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- W44126689 abstract "It has been demonstrated that Cd{sup 2+} accumulates in vacuoles of tobacco leaves exposed to 20 {mu}M Cd{sup 2+}, after 4 days of growth in Hoaglands medium. The accumulation of Cd{sup 2+} is also associated with the accumulation of Cd{sup 2+}-peptide (phytocelatin) in the vacuole. The transport of Cd{sup 2+} and/or Cd{sup 2+}-peptide across the tonoplast membrane may be energized by the H{sup +} electrochemical gradient that exists across this membrane and which is generated by an H{sup +}-pumping ATPase. In vitro 2 {mu}M Cd{sup 2+} inhibits oat root tonoplast H{sup +}-pumping ATPase by 50% with a Ki of approximately 4.0 {mu}M. However, exposure to 2- {mu}M Cd{sup 2+} for 4 days during germination and growth of oat seedlings causes a 100% increase in tonoplast H{sup +}-pumping ATPase activity. This increase on exposure to Cd{sup 2+} during growth may represent part of the physiological mechanism whereby plants accumulate Cd{sup 2+} and/or Cd{sup 2+}-peptide within the vacuole. This could also represent an acclimation of H{sup +}-pumping activity to Cd{sup 2+}. Current studies are testing the effects of Cd{sup 2+}-peptide, organic acids and other ligands on H{sup +}-pumping activity and Cd{sup 2+} transport in root derived tonoplast vesicles of oat and tobacco." @default.
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- W44126689 date "1991-05-01" @default.
- W44126689 modified "2023-09-27" @default.
- W44126689 title "Response of tonoplast H sup + -pump and ATPase to cadmium" @default.
- W44126689 hasPublicationYear "1991" @default.
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