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- W4387661736 abstract "RNA-DNA hybrids are epigenetic features of genomes that provide a diverse and growing range of activities. Understanding of these functions has been informed by characterising the proteins that interact with the hybrids, but all such analyses have so far focused on mammals, meaning it is unclear if a similar spectrum of RNA-DNA hybrid interactors is found in other eukaryotes. The African trypanosome is a single-cell eukaryotic parasite of the Discoba grouping and displays substantial divergence in several aspects of core biology from its mammalian host. Here, we show that DNA-RNA hybrid immunoprecipitation coupled with mass spectrometry recovers 602 putative interactors in T. brucei mammal- and insect-infective cells, some providing activities also found in mammals and some lineage-specific. We demonstrate that loss of three factors, two putative helicases and a RAD51 paralogue, alters T. brucei nuclear RNA-DNA hybrid and DNA damage levels. Moreover, loss of each factor affects the operation of the parasite immune survival mechanism of antigenic variation. Thus, our work reveals the broad range of activities contributed by RNA-DNA hybrids to T. brucei biology, including new functions in host immune evasion as well as activities likely fundamental to eukaryotic genome function." @default.
- W4387661736 created "2023-10-17" @default.
- W4387661736 creator A5026580693 @default.
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- W4387661736 creator A5068074966 @default.
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- W4387661736 creator A5091941555 @default.
- W4387661736 date "2023-10-16" @default.
- W4387661736 modified "2023-10-17" @default.
- W4387661736 title "Immunoprecipitation of RNA–DNA hybrid interacting proteins in <i>Trypanosoma brucei</i> reveals conserved and novel activities, including in the control of surface antigen expression needed for immune evasion by antigenic variation" @default.
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