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- W44591738 abstract "Several explants from Fl hybrid seeds andaseptically-germinated seedlings of muskmelon Cucumismelo var. Birdie, were tested for their capacity forplant regeneration through direct organogenesis invitro.Excised cotyledons and - embryos from imbibed,ungerminated seeds were cultured on Murashige and Skoog(MS) medium containing 1.0 - 5.0 uM and 1.0-50.0 uM benzylaminopurine (BAP), kinetin, or isopentenyl adenine(2iP) respectively, in the presence or absence of 5.0 uMgibberellic acid (GA₃). Embryo explants producedcallus on all media tested, and only 39 of cotyledonexplants produced buds on medium containing 2.0 - 3.0 uMBAP.Unimbibed, testaless seeds were germinatedaseptically on MS medium in the presence of 1.0 - 50.0uM BAP, kinetin or 2 ip. On medium containing 3.0 uMBAP, 68% of cultures produced small buds in the regionof the cotyledonary node within three weeks. Theaddition of GAl (5.0 uM) or naphthalene acetic acid(NAA) (0.1 - 1.5 uM) did not increase the percentage ofcultures which produced buds.Cotyledonary nodes were subcultured to mediacontaining the same (3.0 UK) or lower concentrations ofBAP for two weeks and then to MS basal medium foranother two weeks to allow further bud development andshoot elongation. The highest number of shoots percotyl edonary node (9.7) was obtained when the firstsubculture medium contained 0.5 uM BAP.Individual shoots less than 1.0 cm, 1.0 - 2.5 cmand more than 2.5 cm in length, were tested for rooting ability in the presence of 3.0 uM IBA. The highestpercentage rooting (49) occurred in shoots more than1.0cm long. Further studies on root induction showedthat the highest percentage of rooting (85% ), occurredwhen shoots 1.0 - 2.5 cm lonq were cultured in thelight, on filter-paper bridqes in liquid, half-strengthMS medium containing 2.0 uM NAA , without charcoal.Complete plantlets. fNere successfully transplantedto the qlasshouse and qrown in hydroponic culture. Theplants flowered and produced normal fruit." @default.
- W44591738 created "2016-06-24" @default.
- W44591738 creator A5031247796 @default.
- W44591738 date "1989-05-01" @default.
- W44591738 modified "2023-09-26" @default.
- W44591738 title "In Vitro Culture of Muskmelon - Cucumis Melo Var. Birdie" @default.
- W44591738 hasPublicationYear "1989" @default.
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