FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W43902851> ?p ?o ?g. }

• W43902851 endingPage "6" @default.
• W43902851 startingPage "961" @default.
• W43902851 abstract "On the basis of sequence similarity, the crustean hyperglycemic hormone (CHH) family peptides have been classified into two types of hormones: type I and type II. Molt-inhibiting hormone (MIH) is a neuropeptide member of type II CHH family. Molting in shrimp is controlled by MIH and ecdysone. By inhibiting the synthesis of ecdysone in the Y-organ, MIH indirectly suppresses the molting activity of shrimp. In this study, we reported the cloning and characterization of 3 gene fragments encoding type II CHH family neuropeptides of the shrimp Fennropenaeus chinensis. According to the complementary DNA sequence of the mult-inhibiting hormone of Fennropenaeus chinensis, 3 primers were designed and synthesized. MP1 and MP2 are sense primers, and MP3 is anti-sense primer. Polymerase chain reaction was performed using genomic DNA of Fennropenaeus chinensis as template. Three PCR products were obtained using primers MP1 and MP3. Their sizes are about 600 bp, 850 bp, 1050 bp, respectively. A 580 bp PCR product was obtained using primers MP2 and MP3. All the 4 PCR products were cloned into pMD18-T vector. The recombinant clones were sequenced using ABI 310 Genetic Analyzer. After sequencing, all the DNA sequences were searched in the GenBank by Blast program to find similar gene sequences. The searching results revealed 3 DNA fragment sequences were of high similarity with CHH family neuropeptide genes from various crustean species. The 3 DNA fragments were named as NP1, NP2, and NP3. Their sizes were 540 bp, 601 bp, and 826 bp, respectively. Using the mRNA sequences with the most similarity to the 3 sequence fragments as reference, the gene structure of the 3 DNA fragment sequences was analyzed. The exons of 3 sequence fragments were aligned with their similar sequences by Clustal W program. Both NP1 and NP2 consisted of 1 intron and 2 exons. NP3 consisted of 2 introns and 3 exons. Sequence analysis suggested that these 3 products belonged to sequence fragments of neuropeptide gene of type II crustacean hyperglycemic hormone family. The exons of NP1, NP2, and NP3 had highest similarity respectively with mRNA of Pem-SCP-C2 and Pem-SGP-C1 both from Penaeus monodon, and FenchMIH from Fennropenaeus chinensis. The identities were 91.5%, 92.8%, 88.9%, respectively. The results suggest NP3 is a fragment of molt-inhibiting hormone gene of Fennropenaeus chinensis NP1 and NP2 are two fragments of neuropeptide genes of type II crustacean hyperglycemic hormone family, which were found in Fennropenaeus chinensis for the first time." @default.
• W43902851 created "2016-06-24" @default.
• W43902851 creator A5018099072 @default.
• W43902851 creator A5069020332 @default.
• W43902851 date "2003-10-01" @default.
• W43902851 modified "2023-09-23" @default.
• W43902851 title "[Cloning and analysis of three genes encoding type II CHH family neuropeptides from Fennropenaeus chinensis]." @default.
• W43902851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14669514" @default.
• W43902851 hasPublicationYear "2003" @default.
• W43902851 type Work @default.
• W43902851 sameAs 43902851 @default.
• W43902851 citedByCount "2" @default.
• W43902851 countsByYear W439028512015 @default.
• W43902851 countsByYear W439028512016 @default.
• W43902851 crossrefType "journal-article" @default.
• W43902851 hasAuthorship W43902851A5018099072 @default.
• W43902851 hasAuthorship W43902851A5069020332 @default.
• W43902851 hasConcept C104317684 @default.
• W43902851 hasConcept C121050878 @default.
• W43902851 hasConcept C141231307 @default.
• W43902851 hasConcept C153911025 @default.
• W43902851 hasConcept C178790620 @default.
• W43902851 hasConcept C185592680 @default.
• W43902851 hasConcept C187882448 @default.
• W43902851 hasConcept C19924922 @default.
• W43902851 hasConcept C199360897 @default.
• W43902851 hasConcept C2777563447 @default.
• W43902851 hasConcept C41008148 @default.
• W43902851 hasConcept C49105822 @default.
• W43902851 hasConcept C54355233 @default.
• W43902851 hasConcept C5911399 @default.
• W43902851 hasConcept C61053724 @default.
• W43902851 hasConcept C79029880 @default.
• W43902851 hasConcept C86803240 @default.
• W43902851 hasConceptScore W43902851C104317684 @default.
• W43902851 hasConceptScore W43902851C121050878 @default.
• W43902851 hasConceptScore W43902851C141231307 @default.
• W43902851 hasConceptScore W43902851C153911025 @default.
• W43902851 hasConceptScore W43902851C178790620 @default.
• W43902851 hasConceptScore W43902851C185592680 @default.
• W43902851 hasConceptScore W43902851C187882448 @default.
• W43902851 hasConceptScore W43902851C19924922 @default.
• W43902851 hasConceptScore W43902851C199360897 @default.
• W43902851 hasConceptScore W43902851C2777563447 @default.
• W43902851 hasConceptScore W43902851C41008148 @default.
• W43902851 hasConceptScore W43902851C49105822 @default.
• W43902851 hasConceptScore W43902851C54355233 @default.
• W43902851 hasConceptScore W43902851C5911399 @default.
• W43902851 hasConceptScore W43902851C61053724 @default.
• W43902851 hasConceptScore W43902851C79029880 @default.
• W43902851 hasConceptScore W43902851C86803240 @default.
• W43902851 hasIssue "10" @default.
• W43902851 hasLocation W439028511 @default.
• W43902851 hasOpenAccess W43902851 @default.
• W43902851 hasPrimaryLocation W439028511 @default.
• W43902851 hasRelatedWork W2168905894 @default.
• W43902851 hasRelatedWork W2350932564 @default.
• W43902851 hasRelatedWork W2359431220 @default.
• W43902851 hasRelatedWork W2361796239 @default.
• W43902851 hasRelatedWork W2364221357 @default.
• W43902851 hasRelatedWork W2367078647 @default.
• W43902851 hasRelatedWork W2367416754 @default.
• W43902851 hasRelatedWork W2370709181 @default.
• W43902851 hasRelatedWork W2375714649 @default.
• W43902851 hasRelatedWork W238985201 @default.
• W43902851 hasVolume "30" @default.
• W43902851 isParatext "false" @default.
• W43902851 isRetracted "false" @default.
• W43902851 magId "43902851" @default.
• W43902851 workType "article" @default.