Matches in SemOpenAlex for { <https://semopenalex.org/work/W44276427> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W44276427 endingPage "2618" @default.
- W44276427 startingPage "2606" @default.
- W44276427 abstract "A 180-kilodalton (kDa) protein (p180) was identified among the antigens for a panel of monoclonal antibodies raised against human fibroblast cell surface proteins. Binding studies with 125I-Fab' fragments of an anti-p180 monoclonal antibody demonstrated that 10 to 30% of p180 was located on the plasma membrane and that the remaining 70 to 90% was on intracellular membranes. p180 was rapidly internalized from the cell surface at 37 degrees C, and kinetic analyses indicated that this was a constitutive process followed by the recycling of p180 back to the plasma membrane. Morphological studies demonstrated that on the cell surface p180 was concentrated in coated pits, whereas inside the cell it was found in endosomes as suggested by its colocalization with the transferrin receptor. Immunoblot analysis with a polyclonal antiserum raised against purified human protein showed that p180 has a restricted distribution with expression at high levels in fibroblast cultures and in tissues containing cells of mesodermal origin. A biochemical characterization of p180 showed it to be a transmembrane glycoprotein with an extracellular domain, which consists of approximately 30 kDa of complex oligosaccharides attached to at least 45 kDa of the protein core. The cytoplasmic domain of p180 was found to contain a serine residue(s) that was phosphorylated both in vivo and in vitro by activated protein kinase C. p180 was purified by subjecting solubilized membrane proteins from a human osteosarcoma cell line to immunoaffinity chromatography and gel filtration. The N-terminal sequence information obtained from the purified protein showed no homology to other known proteins. It was concluded that p180 may be a novel recycling receptor which is highly restricted in its expression to fibroblastlike cells." @default.
- W44276427 created "2016-06-24" @default.
- W44276427 creator A5010612497 @default.
- W44276427 creator A5082269354 @default.
- W44276427 date "1990-06-01" @default.
- W44276427 modified "2023-10-18" @default.
- W44276427 title "p180, a novel recycling transmembrane glycoprotein with restricted cell type expression" @default.
- W44276427 cites W125261686 @default.
- W44276427 cites W1491181982 @default.
- W44276427 cites W1497356015 @default.
- W44276427 cites W1533042261 @default.
- W44276427 cites W1546031868 @default.
- W44276427 cites W1555650590 @default.
- W44276427 cites W1616609814 @default.
- W44276427 cites W1860351953 @default.
- W44276427 cites W1972711906 @default.
- W44276427 cites W1980939967 @default.
- W44276427 cites W1984351623 @default.
- W44276427 cites W1985672357 @default.
- W44276427 cites W1986316392 @default.
- W44276427 cites W1988681657 @default.
- W44276427 cites W2011147958 @default.
- W44276427 cites W2027076158 @default.
- W44276427 cites W2029991413 @default.
- W44276427 cites W2033785962 @default.
- W44276427 cites W2036229899 @default.
- W44276427 cites W2043999925 @default.
- W44276427 cites W2044140136 @default.
- W44276427 cites W2048375895 @default.
- W44276427 cites W2054385006 @default.
- W44276427 cites W2061012375 @default.
- W44276427 cites W2080580789 @default.
- W44276427 cites W2123513319 @default.
- W44276427 cites W2138466097 @default.
- W44276427 cites W2153151822 @default.
- W44276427 cites W2153823739 @default.
- W44276427 cites W2160845560 @default.
- W44276427 cites W2251380018 @default.
- W44276427 cites W4231565477 @default.
- W44276427 doi "https://doi.org/10.1128/mcb.10.6.2606-2618.1990" @default.
- W44276427 hasPublicationYear "1990" @default.
- W44276427 type Work @default.
- W44276427 sameAs 44276427 @default.
- W44276427 citedByCount "2" @default.
- W44276427 countsByYear W442764272022 @default.
- W44276427 crossrefType "journal-article" @default.
- W44276427 hasAuthorship W44276427A5010612497 @default.
- W44276427 hasAuthorship W44276427A5082269354 @default.
- W44276427 hasConcept C153911025 @default.
- W44276427 hasConcept C159654299 @default.
- W44276427 hasConcept C202751555 @default.
- W44276427 hasConcept C203014093 @default.
- W44276427 hasConcept C2780381497 @default.
- W44276427 hasConcept C55493867 @default.
- W44276427 hasConcept C86803240 @default.
- W44276427 hasConcept C956191 @default.
- W44276427 hasConceptScore W44276427C153911025 @default.
- W44276427 hasConceptScore W44276427C159654299 @default.
- W44276427 hasConceptScore W44276427C202751555 @default.
- W44276427 hasConceptScore W44276427C203014093 @default.
- W44276427 hasConceptScore W44276427C2780381497 @default.
- W44276427 hasConceptScore W44276427C55493867 @default.
- W44276427 hasConceptScore W44276427C86803240 @default.
- W44276427 hasConceptScore W44276427C956191 @default.
- W44276427 hasIssue "6" @default.
- W44276427 hasLocation W442764271 @default.
- W44276427 hasOpenAccess W44276427 @default.
- W44276427 hasPrimaryLocation W442764271 @default.
- W44276427 hasRelatedWork W2350566209 @default.
- W44276427 hasRelatedWork W2360867740 @default.
- W44276427 hasRelatedWork W2371689295 @default.
- W44276427 hasRelatedWork W2373874986 @default.
- W44276427 hasRelatedWork W2382768569 @default.
- W44276427 hasRelatedWork W2404970265 @default.
- W44276427 hasRelatedWork W2995425410 @default.
- W44276427 hasRelatedWork W3026200651 @default.
- W44276427 hasRelatedWork W3028649718 @default.
- W44276427 hasRelatedWork W3146540107 @default.
- W44276427 hasVolume "10" @default.
- W44276427 isParatext "false" @default.
- W44276427 isRetracted "false" @default.
- W44276427 magId "44276427" @default.
- W44276427 workType "article" @default.