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• W4387673345 abstract "ABSTRACT Among the 16 two-component systems in the opportunistic human pathogen Staphylococcus aureus , only WalKR is essential. Like the orthologous systems in other Bacillota, S. aureus WalKR controls autolysins involved in peptidoglycan remodeling and is therefore intimately involved in cell division. However, despite the importance of WalKR in S. aureus , the basis for its essentiality is not understood and the regulon is poorly defined. Here, we defined a consensus WalR DNA-binding motif and the direct WalKR regulon by using functional genomics, including chromatin immunoprecipitation sequencing, with a panel of isogenic walKR mutants that had a spectrum of altered activities. Consistent with prior findings, the direct regulon includes multiple autolysin genes. However, this work also revealed that WalR directly regulates at least five essential genes involved in lipoteichoic acid synthesis ( ltaS ): translation ( rplK ), DNA compaction ( hup ), initiation of DNA replication ( dnaA , hup ) and purine nucleotide metabolism ( prs ). Thus, WalKR in S. aureus serves as a polyfunctional regulator that contributes to fundamental control over critical cell processes by coordinately linking cell wall homeostasis with purine biosynthesis, protein biosynthesis, and DNA replication. Our findings further address the essentiality of this locus and highlight the importance of WalKR as a bona fide target for novel anti-staphylococcal therapeutics. IMPORTANCE The opportunistic human pathogen Staphylococcus aureus uses an array of protein sensing systems called two-component systems (TCS) to sense environmental signals and adapt its physiology in response by regulating different genes. This sensory network is key to S. aureus versatility and success as a pathogen. Here, we reveal for the first time the full extent of the regulatory network of WalKR, the only staphylococcal TCS that is indispensable for survival under laboratory conditions. We found that WalKR is a master regulator of cell growth, coordinating the expression of genes from multiple, fundamental S. aureus cellular processes, including those involved in maintaining cell wall metabolism, protein biosynthesis, nucleotide metabolism, and the initiation of DNA replication." @default.
• W4387673345 created "2023-10-17" @default.
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• W4387673345 date "2023-10-16" @default.
• W4387673345 modified "2023-10-17" @default.
• W4387673345 title "The two-component system WalKR provides an essential link between cell wall homeostasis and DNA replication in <i>Staphylococcus aureus</i>" @default.
• W4387673345 cites W122179485 @default.
• W4387673345 cites W1507853369 @default.
• W4387673345 cites W1835426283 @default.
• W4387673345 cites W1980986357 @default.
• W4387673345 cites W1986618222 @default.
• W4387673345 cites W1993045940 @default.
• W4387673345 cites W2005518049 @default.
• W4387673345 cites W2033065712 @default.
• W4387673345 cites W2038558437 @default.
• W4387673345 cites W2044062947 @default.
• W4387673345 cites W2046700055 @default.
• W4387673345 cites W2054646594 @default.
• W4387673345 cites W2061514792 @default.
• W4387673345 cites W2062797919 @default.
• W4387673345 cites W2065894261 @default.
• W4387673345 cites W2077664655 @default.
• W4387673345 cites W2097294790 @default.
• W4387673345 cites W2103115554 @default.
• W4387673345 cites W2105719418 @default.
• W4387673345 cites W2106246537 @default.
• W4387673345 cites W2107277218 @default.
• W4387673345 cites W2109834591 @default.
• W4387673345 cites W2110055201 @default.
• W4387673345 cites W2114376976 @default.
• W4387673345 cites W2118291047 @default.
• W4387673345 cites W2123708388 @default.
• W4387673345 cites W2127113854 @default.
• W4387673345 cites W2130412867 @default.
• W4387673345 cites W2131953542 @default.
• W4387673345 cites W2134943393 @default.
• W4387673345 cites W2135102440 @default.
• W4387673345 cites W2137526110 @default.
• W4387673345 cites W2138701259 @default.
• W4387673345 cites W2139865900 @default.
• W4387673345 cites W2141676495 @default.
• W4387673345 cites W2143170981 @default.
• W4387673345 cites W2145443843 @default.
• W4387673345 cites W2148212192 @default.
• W4387673345 cites W2148968881 @default.
• W4387673345 cites W2150491437 @default.
• W4387673345 cites W2150927648 @default.
• W4387673345 cites W2151693231 @default.
• W4387673345 cites W2153453937 @default.
• W4387673345 cites W2155397502 @default.
• W4387673345 cites W2158266834 @default.
• W4387673345 cites W2165434041 @default.
• W4387673345 cites W2167160669 @default.
• W4387673345 cites W2169614539 @default.
• W4387673345 cites W2212307922 @default.
• W4387673345 cites W2304212006 @default.
• W4387673345 cites W2406118624 @default.
• W4387673345 cites W2433647462 @default.
• W4387673345 cites W2467628122 @default.
• W4387673345 cites W2508929473 @default.
• W4387673345 cites W2511476802 @default.
• W4387673345 cites W2547755118 @default.
• W4387673345 cites W2550570544 @default.
• W4387673345 cites W2558452363 @default.
• W4387673345 cites W2564992943 @default.
• W4387673345 cites W2605735428 @default.
• W4387673345 cites W2737279591 @default.
• W4387673345 cites W2764155647 @default.
• W4387673345 cites W2770806876 @default.
• W4387673345 cites W2788690034 @default.
• W4387673345 cites W2793875288 @default.
• W4387673345 cites W2795762451 @default.
• W4387673345 cites W2801364533 @default.
• W4387673345 cites W2888204577 @default.
• W4387673345 cites W2944479012 @default.
• W4387673345 cites W2949452547 @default.
• W4387673345 cites W2950378656 @default.
• W4387673345 cites W2957015183 @default.
• W4387673345 cites W2969267101 @default.
• W4387673345 cites W2971098387 @default.
• W4387673345 cites W2982028528 @default.
• W4387673345 cites W2983471436 @default.

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