715818 @default.
• W4387640086 cites W4292680069 @default.
• W4387640086 cites W4294214983 @default.
• W4387640086 cites W4298087127 @default.
• W4387640086 cites W4306410006 @default.
• W4387640086 cites W4308347926 @default.
• W4387640086 cites W4311361671 @default.
• W4387640086 doi "https://doi.org/10.1111/1755-0998.13878" @default.

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