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- W4387645204 abstract "The study of myeloid-derived suppressor cells (MDSCs) has been commonly reported in the context of cancer immunology. MDSCs play a key role in cancer growth and progression by inhibiting both innate and adaptive immunity. In addition to the immunosuppressive function of MDSCs in cancer, a novel function of MDSCs as osteoclast precursors has recently been attracting attention. Because monocytic-MDSCs (M-MDSCs) are derived from the same myeloid lineage as macrophages, which are osteoclast progenitors, M-MDSCs can undergo differentiation into osteoclasts, contributing to bone destruction not only in the cancer microenvironment but also in inflammatory conditions including obesity and osteoarthritis. Herein, we present details of the technique to evaluate osteoclasts in vitro, as well as specific techniques to isolate M-MDSCs and identify them. This protocol can be easily adapted to isolate M-MDSCs from most pathologic conditions for easy evaluation." @default.
- W4387645204 created "2023-10-15" @default.
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- W4387645204 date "2023-01-01" @default.
- W4387645204 modified "2023-10-15" @default.
- W4387645204 title "In vitro osteoclastogenesis assessment using murine myeloid-derived suppressor cells" @default.
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- W4387645204 doi "https://doi.org/10.1016/bs.mcb.2023.04.007" @default.
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