FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387674399> ?p ?o ?g. }

• W4387674399 abstract "Abstract Murine Natural Killer cells were cultivated in vitro to isolate NK-derived exosomes. Subsequent quantification via qPCR confirmed enrichment of miR-1249-3p. Ana-1 murine macrophages were cultured in vitro and subsequently inoculated with Mycobacterium tuberculosis (MTB) strain H37Rv. NK-exo and NK-exo miR-1249-3p were separately applied to the infection model, followed by immunological assays conducted post-48-hour co-culture. Western blot analyses corroborated that NK-exo exhibited exosomal marker proteins Granzyme A (GzmA), Granzyme B (GzmB), and Perforin (PFN), alongside a notable enrichment of miR-1249-3p. Functionally, NK-exo augmented the expression levels of Caspase-9,-8, and − 3, as well as PARP, while attenuating the expression of NLRP3, ASC, and Cleaved-Caspase-1. Furthermore, qPCR demonstrated an up-regulation of Caspase-9, -8, and − 3, along with pro-apoptotic factors Bax and Bid, and a concomitant down-regulation of the anti-apoptotic factor Bcl-2. The expression levels of inflammatory markers ASC, NLRP3, Cleaved-Caspase-1, and IL-1β were concomitantly decreased. ELISA findings indicated diminished levels of TNF-α and ROS secretion. NK-exo miR-1249-3p specifically targeted and attenuated the expression of SKOR-1, engendering up-regulation of apoptosis-associated proteins and down-regulation of inflammation-related proteins, consequently affecting cellular fate.Our empirical evidence substantiates that NK-exo induces macrophage apoptosis, thereby mitigating MTB survival. Furthermore, NK-exo miR-1249-3p directly targets and inhibits SKOR-1 expression, leading to macrophage apoptosis and consequently hampering the proliferation of MTB. The data implicate the potential therapeutic relevance of NK-exo and miR-1249-3p in managing drug-resistant tuberculosis." @default.
• W4387674399 created "2023-10-17" @default.
• W4387674399 creator A5007279463 @default.
• W4387674399 creator A5007348590 @default.
• W4387674399 creator A5022244759 @default.
• W4387674399 creator A5027118370 @default.
• W4387674399 creator A5028828034 @default.
• W4387674399 creator A5032341066 @default.
• W4387674399 creator A5034397434 @default.
• W4387674399 creator A5043300709 @default.
• W4387674399 creator A5049513613 @default.
• W4387674399 creator A5069472515 @default.
• W4387674399 creator A5079916474 @default.
• W4387674399 creator A5091028074 @default.
• W4387674399 date "2023-10-16" @default.
• W4387674399 modified "2023-10-17" @default.
• W4387674399 title "NK-derived exosome miR-1249-3p inhibits Mycobacterium tuberculosis survival in macrophages by targeting SKOR1" @default.
• W4387674399 cites W2011089730 @default.
• W4387674399 cites W2025441450 @default.
• W4387674399 cites W2032735323 @default.
• W4387674399 cites W2035256770 @default.
• W4387674399 cites W2083911670 @default.
• W4387674399 cites W2592524105 @default.
• W4387674399 cites W2734802465 @default.
• W4387674399 cites W2789533682 @default.
• W4387674399 cites W2805148808 @default.
• W4387674399 cites W2808292810 @default.
• W4387674399 cites W2904070003 @default.
• W4387674399 cites W2919373065 @default.
• W4387674399 cites W2921260058 @default.
• W4387674399 cites W2962176767 @default.
• W4387674399 cites W2995593552 @default.
• W4387674399 cites W3005104290 @default.
• W4387674399 cites W3011689356 @default.
• W4387674399 cites W3015385912 @default.
• W4387674399 cites W3018017655 @default.
• W4387674399 cites W3036619015 @default.
• W4387674399 cites W3098223307 @default.
• W4387674399 cites W3149099499 @default.
