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- W439001449 abstract "An important factor complicating the recovery of recombinant proteins from Escherichiacoli is their intracellular location. An alternative to the commonly used method of releasing these proteins by mechanical disruption is to chemically permeabilize the cells. The objective of this research was to characterize the protein release kinetics and mechanism of a permeabilization process using guanidine-HCl and Triton-X100. The protein release kinetics were determined as a function of the guanidine, Triton, and cell concentrations. Some of the advantages over mechanical disruption include avoidance of extensive fragmentation of the cells and retention of the nucleic acids inside the cell structure." @default.
- W439001449 created "2016-06-24" @default.
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- W439001449 date "1986-07-11" @default.
- W439001449 modified "2023-09-23" @default.
- W439001449 title "Separation, Recovery, and Purification in Biotechnology" @default.
- W439001449 doi "https://doi.org/10.1021/bk-1986-0314" @default.
- W439001449 hasPublicationYear "1986" @default.
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