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- W44561147 abstract "Electroporation-mediated transformation (EMT) is widely used for gene delivery in many fungi. However, no such method has been developed for newly discovered fungus Piriformospora indica. Here, we describe an efficient protocol EMT using fungal mycelia and chlamydospores. Fresh grown mycelial fragments and germinated spores were transformed by electroporation. Transgenic colonies obtained were subjected to two rounds of selection on medium containing higher levels of the selective agent to eliminate false-positive colonies. Use of mycelia offers flexible experiment planning and a convenient source of experimental material for fungus that does not produce enough spores. Additionally, we also report the use of RNAi construct as a reporter in P. indica for functional expression. Using this protocol, transgenic fungus can be obtained in 3–6 weeks. This method offers new prospects for the genetic manipulations of this axenically cultivable, plant probiotic fungus." @default.
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- W44561147 date "2013-01-01" @default.
- W44561147 modified "2023-09-25" @default.
- W44561147 title "“Electrotransformation” Transformation System for Root Endophytic Fungus Piriformospora indica" @default.
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- W44561147 doi "https://doi.org/10.1007/978-3-642-33802-1_19" @default.
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