• W4387674399 cites W3155131188 @default.
• W4387674399 cites W3182728948 @default.
• W4387674399 cites W3217738311 @default.
• W4387674399 cites W4294124092 @default.
• W4387674399 doi "https://doi.org/10.21203/rs.3.rs-3416640/v1" @default.
• W4387674399 hasPublicationYear "2023" @default.
• W4387674399 type Work @default.
• W4387674399 citedByCount "0" @default.
• W4387674399 crossrefType "posted-content" @default.
• W4387674399 hasAuthorship W4387674399A5007279463 @default.
• W4387674399 hasAuthorship W4387674399A5007348590 @default.
• W4387674399 hasAuthorship W4387674399A5022244759 @default.
• W4387674399 hasAuthorship W4387674399A5027118370 @default.
• W4387674399 hasAuthorship W4387674399A5028828034 @default.
• W4387674399 hasAuthorship W4387674399A5032341066 @default.
• W4387674399 hasAuthorship W4387674399A5034397434 @default.
• W4387674399 hasAuthorship W4387674399A5043300709 @default.
• W4387674399 hasAuthorship W4387674399A5049513613 @default.
• W4387674399 hasAuthorship W4387674399A5069472515 @default.
• W4387674399 hasAuthorship W4387674399A5079916474 @default.
• W4387674399 hasAuthorship W4387674399A5091028074 @default.
• W4387674399 hasBestOaLocation W43876743991 @default.
• W4387674399 hasConcept C104317684 @default.
• W4387674399 hasConcept C145059251 @default.
• W4387674399 hasConcept C153911025 @default.
• W4387674399 hasConcept C154317977 @default.
• W4387674399 hasConcept C183193105 @default.
• W4387674399 hasConcept C190283241 @default.
• W4387674399 hasConcept C202751555 @default.
• W4387674399 hasConcept C203014093 @default.
• W4387674399 hasConcept C20518536 @default.
• W4387674399 hasConcept C2776062744 @default.
• W4387674399 hasConcept C2776090121 @default.
• W4387674399 hasConcept C2776415932 @default.
• W4387674399 hasConcept C2779968557 @default.
• W4387674399 hasConcept C2780380082 @default.
• W4387674399 hasConcept C2781261824 @default.
• W4387674399 hasConcept C502942594 @default.
• W4387674399 hasConcept C55493867 @default.
• W4387674399 hasConcept C86803240 @default.
• W4387674399 hasConcept C8891405 @default.
• W4387674399 hasConcept C95444343 @default.
• W4387674399 hasConceptScore W4387674399C104317684 @default.
• W4387674399 hasConceptScore W4387674399C145059251 @default.
• W4387674399 hasConceptScore W4387674399C153911025 @default.
• W4387674399 hasConceptScore W4387674399C154317977 @default.
• W4387674399 hasConceptScore W4387674399C183193105 @default.
• W4387674399 hasConceptScore W4387674399C190283241 @default.
• W4387674399 hasConceptScore W4387674399C202751555 @default.
• W4387674399 hasConceptScore W4387674399C203014093 @default.
• W4387674399 hasConceptScore W4387674399C20518536 @default.
• W4387674399 hasConceptScore W4387674399C2776062744 @default.
• W4387674399 hasConceptScore W4387674399C2776090121 @default.
• W4387674399 hasConceptScore W4387674399C2776415932 @default.
• W4387674399 hasConceptScore W4387674399C2779968557 @default.
• W4387674399 hasConceptScore W4387674399C2780380082 @default.
• W4387674399 hasConceptScore W4387674399C2781261824 @default.
• W4387674399 hasConceptScore W4387674399C502942594 @default.
• W4387674399 hasConceptScore W4387674399C55493867 @default.
• W4387674399 hasConceptScore W4387674399C86803240 @default.
• W4387674399 hasConceptScore W4387674399C8891405 @default.

• next