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• W45102178 abstract "Research Article1 November 1986free access Sequences capable of restoring poly(A) site function define two distinct downstream elements. M.A. McDevitt M.A. McDevitt Search for more papers by this author R.P. Hart R.P. Hart Search for more papers by this author W.W. Wong W.W. Wong Search for more papers by this author J.R. Nevins J.R. Nevins Search for more papers by this author M.A. McDevitt M.A. McDevitt Search for more papers by this author R.P. Hart R.P. Hart Search for more papers by this author W.W. Wong W.W. Wong Search for more papers by this author J.R. Nevins J.R. Nevins Search for more papers by this author Author Information M.A. McDevitt, R.P. Hart, W.W. Wong and J.R. Nevins The EMBO Journal (1986)5:2907-2913https://doi.org/10.1002/j.1460-2075.1986.tb04586.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Several recent studies have shown that a functional poly(A) site consists of both an AAUAAA element as well as sequences downstream of the cleavage site. Two downstream regions were analyzed in an attempt to accurately locate and define the critical sequences. Chemically synthesized oligonucleotides of sequence from the early SV40 and the adenovirus E2A poly(A) sites were able to restore efficient cleavage to a deleted SV40 poly(A) site. Inversion of the sequence completely abolished poly(A) site function. A series of base substitution mutants were generated in each downstream sequence. Certain single base changes drastically altered poly(A) site function. Thus, it is concluded that a defined downstream sequence of limited complexity is important for efficient processing of the primary transcript at the poly(A) site. The position of the downstream elements relative to the AAUAAA and cleavage site was found to be critical since moving either the E2 element or the SV40 element an additional 40 nucleotides downstream abolished function. There were differences, however, in the effect of spacing on the function of the two elements. This observation, along with the fact that the two sequences are clearly different, indicates that there are at least two distinct genetic elements that direct efficient cleavage at the poly(A) site. Previous ArticleNext Article Volume 5Issue 111 November 1986In this issue RelatedDetailsLoading ..." @default.
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• W45102178 title "Sequences capable of restoring poly(A) site function define two distinct downstream elements." @default.
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