Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387662904> ?p ?o ?g. }
- W4387662904 abstract "Abstract 5‐Formylcytosine (f 5 C) modification is present in human mitochondrial methionine tRNA (mt‐tRNA Met ) and cytosolic leucine tRNA (ct‐tRNA Leu ), with their formation mediated by NSUN3 and ALKBH1. f 5 C has also been detected in yeast mRNA and human tRNA, but its transcriptome‐wide distribution in mammals has not been studied. Here we report f 5 C‐seq, a quantitative sequencing method to map f 5 C transcriptome‐wide in HeLa and mouse embryonic stem cells (mESCs). We show that f 5 C in RNA can be reduced to dihydrouracil (DHU) by pic‐borane, and DHU can be exclusively read as T during reverse transcription (RT) reaction, allowing the detection and quantification of f 5 C sites by a unique C‐to‐T mutation signature. We validated f 5 C‐seq by identifying and quantifying the two known f 5 C sites in tRNA, in which the f 5 C modification fractions dropped significantly in ALKBH1‐depleted cells. By applying f 5 C‐seq to chromatin‐associated RNA (caRNA), we identified several highly modified f 5 C sites in HeLa and mouse embryonic stem cells (mESC)." @default.
- W4387662904 created "2023-10-17" @default.
- W4387662904 creator A5003648553 @default.
- W4387662904 creator A5003653959 @default.
- W4387662904 creator A5012805315 @default.
- W4387662904 creator A5020007405 @default.
- W4387662904 creator A5021439461 @default.
- W4387662904 creator A5025003914 @default.
- W4387662904 creator A5029667563 @default.
- W4387662904 creator A5048632663 @default.
- W4387662904 creator A5066588238 @default.
- W4387662904 creator A5091114864 @default.
- W4387662904 date "2023-10-16" @default.
- W4387662904 modified "2023-10-17" @default.
- W4387662904 title "A Quantitative Sequencing Method for 5‐Formylcytosine in RNA" @default.
- W4387662904 cites W1984066495 @default.
- W4387662904 cites W1989457196 @default.
- W4387662904 cites W1997453048 @default.
- W4387662904 cites W2021621793 @default.
- W4387662904 cites W2024912820 @default.
- W4387662904 cites W2027593734 @default.
- W4387662904 cites W2031388575 @default.
- W4387662904 cites W2031657400 @default.
- W4387662904 cites W2038449573 @default.
- W4387662904 cites W2038722690 @default.
- W4387662904 cites W2039179483 @default.
- W4387662904 cites W2043472013 @default.
- W4387662904 cites W2065847836 @default.
- W4387662904 cites W2114147818 @default.
- W4387662904 cites W2126165721 @default.
- W4387662904 cites W2132762486 @default.
- W4387662904 cites W2161890613 @default.
- W4387662904 cites W2175514210 @default.
- W4387662904 cites W2231633301 @default.
- W4387662904 cites W2252643587 @default.
- W4387662904 cites W2271549028 @default.
- W4387662904 cites W2271995006 @default.
- W4387662904 cites W2293389458 @default.
- W4387662904 cites W2358488663 @default.
- W4387662904 cites W2491926383 @default.
- W4387662904 cites W2520138650 @default.
- W4387662904 cites W2546967042 @default.
- W4387662904 cites W2579273737 @default.
- W4387662904 cites W2599498000 @default.
- W4387662904 cites W2606518593 @default.
- W4387662904 cites W2610162195 @default.
- W4387662904 cites W2753767186 @default.
- W4387662904 cites W2762798231 @default.
- W4387662904 cites W2764301500 @default.
- W4387662904 cites W2766140892 @default.
- W4387662904 cites W2766790922 @default.
- W4387662904 cites W2793859233 @default.
- W4387662904 cites W2901932654 @default.
- W4387662904 cites W2916127307 @default.
- W4387662904 cites W2940752764 @default.
- W4387662904 cites W2941132662 @default.
- W4387662904 cites W2949844417 @default.
- W4387662904 cites W2964438344 @default.
- W4387662904 cites W2971063457 @default.
- W4387662904 cites W2974694861 @default.
- W4387662904 cites W2999012956 @default.
- W4387662904 cites W3042640607 @default.
- W4387662904 cites W3217198278 @default.
- W4387662904 cites W4214534637 @default.
- W4387662904 cites W4220674994 @default.
- W4387662904 cites W4285799774 @default.
- W4387662904 cites W4306411156 @default.
- W4387662904 cites W4307495488 @default.
- W4387662904 cites W4307500724 @default.
- W4387662904 cites W4310058940 @default.
- W4387662904 cites W4313454095 @default.
- W4387662904 cites W4362459266 @default.
- W4387662904 doi "https://doi.org/10.1002/ijch.202300111" @default.
- W4387662904 hasPublicationYear "2023" @default.
- W4387662904 type Work @default.
- W4387662904 citedByCount "0" @default.
- W4387662904 crossrefType "journal-article" @default.
- W4387662904 hasAuthorship W4387662904A5003648553 @default.
- W4387662904 hasAuthorship W4387662904A5003653959 @default.
- W4387662904 hasAuthorship W4387662904A5012805315 @default.
- W4387662904 hasAuthorship W4387662904A5020007405 @default.
- W4387662904 hasAuthorship W4387662904A5021439461 @default.
- W4387662904 hasAuthorship W4387662904A5025003914 @default.
- W4387662904 hasAuthorship W4387662904A5029667563 @default.
- W4387662904 hasAuthorship W4387662904A5048632663 @default.
- W4387662904 hasAuthorship W4387662904A5066588238 @default.
- W4387662904 hasAuthorship W4387662904A5091114864 @default.
- W4387662904 hasBestOaLocation W43876629041 @default.
- W4387662904 hasConcept C104317684 @default.
- W4387662904 hasConcept C105580179 @default.
- W4387662904 hasConcept C132917006 @default.
- W4387662904 hasConcept C141231307 @default.
- W4387662904 hasConcept C145103041 @default.
- W4387662904 hasConcept C1491633281 @default.
- W4387662904 hasConcept C150194340 @default.
- W4387662904 hasConcept C153911025 @default.
- W4387662904 hasConcept C153957851 @default.
- W4387662904 hasConcept C162317418 @default.
- W4387662904 hasConcept C185592680 @default.
- W4387662904 hasConcept C2776449523 @default.