FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387675503> ?p ?o ?g. }

• W4387675503 endingPage "125316" @default.
• W4387675503 startingPage "125316" @default.
• W4387675503 abstract "Ensuring effective monitoring of methotrexate (MTX) levels in the bloodstream of cancer patients undergoing high-dose methotrexate chemotherapy is crucial to prevent potentially harmful side effects. However, the absence of portable analytical devices suitable for point-of-care bedside monitoring has presented a significant obstacle to achieving real-time MTX monitoring. In this study, we developed an impedimetric immunosensor that doesn't require reagents for measuring MTX levels in undiluted human blood serum. This reagentless approach simplifies the assay process, enabling rapid and straightforward MTX quantification. The immunosensor transducer was fabricated by electrodepositing conductive network of porous multiwalled carbon nanotube@polypyrrole/polytyramine on screen-printed gold microchip electrode (SP–Au/MWCNT70@PPy-PTA). Polyclonal anti-MTX antibodies were immobilized on the film, acting as the immunorecognition element. Non-specific binding was prevented by blocking the transducer interface with denatured bovine serum albumin (dBSA) fibrils, resulting in SP-Au/MWCNT70@PPy-PTA/anti-MTXAb|dBSA film electrode. When MTX binds to the SP-Au/MWCNT70@PPy-PTA/anti-MTXAb|dBSA interface, the film conductance and electron transfer resistance changes. This conductivity attenuation allows for electrochemical impedimetric signal transduction without a redox-probe solution. The electrochemical impedance spectroscopy (EIS) results showed increased charge transfer resistance and phase angle as MTX concentrations increased. The SP-Au/MWCNT70@PPy-PTA/anti-MTXAb|dBSA demonstrated high sensitivity, with a linear response from 0.02 to 20.0 μM and a detection limit of 1.93 nM. The detection limit was 50 times lower than the intended safe level of MTX in human serum. The immunosensor exhibited minimal cross-reactivity with endogenous MTX analogs and serum proteins. The SP-Au/MWCNT70@PPy-PTA/anti-MTXAb|dBSA immunosensor presents a simple and rapid method for therapeutic drug monitoring compared to traditional immunoassay systems." @default.
• W4387675503 created "2023-10-17" @default.
• W4387675503 creator A5011752275 @default.
• W4387675503 creator A5014007181 @default.
• W4387675503 creator A5018008694 @default.
• W4387675503 creator A5053543825 @default.
• W4387675503 creator A5075485377 @default.
• W4387675503 creator A5077431868 @default.
• W4387675503 date "2023-10-01" @default.
• W4387675503 modified "2023-10-18" @default.
• W4387675503 title "Reagentless impedimetric immunosensor for monitoring of methotrexate in human blood serum using multiwalled carbon nanotube@polypyrrole/polytyramine film electrode" @default.
• W4387675503 cites W1980663618 @default.
• W4387675503 cites W1995599455 @default.
• W4387675503 cites W2020981611 @default.
• W4387675503 cites W2030785134 @default.
• W4387675503 cites W2038376244 @default.
• W4387675503 cites W2053723948 @default.
• W4387675503 cites W2056312674 @default.
• W4387675503 cites W2064967234 @default.
• W4387675503 cites W2073765087 @default.
• W4387675503 cites W2074387896 @default.
• W4387675503 cites W2077440173 @default.
• W4387675503 cites W2095421113 @default.
• W4387675503 cites W2142616277 @default.
• W4387675503 cites W2285586237 @default.
• W4387675503 cites W2315280303 @default.
• W4387675503 cites W2492743894 @default.
• W4387675503 cites W2747274212 @default.
• W4387675503 cites W2781741646 @default.
• W4387675503 cites W2890347511 @default.
• W4387675503 cites W2891740088 @default.
• W4387675503 cites W2914508449 @default.
• W4387675503 cites W2915035401 @default.
• W4387675503 cites W2949724286 @default.
• W4387675503 cites W2979313800 @default.
• W4387675503 cites W2983445155 @default.
• W4387675503 cites W2986077039 @default.
• W4387675503 cites W2995975509 @default.
• W4387675503 cites W3005664296 @default.
• W4387675503 cites W3005944646 @default.
• W4387675503 cites W3011233272 @default.
• W4387675503 cites W3020469634 @default.
• W4387675503 cites W3131491068 @default.
• W4387675503 cites W3167914420 @default.
• W4387675503 cites W3177728619 @default.
• W4387675503 cites W3191300645 @default.
• W4387675503 cites W3196319782 @default.
• W4387675503 cites W3201104558 @default.
• W4387675503 cites W3202661170 @default.
• W4387675503 cites W3206905988 @default.
• W4387675503 cites W4221137500 @default.
• W4387675503 cites W4285729826 @default.
• W4387675503 cites W4294098992 @default.
• W4387675503 cites W4308901208 @default.
• W4387675503 cites W4310859435 @default.
• W4387675503 cites W4372194772 @default.
• W4387675503 cites W4382583000 @default.
• W4387675503 doi "https://doi.org/10.1016/j.talanta.2023.125316" @default.
• W4387675503 hasPublicationYear "2023" @default.
• W4387675503 type Work @default.
• W4387675503 citedByCount "0" @default.
• W4387675503 crossrefType "journal-article" @default.
• W4387675503 hasAuthorship W4387675503A5011752275 @default.
• W4387675503 hasAuthorship W4387675503A5014007181 @default.
• W4387675503 hasAuthorship W4387675503A5018008694 @default.
• W4387675503 hasAuthorship W4387675503A5053543825 @default.
• W4387675503 hasAuthorship W4387675503A5075485377 @default.
• W4387675503 hasAuthorship W4387675503A5077431868 @default.
• W4387675503 hasConcept C119128265 @default.
• W4387675503 hasConcept C147789679 @default.
• W4387675503 hasConcept C160756335 @default.
• W4387675503 hasConcept C171250308 @default.
• W4387675503 hasConcept C17525397 @default.
• W4387675503 hasConcept C185592680 @default.
• W4387675503 hasConcept C192562407 @default.
• W4387675503 hasConcept C2777232022 @default.
• W4387675503 hasConcept C43617362 @default.
• W4387675503 hasConcept C513720949 @default.
• W4387675503 hasConcept C52859227 @default.
• W4387675503 hasConcept C55493867 @default.
• W4387675503 hasConcept C7040849 @default.
• W4387675503 hasConceptScore W4387675503C119128265 @default.
• W4387675503 hasConceptScore W4387675503C147789679 @default.
• W4387675503 hasConceptScore W4387675503C160756335 @default.
• W4387675503 hasConceptScore W4387675503C171250308 @default.
• W4387675503 hasConceptScore W4387675503C17525397 @default.
• W4387675503 hasConceptScore W4387675503C185592680 @default.
• W4387675503 hasConceptScore W4387675503C192562407 @default.
• W4387675503 hasConceptScore W4387675503C2777232022 @default.
• W4387675503 hasConceptScore W4387675503C43617362 @default.
• W4387675503 hasConceptScore W4387675503C513720949 @default.
• W4387675503 hasConceptScore W4387675503C52859227 @default.
• W4387675503 hasConceptScore W4387675503C55493867 @default.
• W4387675503 hasConceptScore W4387675503C7040849 @default.
• W4387675503 hasLocation W43876755031 @default.
• W4387675503 hasOpenAccess W4387675503 @default.
• W4387675503 hasPrimaryLocation W43876755031 @default.
• W4387675503 hasRelatedWork W1936924293 @default.

• next