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• W44902525 abstract "Research Article1 March 1986free access Subcellular localisation of the middle and large T-antigens of polyoma virus. S.M. Dilworth S.M. Dilworth Search for more papers by this author H.A. Hansson H.A. Hansson Search for more papers by this author C. Darnfors C. Darnfors Search for more papers by this author G. Bjursell G. Bjursell Search for more papers by this author C.H. Streuli C.H. Streuli Search for more papers by this author B.E. Griffin B.E. Griffin Search for more papers by this author S.M. Dilworth S.M. Dilworth Search for more papers by this author H.A. Hansson H.A. Hansson Search for more papers by this author C. Darnfors C. Darnfors Search for more papers by this author G. Bjursell G. Bjursell Search for more papers by this author C.H. Streuli C.H. Streuli Search for more papers by this author B.E. Griffin B.E. Griffin Search for more papers by this author Author Information S.M. Dilworth, H.A. Hansson, C. Darnfors, G. Bjursell, C.H. Streuli and B.E. Griffin The EMBO Journal (1986)5:491-499https://doi.org/10.1002/j.1460-2075.1986.tb04238.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The distribution of two of the polyoma virus early proteins (the large and middle T-antigens) in lytically infected mouse cells and transformed rat cells has been investigated by indirect immunofluorescence and immuno-electron microscopy using well-characterised monoclonal antibodies. By these techniques, the viral large T-antigen was found almost exclusively in the nucleus, sometimes in association with nuclear pores, but never in the nucleolus. In lytically infected, but not transformed cells, fluorescence was detected in discrete areas (‘hot spots’) within the nucleus and, in a minor population of lytically infected cells, cytoplasmic immunoreactive material was observed. The viral middle T-antigen was found in association with most cytoplasmic membranes and in the majority of cells mainly in the endoplasmic reticulum. Only a fraction of the staining was observed in the plasma membrane and no staining in the nucleoplasm was observed. The data suggest that the site of action of the major transforming activity of polyoma virus need not be at the plasma membrane. Functions associated with the viral antigens are discussed in terms of their subcellular distributions within cells. Previous ArticleNext Article Volume 5Issue 31 March 1986In this issue RelatedDetailsLoading ..." @default.
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• W44902525 title "Subcellular localisation of the middle and large T-antigens of polyoma virus." @default.
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• W44902525 doi "https://doi.org/10.1002/j.1460-2075.1986.tb04238.x" @default.
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