FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W45134365> ?p ?o ?g. }

• W45134365 abstract "Human cytomegalovirus (HCMV) is a ubiquitous virus of the herpes type that infects a high percentage of some populations. One of the most researched genes expressed by HCMV with close homology to human chemokine receptors is the US28 G protein-coupled receptor. Study design: This study was initiated to elucidate the intracellular signalling pathways of an inflammatory factor (IL-6) and an angiogenic factor (STAT3) triggered by the viral US28 oncogene and the presence of US28 in the HCMV viral particle. These pathways were observed by introducing the US28 gene into two human cell lines by infection with a HCMV strain that expresses the US28 gene (wild type), and two HCMV strains where the US28 gene was deleted (ΔUS28 and ΔUS28/UL33). Special attention was directed at the expression of IL-6 after promotion of the US28 gene and subsequent phosphorolation of STAT3. A new US28 antibody was validated and a method developed in an attempt to determine US28 on the viral particle. The following techniques were applied: Cell culture work, two mammalian cell lines were used, HFF’s and U373 MG. Virus stock titre determination to determine the multiplicity of infection. Protein quantitation to determine very small quantities of protein for Western blot analysis. ELISA for the quantitative determination of IL-6. Western blotting for phosphoSTAT3 determination and validation of the US28 antibody. Immunocytochemistry was used for back titrations of virally infected cells. Immunofluorescence assay and use of confocal microscopic techniques was used for the location of the US28 gene in the virion and for tSTAT3 translocation to the nucleus. Conclusion: A clear increase in IL-6 secretion (495% ± 1%) was seen, and this was after only an hour in HCMV WT infected cells. From the increase in IL-6 secretion a subsequent increase in STAT3 phosphorylation was detected in the same samples. A clear link has been established between IL-6 and STAT3. A method to determine whether US28 was present in the HCMV viral particle was designed and preliminary results obtained. The results were inclusive." @default.
• W45134365 created "2016-06-24" @default.
• W45134365 creator A5016546324 @default.
• W45134365 date "2011-06-23" @default.
• W45134365 modified "2023-09-27" @default.
• W45134365 title "The role of human cytomegalovirus encoded viral G protein-coupled receptors in onco-modulatory signalling" @default.
• W45134365 cites W122291100 @default.
• W45134365 cites W1445935732 @default.
• W45134365 cites W1528717967 @default.
• W45134365 cites W1531969743 @default.
• W45134365 cites W1554666404 @default.
• W45134365 cites W1601621203 @default.
• W45134365 cites W1638089104 @default.
• W45134365 cites W1763096406 @default.
• W45134365 cites W1780460389 @default.
• W45134365 cites W1788794972 @default.
• W45134365 cites W1836679973 @default.
• W45134365 cites W1858198414 @default.
• W45134365 cites W1942750313 @default.
• W45134365 cites W1955720256 @default.
• W45134365 cites W1955814101 @default.
• W45134365 cites W1963962908 @default.
• W45134365 cites W1968643760 @default.
• W45134365 cites W1971742580 @default.
• W45134365 cites W1972719386 @default.
• W45134365 cites W1972755060 @default.
• W45134365 cites W1975180353 @default.
• W45134365 cites W1976489150 @default.
• W45134365 cites W1977370355 @default.
• W45134365 cites W1978310995 @default.
• W45134365 cites W1981467511 @default.
• W45134365 cites W1982715030 @default.
• W45134365 cites W1983166456 @default.
• W45134365 cites W1985198089 @default.
• W45134365 cites W1991154912 @default.
• W45134365 cites W1994722134 @default.
• W45134365 cites W1994816216 @default.
• W45134365 cites W1995997868 @default.
• W45134365 cites W1998008325 @default.
• W45134365 cites W1999591184 @default.
• W45134365 cites W2000932088 @default.
• W45134365 cites W2006973742 @default.
• W45134365 cites W2007123668 @default.
• W45134365 cites W2008116181 @default.
• W45134365 cites W2008808301 @default.
• W45134365 cites W2012689489 @default.
• W45134365 cites W2013130521 @default.
• W45134365 cites W2016074757 @default.
• W45134365 cites W2016497689 @default.
• W45134365 cites W2017500382 @default.
• W45134365 cites W2026029378 @default.
• W45134365 cites W2026800814 @default.
• W45134365 cites W2030960461 @default.
• W45134365 cites W2031005807 @default.
• W45134365 cites W2031208709 @default.
• W45134365 cites W2033388874 @default.
• W45134365 cites W2037933291 @default.
• W45134365 cites W2037956917 @default.
• W45134365 cites W2039229115 @default.
• W45134365 cites W2041811554 @default.
• W45134365 cites W2042166361 @default.
• W45134365 cites W2045960597 @default.
• W45134365 cites W2052288973 @default.
• W45134365 cites W2053962612 @default.
• W45134365 cites W2056931664 @default.
• W45134365 cites W2057385062 @default.
• W45134365 cites W2057431892 @default.
• W45134365 cites W2058347762 @default.
• W45134365 cites W2062129127 @default.
• W45134365 cites W2062879283 @default.
• W45134365 cites W2067937110 @default.
• W45134365 cites W2073851843 @default.
• W45134365 cites W2074108669 @default.
• W45134365 cites W2079715612 @default.
• W45134365 cites W2082626794 @default.
• W45134365 cites W2086136868 @default.
• W45134365 cites W2087298678 @default.
• W45134365 cites W2090702421 @default.
• W45134365 cites W2091038079 @default.
• W45134365 cites W2098551575 @default.
• W45134365 cites W2098816998 @default.
• W45134365 cites W2100299146 @default.
• W45134365 cites W2101196984 @default.
• W45134365 cites W2103212167 @default.
• W45134365 cites W2103794276 @default.
• W45134365 cites W2108736984 @default.
• W45134365 cites W2115007851 @default.
• W45134365 cites W2118778038 @default.
• W45134365 cites W2125468282 @default.
• W45134365 cites W2128159409 @default.
• W45134365 cites W2130272830 @default.
• W45134365 cites W2131078803 @default.
• W45134365 cites W2134872350 @default.
• W45134365 cites W2139629103 @default.
• W45134365 cites W2140611891 @default.
• W45134365 cites W2141459099 @default.
• W45134365 cites W2149298399 @default.
• W45134365 cites W2150617763 @default.
• W45134365 cites W2151541220 @default.
• W45134365 cites W2153620197 @default.

• next