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- W4387665057 abstract "The Histidine-brace (His-brace) copper-binding site, composed of Cu(His)2 with a backbone amine, is found in metalloproteins with diverse functions. A primary example is lytic polysaccharide monooxygenase (LPMO), a class of enzymes that catalyze the oxidative depolymerization of polysaccharides, providing not only an energy source for native microorganisms but also a route to more effective industrial biomass conversion. Despite its importance, how the Cu His-brace site performs this unique and challenging oxidative depolymerization reaction remains to be understood. To answer this question, we have designed a biosynthetic model of LPMO by incorporating the Cu His-brace motif into azurin, an electron transfer protein. Spectroscopic studies, including ultraviolet-visible (UV-Vis) absorption and electron paramagnetic resonance, confirm copper binding at the designed His-brace site. Moreover, the designed protein is catalytically active towards both cellulose and starch, the native substrates of LPMO, generating degraded oligosaccharides with multiturnovers by C1 oxidation. It also performs oxidative cleavage of the model substrate 4-nitrophenyl-D-glucopyranoside, achieving a turnover number ~9% of that of a native LPMO assayed under identical conditions. This work presents a rationally designed artificial metalloenzyme that acts as a structural and functional mimic of LPMO, which provides a promising system for understanding the role of the Cu His-brace site in LPMO activity and potential application in polysaccharide degradation." @default.
- W4387665057 created "2023-10-17" @default.
- W4387665057 creator A5001269533 @default.
- W4387665057 creator A5024807731 @default.
- W4387665057 creator A5030686904 @default.
- W4387665057 creator A5038617650 @default.
- W4387665057 creator A5048778417 @default.
- W4387665057 date "2023-10-16" @default.
- W4387665057 modified "2023-10-18" @default.
- W4387665057 title "A designed Copper Histidine-brace enzyme for oxidative depolymerization of polysaccharides as a model of lytic polysaccharide monooxygenase" @default.
- W4387665057 cites W1535193939 @default.
- W4387665057 cites W1849447163 @default.
- W4387665057 cites W1967796434 @default.
- W4387665057 cites W1976621889 @default.
- W4387665057 cites W1978754214 @default.
- W4387665057 cites W1987460216 @default.
- W4387665057 cites W1993747047 @default.
- W4387665057 cites W1994887179 @default.
- W4387665057 cites W1995241084 @default.
- W4387665057 cites W1997124914 @default.
- W4387665057 cites W2002838322 @default.
- W4387665057 cites W2005800375 @default.
- W4387665057 cites W2005984466 @default.
- W4387665057 cites W2007329328 @default.
- W4387665057 cites W2016278529 @default.
- W4387665057 cites W2019555995 @default.
- W4387665057 cites W2023071558 @default.
- W4387665057 cites W2028291957 @default.
- W4387665057 cites W2034571753 @default.
- W4387665057 cites W2036189255 @default.
- W4387665057 cites W2044563572 @default.
- W4387665057 cites W2064013442 @default.
- W4387665057 cites W2073039007 @default.
- W4387665057 cites W2073110962 @default.
- W4387665057 cites W2077059894 @default.
- W4387665057 cites W2081653044 @default.
- W4387665057 cites W2087042268 @default.
- W4387665057 cites W2091605104 @default.
- W4387665057 cites W2104845210 @default.
- W4387665057 cites W2105005332 @default.
- W4387665057 cites W2106706785 @default.
- W4387665057 cites W2108274584 @default.
- W4387665057 cites W2110770347 @default.
- W4387665057 cites W2124540342 @default.
- W4387665057 cites W2126606211 @default.
- W4387665057 cites W2131888035 @default.
- W4387665057 cites W2132876121 @default.
- W4387665057 cites W2146051607 @default.
- W4387665057 cites W2179113683 @default.
- W4387665057 cites W2189335743 @default.
- W4387665057 cites W2281956162 @default.
- W4387665057 cites W2283136192 @default.
- W4387665057 cites W2291103326 @default.
- W4387665057 cites W2303145251 @default.
- W4387665057 cites W2313035794 @default.
- W4387665057 cites W2329280385 @default.
- W4387665057 cites W2344156231 @default.
- W4387665057 cites W2561236934 @default.
- W4387665057 cites W2568637369 @default.
- W4387665057 cites W2578282122 @default.
- W4387665057 cites W2727106131 @default.
- W4387665057 cites W2735839143 @default.
- W4387665057 cites W2746397541 @default.
- W4387665057 cites W2768059701 @default.
- W4387665057 cites W2770036214 @default.
- W4387665057 cites W2785236199 @default.
- W4387665057 cites W2785833536 @default.
- W4387665057 cites W2786484106 @default.
- W4387665057 cites W2800725631 @default.
- W4387665057 cites W2885883963 @default.
- W4387665057 cites W2896446106 @default.
- W4387665057 cites W2915890876 @default.
- W4387665057 cites W2923384196 @default.
- W4387665057 cites W2924793379 @default.
- W4387665057 cites W2941961750 @default.
- W4387665057 cites W2944342539 @default.
- W4387665057 cites W2949240035 @default.
- W4387665057 cites W2951802228 @default.
- W4387665057 cites W2969895547 @default.
- W4387665057 cites W2982477590 @default.
- W4387665057 cites W2985763446 @default.
- W4387665057 cites W2999365647 @default.
- W4387665057 cites W3000029748 @default.
- W4387665057 cites W3014901932 @default.
- W4387665057 cites W3025851418 @default.
- W4387665057 cites W3044421546 @default.
- W4387665057 cites W3046034062 @default.
- W4387665057 cites W3093427744 @default.
- W4387665057 cites W3126069294 @default.
- W4387665057 cites W3131614063 @default.
- W4387665057 cites W3135300440 @default.
- W4387665057 cites W3168436232 @default.
- W4387665057 cites W3177155684 @default.
- W4387665057 cites W3184425122 @default.
- W4387665057 cites W3189464306 @default.
- W4387665057 cites W3200932838 @default.
- W4387665057 cites W3209965060 @default.
- W4387665057 cites W4200074081 @default.
- W4387665057 cites W4212804863 @default.
- W4387665057 cites W4220793563 @default.