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• W44996576 abstract "This chapter discusses the use of cloned genomic DNA fragments as probes for the detection and identification of mollicutes. The probes are prepared by shotgun cloning of genomic DNA segments and selection of those segments that recognize relatively stable chromosomal regions discriminatory for the specific mollicute species. For fast and highly efficient construction of a genomic library to be used for the selection of DNA probes, the bacteriophage λGEM-12 with Xho Ihalf-site arms is used. The purified phage DNA are used directly as a DNA probe or restriction segments of the phage DNA insert are subcloned into a plasmid vector. Plasmid vectors have been routinely employed in the selection of DNA probes. The availability of genetically engineered commercial bacteriophage vectors provides an alternative way for selecting DNA probes. The procedure is very rapid, extremely efficient, and requires only small amounts of genomic DNA. The main advantage of this cloning strategy is that it makes genomic DNA fractionation and phosphorylation unnecessary. Within two rounds of plaque purification, a phage that does not contain highly conserved genes or other common sequences of a related mollicute species can be easily obtained." @default.
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• W44996576 date "1996-01-01" @default.
• W44996576 modified "2023-09-26" @default.
• W44996576 title "Cloned genomic DNA fragments as probes" @default.
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• W44996576 doi "https://doi.org/10.1016/b978-012583806-1/50115-2" @default.
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