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• W44386317 abstract "A genomic library of Pseudomonas syringae pv. phaseolicola race 3 was constructed in the cosmid vector pLAFR1 and maintained in Escherichia coli. Clones were transferred individually by conjugation into an isolate of race 1 of P.s. pv phaseolicola. Transconjugants were screened for alterations to the virulence phenotype of race 1 by inoculation into pods of Phaseolus vulgaris of the differential cvs. Canadian Wonder, Red Mexican and Tendergreen. None of the 842 clones tested altered the race 1 phenotype from avirulence to virulence on cv. Red Mexican, but two clones (p3A1000 and p3A2000), with a common 19·2-kb EcoR1 DNA fragment, altered the race 1 phenotype from virulence to avirulence on cv. Tendergreen. Leaves and pods of cv. Tendergreen, inoculated with race 1 (P3A1000) or race 1 (p3A2000), underwent a hypersensitive reaction (HR) identical to that caused by race 3. The virulence and avirulence, respectively of race 1 to cvs. Canadian Wonder and Red Mexican, was not affected by the presence of the cosmids. Race 1 (p3A1000) caused HR in leaves and pods of a range of bean genotypes known to be resistant to race 3 but susceptible to race 1. Race 1 (p3A1000) induced the same phytoalexin response and multiplied to the same limited extent as race 3 in pods of cv. Tendergreen. The clone p3A1000 conferred the avirulence phenotype of race 3 when transferred to race 2 of P.s. pv. phaseolicola but it did not affect the virulence of two isolates of P.S. pv. syringae or Xanthomonas campestris pv. phaseoli to the P. vulgaris cvs. tested." @default.
• W44386317 created "2016-06-24" @default.
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• W44386317 date "1989-04-01" @default.
• W44386317 modified "2023-10-13" @default.
• W44386317 title "Determinant of cultivar specific avirulence cloned from Pseudomonas syringae pv. phaseolicola race 3" @default.
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• W44386317 doi "https://doi.org/10.1016/0885-5765(89)90028-3" @default.
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